Expansion of epicyclic gear dynamic analysis program

The multiple mesh/single stage dynamics program is a gear tooth analysis program which determines detailed geometry, dynamic loads, stresses, and surface damage factors. The program can analyze a variety of both epicyclic and single mesh systems with spur or helical gear teeth including internal, external, and buttress tooth forms. The modifications refine the options for the flexible carrier and flexible ring gear rim and adds three options: a floating Sun gear option; a natural frequency option; and a finite element compliance formulation for helical gear teeth. The option for a floating Sun incorporates two additional degrees of freedom at the Sun center. The natural frequency option evaluates the frequencies of planetary, star, or differential systems as well as the effect of additional springs at the Sun center and those due to a flexible carrier and/or ring gear rim. The helical tooth pair finite element calculated compliance is obtained from an automated element breakup of the helical teeth and then is used with the basic gear dynamic solution and stress postprocessing routines. The flexible carrier or ring gear rim option for planetary and star spur gear systems allows the output torque per carrier and ring gear rim segment to vary based on the dynamic response of the entire system, while the total output torque remains constant

Conformational Change in the Chromatin Remodelling Protein MENT

Chromatin condensation to heterochromatin is a mechanism essential for widespread suppression of gene transcription, and the means by which a chromatin-associated protein, MENT, induces a terminally differentiated state in cells. MENT, a protease inhibitor of the serpin superfamily, is able to undergo conformational change in order to effect enzyme inhibition. Here, we sought to investigate whether conformational change in MENT is ‘fine-tuned’ in the presence of a bound ligand in an analogous manner to other serpins, such as antithrombin where such movements are reflected by a change in intrinsic tryptophan fluorescence. Using this technique, MENT was found to undergo structural shifts in the presence of DNA packaged into nucleosomes, but not naked DNA. The contribution of the four Trp residues of MENT to the fluorescence change was mapped using deconvolution analysis of variants containing single Trp to Phe mutations. The analysis indicated that the overall emission spectra is dominated by a helix-H tryptophan, but this residue did not dominate the conformational change in the presence of chromatin, suggesting that other Trp residues contained in the A-sheet and RCL regions contribute to the conformational change. Mutagenesis revealed that the conformational change requires the presence of the DNA-binding ‘M-loop’ and D-helix of MENT, but is independent of the protease specificity determining ‘reactive centre loop’. The D-helix mutant of MENT, which is unable to condense chromatin, does not undergo a conformational change, despite being able to bind chromatin, indicating that the conformational change may contribute to chromatin condensation by the serpin

Seismic Coupling of Short-Period Wind Noise Through Mars’ Regolith for NASA’s InSight Lander

NASA’s InSight lander will deploy a tripod-mounted seismometer package onto the surface of Mars in late 2018. Mars is expected to have lower seismic activity than the Earth, so minimisation of environmental seismic noise will be critical for maximising observations of seismicity and scientific return from the mission. Therefore, the seismometers will be protected by a Wind and Thermal Shield (WTS), also mounted on a tripod. Nevertheless, wind impinging on the WTS will cause vibration noise, which will be transmitted to the seismometers through the regolith (soil). Here we use a 1:1-scale model of the seismometer and WTS, combined with field testing at two analogue sites in Iceland, to determine the transfer coefficient between the two tripods and quantify the proportion of WTS vibration noise transmitted through the regolith to the seismometers. The analogue sites had median grain sizes in the range 0.3–1.0 mm, surface densities of 1.3-1.8 gcm−3, and an effective regolith Young’s modulus of 2.5+1.9−1.4 MPa. At a seismic frequency of 5 Hz the measured transfer coefficients had values of 0.02–0.04 for the vertical component and 0.01–0.02 for the horizontal component. These values are 3–6 times lower than predicted by elastic theory and imply that at short periods the regolith displays significant anelastic behaviour. This will result in reduced short-period wind noise and increased signal-to-noise. We predict the noise induced by turbulent aerodynamic lift on the WTS at 5 Hz to be ∼2×10−10 ms−2 Hz−1/2 with a factor of 10 uncertainty. This is at least an order of magnitude lower than the InSight short-period seismometer noise floor of 10−8 ms−2 Hz−1/2