Additional file 2: Figure S1. of The effect of adipose tissue-derived stem cells in a middle cerebral artery occlusion stroke model depends on their engraftment rate

Influence of VSOP labelling on cell vitality and growth behaviour. LDH release did not differ for unlabelled (negative control) and VSOP-labelled ASCs. Both differed significantly from positive control. *p < 0.05 for negative control and VSOP-labelled cells vs. positive control (A). Growth curves and fraction of dead cells did not differ between labelled and unlabelled cells at any time (diagram). Growth curves of vital cells increased significantly during the observation period. VSOP density in cytoplasm decreased within 9 days (images). *p < 0.05 for increased cell growth up to 14 days for VSOP-labelled and unlabelled ASCs (B). ASC adipose tissue-derived stem cell, LDH lactate dehydrogenase, VSOP very small superparamagnetic iron oxide particles. (TIF 6166 kb

Additional file 4: Figure S3. of The effect of adipose tissue-derived stem cells in a middle cerebral artery occlusion stroke model depends on their engraftment rate

Identification and viability testing of ASCs via staining of human mitochondria and Ki67. Representative images of PB-positive areas (left images) co-localized with locations positive for human mitochondria (B) and Ki67 (C) (DAB-positive signal, middle images) in neighbour slices whereas negative controls showed no DAB signal (right images). ASC adipose tissue-derived stem cell, DAB 3,3'-diaminobenzidine, PB Prussian blue. (TIF 7971 kb