Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

International audienceCurrently there is considerable interest in creating ordered arrays of adhesive protein islands in a sea of passivated surface for cell biological studies. In the past years, it has become increasingly clear that living cells respond, not only to the biochemical nature of the molecules presented to them but also to the way these molecules are presented. Creating protein micro-patterns is therefore now standard in many biology laboratories; nano-patterns are also more accessible. However, in the context of cell-cell interactions, there is a need to pattern not only proteins but also lipid bilayers. Such dual proteo-lipidic patterning has so far not been easily accessible. We offer a facile technique to create protein nano-dots supported on glass and propose a method to backfill the inter-dot space with a supported lipid bilayer (SLB). From photo-bleaching of tracer fluorescent lipids included in the SLB, we demonstrate that the bilayer exhibits considerable in-plane fluidity. Functionalizing the protein dots with fluorescent groups allows us to image them and to show that they are ordered in a regular hexagonal lattice. The typical dot size is about 800 nm and the spacing demonstrated here is 2 microns. These substrates are expected to serve as useful platforms for cell adhesion, migration and mechano-sensing studies

Nanometric Protein-Patch Arrays on Glass and Polydimethylsiloxane for Cell Adhesion Studies

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Nano-clustering of ligands on synthetic APCs influences T-cell membrane and actin organization

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Review of broadband metamaterial absorbers: from principles, design strategies, and tunable properties to functional applications

Metamaterial absorbers have been widely studied and continuously concerned owing to their excellent resonance features of ultra-thin thickness, light-weight, and high absorbance. Their applications, however, are typically restricted by the intrinsic dispersion of materials and strong resonant features of patterned arrays (mainly referring to narrow absorption bandwidth). It is, therefore essential to reassert the principles of building broadband metamaterial absorbers (BMAs). Herein, the research progress of BMAs from principles, design strategies, tunable properties to functional applications are comprehensively and deeply summarized. Physical principles behind broadband absorption are briefly discussed, typical design strategies in realizing broadband absorption are further emphasized, such as top-down lithography, bottom-up self-assembly, and emerging 3D printing technology. Diversified active components choices, including optical response, temperature response, electrical response, magnetic response, mechanical response, and multi-parameter responses, are reviewed in achieving dynamically tuned broadband absorption. Following this, the achievements of various interdisciplinary applications for BMAs in energy-harvesting, photodetectors, radar-IR dual stealth, bolometers, noise absorbing, imaging, and fabric wearable are summarized. Finally, the challenges and perspectives for future development of BMAs are discussed.This research was funded by the National Natural Science Foundation of China (62105128), the China Scholarship Council (202106795002), and the Key Lab of Modern Optical Technologies of Education Ministry of China, Soochow University

Extraction, Purification, Structural Characteristics, Biological Activities and Pharmacological Applications of Acemannan, a Polysaccharide from <i>Aloe vera</i>: A Review

Aloe vera is a medicinal plant species of the genus Aloe with a long history of usage around the world. Acemannan, considered one of the main bioactive polysaccharides of Aloe vera, possesses immunoregulation, anti-cancer, anti-oxidation, wound healing and bone proliferation promotion, neuroprotection, and intestinal health promotion activities, among others. In this review, recent advancements in the extraction, purification, structural characteristics and biological activities of acemannan from Aloe vera were summarized. Among these advancements, the structural characteristics of purified polysaccharides were reviewed in detail. Meanwhile, the biological activities of acemannan from Aloe vera determined by in vivo, in vitro and clinical experiments are summarized, and possible mechanisms of these bioactivities were discussed. Moreover, the latest research progress on the use of acemannan in dentistry and wound healing was also summarized in details. The structure-activity relationships of acemannan and its medical applications were discussed. Finally, new perspectives for future research work on acemannan were proposed. In conclusion, this review summarizes the extraction, purification, structural characteristics, biological activities and pharmacological applications of acemannan, and provides information for the industrial production and possible applications in dentistry and wound healing in the future

Nano-clustering of ligands on surrogate antigen presenting cells modulates T cell membrane adhesion and organization

