Endothelial Dysfunction Criteria in Critically Ill Children: The PODIUM Consensus Conference

OBJECTIVES To review, analyze, and synthesize the literature on endothelial dysfunction in critically ill children with multiple organ dysfunction syndrome and to develop a consensus biomarker-based definition and diagnostic criteria. DATA SOURCES Electronic searches of PubMed and Embase were conducted from January 1992 to January 2020, using a combination of medical subject heading terms and key words to define concepts of endothelial dysfunction, pediatric critical illness, and outcomes. STUDY SELECTION Studies were included if they evaluated critically ill children with endothelial dysfunction, evaluated performance characteristics of assessment/scoring tools to screen for endothelial dysfunction, and assessed outcomes related to mortality, functional status, organ-specific outcomes, or other patient-centered outcomes. Studies of adults or premature infants (≤36 weeks gestational age), animal studies, reviews or commentaries, case series with sample size ≤10, and non-English language studies with the inability to determine eligibility criteria were excluded. DATA EXTRACTION Data were abstracted from each eligible study into a standard data extraction form along with risk of bias assessment. DATA SYNTHESIS We identified 62 studies involving 84 assessments of endothelial derived biomarkers indirectly linked to endothelial functions including leukocyte recruitment, inflammation, coagulation, and permeability. Nearly all biomarkers studied lacked specificity for vascular segment and organ systems. Quality assessment scores for the collected literature were low. CONCLUSIONS The Endothelial Subgroup concludes that there exists no single or combination of biomarkers to diagnose endothelial dysfunction in pediatric multiple organ dysfunction syndrome. Future research should focus on biomarkers more directly linked to endothelial functions and with specificity for vascular segment and organ systems

A Curriculum for a Master of Science in Information Quality

The first Master of Science in Information Quality (IQ) degree is designed and being offered to prepare students for careers in industry and government as well as advanced graduate studies. The curriculum is guided by the Model Curriculum and Guidelines for Graduate Degree Programs in Information Systems, which are endorsed by the Association for Computing Machinery and the Association for Information Systems. The curriculum integrates two key educational innovations: (1) an interdisciplinary approach to curriculum design, and (2) a balance between theoretical rigor and practical relevance. In response to the demand from industry, the curriculum aims to educate students who can lead the effort to solve current and future information quality problems. As such, problem-based learning is balanced with foundation-building learning to effectively deliver the intellectual contents of the curriculum. Much of the individual course content is based on cumulated research results and practices developed over the last two decades. The curriculum is designed to balance information quality theory with industry best practices using modern tools and technology. It includes the skill sets that are critical to succeed as IQ professionals. Since IQ is an inter-disciplinary field, the curriculum draws upon total quality management, database, core knowledge of IQ, change management, project management, and IQ policy and strategy. The courses are delivered using case studies, hands-on laboratories, theory building, and team projects to enhance the student\u27s learning experience. Upon completing the program, students will be equipped with sufficient breadth and depth in the IQ field to solve real world problems and pursue further studies

Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat)

Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The objective of this research was to investigate potential immunotoxic effects of brevetoxins using a leukemic T cell line (Jurkat) as an in vitro model system. Viability, cell proliferation, and apoptosis assays were conducted using brevetoxin congeners PbTx-2, PbTx-3, and PbTx-6. The effects of in vitro brevetoxin exposure on cell viability and cellular metabolism or proliferation were determined using trypan blue and MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan), respectively. Using MTT, cellular metabolic activity was decreased in Jurkat cells exposed to 5 – 10 μg/ml PbTx-2 or PbTx-6. After 3 h, no significant effects on cell viability were observed with any toxin congener in concentrations up to 10 μg/ml. Viability decreased dramatically after 24 h in cells treated with PbTx-2 or -6. Apoptosis, as measured by caspase-3 activity, was significantly increased in cells exposed to PbTx-2 or PbTx-6. In summary, brevetoxin congeners varied in effects on Jurkat cells, with PbTx-2 and PbTx-6 eliciting greater cellular effects compared to PbTx-3

The Ursa Major Cluster of Galaxies. I. Cluster Definition and Photometric Data

The Ursa Major Cluster has received remarkably little attention, although it is as near as the Virgo Cluster and contains a comparable number of HI-rich galaxies. In this paper, criteria for group membership are discussed and data are presented for 79 galaxies identified with the group. Of these, all 79 have been imaged at B,R,I bands with CCDs, 70 have been imaged at K' with a HgCdTe array detector, and 70 have been detected in the HI 21cm line. A complete sample of 62 galaxies brighter than M(B)=-16.5 is identified. Images and gradients in surface brightness and color are presented at a common linear scale. As has been seen previously, the galaxies with the reddest global colors are reddest at the centers and get bluer at large radii. However, curiously, among the galaxies with the bluest global colors there are systems with very blue cores that get redder at large radii.Comment: A LATEX file without figures. The postscript version (7.1Mb in gzipped format) including all the tables, figures and scanned versions of the plates can be retrieved as preprint no.208 from http://www.astro.rug.nl:80/~secr/ Accepted for publication in The Astronomical Journa

Space Station Engineering Design Issues

Space Station Freedom topics addressed include: general design issues; issues related to utilization and operations; issues related to systems requirements and design; and management issues relevant to design

Sequence features and chromatin structure around the genomic regions bound by 119 human transcription factors

Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the dominant technique for mapping transcription factor (TF) binding regions genome-wide. We performed an integrative analysis centered around 457 ChIP-seq data sets on 119 human TFs generated by the ENCODE Consortium. We identified highly enriched sequence motifs in most data sets, revealing new motifs and validating known ones. The motif sites (TF binding sites) are highly conserved evolutionarily and show distinct footprints upon DNase I digestion. We frequently detected secondary motifs in addition to the canonical motifs of the TFs, indicating tethered binding and cobinding between multiple TFs. We observed significant position and orientation preferences between many cobinding TFs. Genes specifically expressed in a cell line are often associated with a greater occurrence of nearby TF binding in that cell line. We observed cell-line-specific secondary motifs that mediate the binding of the histone deacetylase HDAC2 and the enhancer-binding protein EP300. TF binding sites are located in GC-rich, nucleosome-depleted, and DNase I sensitive regions, flanked by well-positioned nucleosomes, and many of these features show cell type specificity. The GC-richness may be beneficial for regulating TF binding because, when unoccupied by a TF, these regions are occupied by nucleosomes in vivo. We present the results of our analysis in a TF-centric web repository Factorbook (http://factorbook.org) and will continually update this repository as more ENCODE data are generated