Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing

Preservation of cancer biopsies by FFPE introduces DNA fragmentation, hindering analysis of rearrangements. Here the authors introduce FFPE Targeted Locus Capture for identification of translocations in preserved samples.In routine diagnostic pathology, cancer biopsies are preserved by formalin-fixed, paraffin-embedding (FFPE) procedures for examination of (intra-) cellular morphology. Such procedures inadvertently induce DNA fragmentation, which compromises sequencing-based analyses of chromosomal rearrangements. Yet, rearrangements drive many types of hematolymphoid malignancies and solid tumors, and their manifestation is instructive for diagnosis, prognosis, and treatment. Here, we present FFPE-targeted locus capture (FFPE-TLC) for targeted sequencing of proximity-ligation products formed in FFPE tissue blocks, and PLIER, a computational framework that allows automated identification and characterization of rearrangements involving selected, clinically relevant, loci. FFPE-TLC, blindly applied to 149 lymphoma and control FFPE samples, identifies the known and previously uncharacterized rearrangement partners. It outperforms fluorescence in situ hybridization (FISH) in sensitivity and specificity, and shows clear advantages over standard capture-NGS methods, finding rearrangements involving repetitive sequences which they typically miss. FFPE-TLC is therefore a powerful clinical diagnostics tool for accurate targeted rearrangement detection in FFPE specimens.Immunobiology of allogeneic stem cell transplantation and immunotherapy of hematological disease

Oncologist, patient, and companion questions during pretreatment consultations about adjuvant cancer treatment: a shared decision-making perspective

Experimentele farmacotherapi

Ethnicity or tribalism? : The discursive construction of Zimbabwean national identity

In Zimbabwe any attempt to discuss ethnicity risks being labelled as ‘tribalism’ and, therefore, divisive to a supposedly ‘united nation.’ But what is ethnicity? This paper will attempt to discuss this issue, with particular focus on its intersection with nationalism and the construction of national identity. It will illuminate the liminal process of the ‘criminalisation of ethnicity’ through some moves aimed at blocking open discourse on ethnicity as a form of identity. Furthermore, the paper illustrates how Zimbabwe’s Shona-dominated nationalist discourses tend to follow the social constructivist path, which publicly dismisses the existence of ethnicity while clandestinely embracing it for dubious political purposes. In the process, the paper will also challenge the Eurocentric-theoretical perspectives underlying the normative engagement of ethnicity within the political and culturalist perspectives in Africa for presenting ethnicity as retrogressive and divisive. It is further argued that the scarcity of indigenous theoretical lenses of understanding ethnicity, exacerbated by grotesque forms of nationalism, as seen in Zimbabwe and elsewhere in Africa sustains the suppression of ethnic minority voices and the shrift dismissal of their issues as peripheral or regional. This lack of proper vent has thus led to the continued resurgence of violent ethnic upheavals across the African continent.Peer reviewe