International audienceWe investigate the adhesion and molecular organization of the plasma membrane of T lymphocytes interacting with a surrogate antigen presenting cell comprising glass supported ordered arrays of antibody (alpha-CD3) nano-dots dispersed in a non-adhesive matrix of polyethylene glycol (PEG). The local membrane adhesion and topography, as well as the distribution of the T cell receptors (TCRs) and the kinase ZAP-70, are influenced by dot-geometry, whereas the cell spreading area is determined by the overall average density of the ligands rather than specific characteristics of the dots. TCR clusters are recruited preferentially to the nano-dots and the TCR cluster size distribution has a weak dot-size dependence. On the patterns, the clusters are larger, more numerous, and more enriched in TCRs, as compared to the homogeneously distributed ligands at comparable concentrations. These observations support the idea that non-ligated TCRs residing in the non-adhered parts of the proximal membrane are able to diffuse and enrich the existing clusters at the ligand dots. However, long distance transport is impaired and cluster centralization in the form of a central supramolecular cluster (cSMAC) is not observed. Time-lapse imaging of early cell-surface contacts indicates that the ZAP-70 microclusters are directly recruited to the site of the antibody dots and this process is concomitant with membrane adhesion. These results together point to a complex interplay of adhesion, molecular organization and activation in response to spatially modulated stimulation

A Competitive “On-Off-Enhanced On” AIE Fluorescence Switch for Detecting Biothiols Based on Hg²⁺ Ions and Gold Nanoclusters

In this study, a novel “on-off-enhanced on” approach to highly sensitive rapid sensing of biothiols was developed, based on competitive modulation of gold nanoclusters (AuNCs) and Hg2+ ions. In our approach, the AuNCs were encapsulated into a zeolite imidazole framework (ZIF) for predesigned competitive aggregation-induced luminescence (AIE) emission. To readily operate this approach, the Hg2+ ions were selected as mediators to quench the fluorescence of AuNCs. Then, due to the stronger affinities between the interactions of Hg2+ ions with -SH groups in comparison to the AuNCs with -SH groups, the quenched probe of AuNCs@ZIF-8/Hg2+ displayed enhanced fluorescence after the Hg2+ ions were competitively interacted with -SH groups. Based on enhanced fluorescence, the probe for AuNCs@ZIF-8/Hg2+ had a sensitive and specific response to trace amounts of biothiols. The developed fluorescence strategy had limit of quantification (LOQ) values of 1.0 μM and 1.5 μM for Cys and GSH molecules in serum, respectively. This competitive AIE strategy provided a new direction for developing biological probes and a promising method for quantifying trace amounts of biothiols in serum. It could promote progress in disease diagnosis

Design of multiple-frequency-band terahertz metamaterial absorbers with adjustable absorption peaks using toothed resonator

Multiple-frequency-band metamaterial absorbers possess great application prospects, which are usually achieved by vertically stacking or coplanar arranging several sub-resonators. Obtaining more absorption peaks requires further sacrifice of the number of sub-resonators. More importantly, these two design methods are difficult to control or adjust the number of absorption peaks without changing the number of sub-resonators. Therefore, new scheme using simplified structure without increasing any design complexity to realize multiple-frequency-band absorption with adjustable resonance features is urgently needed. In this paper, a multiple-frequency-band terahertz metamaterial absorber using surface structure of toothed resonator is demonstrated, it has the ability to control (increase or decrease) the number of absorption peaks without increasing its design complexity, which is different from previous works that need to sacrifice the design complexity of metamaterials. Furthermore, the introduction of temperature-controlled vanadium dioxide into the surface structure of multiple-frequency-band absorber can dynamically tune its resonance performance. It is proved that when vanadium dioxide changes from metallic state to insulating state, its absorption peaks can be actively adjusted from dual- to triple-, quad- and even penta-frequency-band absorption. These efforts could provide meaningful guidance for the design of multiple-frequency-band metamaterial absorbers, and could have broad application prospects in terahertz technology-related areas

Investigation into Cellular Glycolysis for the Mechanism Study of Energy Metabolism Disorder Triggered by Lipopolysaccharide

Lipopolysaccharide (LPS) is the main virulence factor of Gram-negative bacteria, which can incite inflammation in tissues by inducing cells to secrete a variety of proinflammatory mediators, including cytokines, chemokines, interleukins, and prostaglandins. Herein, we chose LPS as an inducer to establish an inflammatory model of HeLa cells, and explored the effects of LPS on energy metabolism. We treated HeLa cells with different concentrations (0, 0.4, 1.0, 2.0, 4.0, and 6.0 &#956;g/mL) of LPS for 24 h, and explored its effects on intercellular adenosine triphosphate (ATP) levels, intercellular nitrous oxide (NO) content, mitochondrial functions, and enzyme activities related to energy metabolism. Furthermore, we used metabonomics to study the metabolites that participated in energy metabolism. We found a positive correlation between LPS concentrations and intracellular ATP levels. In addition, LPS increased intracellular NO production, altered mitochondrial functions, strengthened glycolytic enzyme activities, and changed metabolites related to energy metabolism. Hence, in this study, we showed that LPS can strengthen energy metabolism by enhancing glycolysis, which could be used as an early diagnostic biomarker or a novel therapeutic target for inflammation-associated cancers