Evolution of cell density and identification of growth stages before DNA staining.

<p>Five growth stages were defined according to log OD of the cell culture: 1: lag phase (black), 2: start of growth (cyan), 3: exponential stage (green), 4: slowdown phase (orange), 5: stationary phase (red).</p

Evolution of the cytometric signal during cell culture (time in min) with A: cell size (Forward SCatter), B: SYTOX® Green, C: propidium iodide (PI), D: TO-PRO-3, E: 7-aminoactinomycin D (7-AAD) and F: SYBR® Green I.

<p>The x axes are in arbitrary units. The y axes correspond to relative frequencies adjusted for each signal. Each histogram represents about 20000 <i>cells</i> Colors correspond to the different growth stages as defined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084645#pone-0084645-g002" target="_blank">Figure 2</a>. For B to F, the vertical lines indicates the median signal (coefficient of variation) corresponding strictly to yeast cells at G1 stage during the exponential phase.</p

One dimensional representation of the apparent cell size (A, B), and DNA content as revealed by SYTOX Green (C, D) and SYBR Green I (E, F) labeling.

<p>Arrows underline the main evolutions of the DNA content from the lag phase to exponential growth (A, C, E) and from exponential growth to the stationary phase (B, D, F). Colors correspond to the different stages as defined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084645#pone-0084645-g002" target="_blank">Figure 2</a>.</p

Signal intensity with different fluorochromes during the exponential cell growth phase (G1 and G2/M) or stationary phase (G0).

<p>Global signal of low quality and upper G0 fluorescence.</p><p>^y presence of a sub-G1 peak out of the exponential growth.</p><p>ⁿ absence of a sub-G1 peak out of the exponential growth.</p

Time courses of the relative proportions of the cell populations during the various cell cycle stages, using SYTOX® Green fluorochrome (G0: black; G1: purple; S: green and G2/M: magenta).

<p>Colored scale indicates the stages as defined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084645#pone-0084645-g002" target="_blank">Figure 2</a>.</p

Budding yeast cell progression through the cell cycle.

<p>Budding yeast cell progression through the cell cycle.</p

GO term annotations for genes significantly down-regulated in the 59A sch9-deleted mutant.

<p>k: number of genes in the family identified as affected in the experiment; f: total number of genes in the family.</p

Effect of nitrogen source on the viability of <i>S. cerevisiae</i> EC1118 cells during alcoholic fermentation.

<p>The synthetic medium contained 71 mg/L assimilable nitrogen (SM71) and 5% lipid factors (LF5%), with or without additional arginine, glutamine, glutamate, histidine or proline (142 mg/L assimilable nitrogen final content as in SM142). Viability was measured by flow cytometry after propidium iodide staining. The graphs are the result of smoothing of measurements series (at least 3 repetitions) using the software R. Smoothing obtained is framed by a confidence interval calculated at 95%.</p

Effect of Sch9 deletion on the cell viability of haploid <i>S. cerevisiae</i> 59A during alcoholic fermentation.

<p>The synthetic medium contains 71 mg/L (SM71) or 142 mg/L (SM142) of assimilable nitrogen, and 5% lipid factor (LF5%). Viability was measured by flow cytometry after propidium iodide staining. The graphs are the result of smoothing of measurements series (at least 3 repetitions) using the software R. Smoothing obtained is framed by a confidence interval calculated at 95%.</p

Trehalose and glycogen contents at stationary phase of <i>S. cerevisiae</i> EC1118.

<p>Cells were fermented in synthetic medium containing 71 mg/L (SM71) or 142 mg/L (SM142) assimilable nitrogen, and 5% or 100% lipid factors (LF5% or LF100%).</p