CD27 and Mip-1β expression of CMV specific IFNγ producing CD4 T cell responses are influenced by HIV as well as tuberculosis co-infection in adults.

<p>Scatter-plots illustrating (A) the CD27 MFI fold-change to all CD4⁺ T cells and B) the proportion of cells producing Mip-1β of CMV specific IFNγ⁺-CD4⁺ T cell in patients under 10 or over 18 years old and according to HIV and TB infection status.</p

CD27 and Mip-1β expression of CMV specific IFNγ producing CD8 T cell responses are not significantly influenced by HIV as well as tuberculosis co-infection in adults.

<p>Scatter-plots illustrating (A) the CD27 MFI fold-change to all CD8⁺ T cells and B) the proportion of cells producing Mip-1β of CMV specific IFNγ⁺-CD8⁺ T cell in patients under 10 or over 18 years old and according to HIV and TB infection status.</p

Frequencies and absolute counts of IFNγ⁺-CMVpp65–specific CD4⁺ or CD8⁺ T cell in children below 10 years of age and adults.

<p>Scatter-plots depicting A) the frequencies or absolute counts of CMV specific IFNγ⁺-CD4⁺ T cells s and C) frequencies of CMV specific IFNγ⁺-CD8⁺ T cells in the two-age groups and according to HIV status.</p

Frequencies and absolute counts of IFNγ⁺-SEB–specific CD4⁺ or CD8⁺ T cell in children below 10 years of age and adults.

<p>Scatter-plots depicting A) the frequencies or B) absolute counts of SEB specific IFNγ⁺-CD4⁺ T cells s and C) frequencies of SEB specific IFNγ⁺-CD8⁺ T cells in the two-age groups and according to HIV status.</p

CD27 and Mip-1β expression of CMV specific IFNγ producing CD4 T cell responses are influenced by HIV as well as tuberculosis co-infection in adults.

<p>Scatter-plots illustrating (A) the CD27 MFI fold-change to all CD4⁺ T cells and B) the proportion of cells producing Mip-1β of CMV specific IFNγ⁺-CD4⁺ T cell in patients under 10 or over 18 years old and according to HIV and TB infection status.</p

Gating strategies and CMV responses evaluation.

<p>A) Morphological and CD marker expression gating strategy of CD4 and CD8 T cell. B) Representative CD4 and CD8 T cell IFNγ response from a significant responder. C) Gating strategy for the CD27 MFI Fold Change calculation of IFNγ producing cells compared to all CD4 or CD8 T cells. D) Gating strategy for the determination of IFNγ producing cells that were also positive for Mip1β production.</p