Additional file 4: of Absolute quantitation of microbiota abundance in environmental samples

Figure S1. Model of synthetic spike addition. Number of sequencing reads of synthetic spike per 1000 total reads (y axis) from 16 s rRNA, 18S rRNA and ITS following addition to soil of different levels of P, E and F synthetic spikes (x axis). The figure presents the full dataset from the averaged dataset shown on Fig. 3. (TXT 39 kb

Additional file 7: of Absolute quantitation of microbiota abundance in environmental samples

Description of samples submitted to EBI server. (XLSX 74 kb

Additional file 9: of Absolute quantitation of microbiota abundance in environmental samples

Modified Bash code including Unoise3 command. (XLSX 422 kb

Additional file 3: of Absolute quantitation of microbiota abundance in environmental samples

Data used for figures and tables preparation. (XLSX 54 kb

Additional file 8: of Absolute quantitation of microbiota abundance in environmental samples

Bash code for the sequencing analysis. (XLSX 63 kb

Additional file 1: of Absolute quantitation of microbiota abundance in environmental samples

Experiment preparation details: spike sequences, primers sequences, Rhizobium experiment spike output and spike concentration to numbers calculation. (XLSX 17 kb

Additional file 6: of Absolute quantitation of microbiota abundance in environmental samples

qPCR data. (TXT 12 kb

Additional file 2: of Absolute quantitation of microbiota abundance in environmental samples

Length of the sequences obtained for Bawburgh vs. Wytham soil comparison. (PDF 133 kb

AIB transport by Rlv3841 and AA031 (Δ<i>npr</i>) and AA031 complemented with the empty vector pRK415 (EV), NPr, expressed from the constitutive lac promoter in multi-copy (+NPr) or a non-phosphorylatable variant (+NPrH17A).

<p>Data are averages (± SEM) from ≥3 independent cultures and rates are nmol min⁻¹ mg protein⁻¹.</p

AIB membrane transport by Rlv3841, PtsP107 (ptsP::Tn5) and various phosphocarrier protein mutants.

<p>Data are averages (± SEM) from 6 independent cultures and rates are nmol min⁻¹mg protein⁻¹.</p