Widespread immunohistochemical staining of GPC3 in five different DN.

<p>(A ×400; B ×400; C ×400; D ×400; E ×400; F ×400).</p

Clinical information of the 20 female and 9 male patients enrolled in this study and the pathological features of their liver lesions.

<p><b>Note:</b>case 1–20, female patients; case 21–29, male patients.</p

Weak and focal immunohistochemical staining of GPC3 in several different DN.

<p>(A ×400; B ×400; C ×400; D ×400).</p

The LOH frequency of GPC3-positive and GPC3-negative DN at three loci.

<p>Fisher’s exact test demonstrated that the frequency of LOH was differentially higher in GPC3-positive DN compared to GPC3-negative DN at the above three loci.</p

GPC3 immunohistochemical staining in HCC.

<p>(Figure A ×200, B ×200, Figure C ×200, D ×400). A) weakly positive; B, C, and D) strongly positive.</p

The result of LOH analysis at D8S262 (A), D11S1301 (B) and D6S1008 (C):

<p>A) one or two bands from monoclonal GPC3-positive DN disappeared. These bands are present in normal liver tissues. N1-N8, normal liver tissue; N01-N08, 8 different monoclonal GPC3-positive DN.</p