Comparison of enteropathogen detection on 129 stool and swab sample pairs collected on the same day.

<p>Sensitivity and specificity of detection on swabs was calculated using the results from the corresponding stool as the reference.</p

Validation of qPCR assays on clinical samples, using confirmatory PCR assays and amplicon sequencing.

<p>The assays are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158199#pone.0158199.s001" target="_blank">S1 Table</a>.</p

Comparison of total nucleic acid extract versus 1:1 mixed DNA and RNA extracts.

<p>For the RNA derived targets below, we compared Cqs between nucleic acid extracted with QIAamp Stool DNA mini kit versus a 1:1 mixture of QIAamp stool DNA mini kit extract and FUJIfilm QuickGene RNA tissue kit or QIAamp viral RNA mini kit extract.</p

Demonstration of quantification on spiked analytical samples.

<p>Five lots of stool with different amount of inhibitors were prepared, then the mixture of target nucleic acid was spiked. Extraction and testing with TAC were performed, target copy numbers were calculated based on standard curves and normalized to extrinsic controls. Target copy numbers (circles) were log2 transformed in order to be on the same scale as Cq.</p