4,951 research outputs found
Arctic Plants, Ecosystems and Strategies
Reviews and develops a perspective of what is known about the structure, function, and adaptive strategy of arctic tundra ecosystems, with emphasis on plants, the primary biological producers of an ecosystem. The short-term change in plant arrays following disturbance of the natural assemblage, due to ecological succession, is poorly understood in the tundra. Distribution and migrations of tundra flora give insight into Pleistocene events and evolutionary strategies, one clue to which is frequently of polyploidy. Implicit in understanding tundra dynamics or vegetation associations is study of topographic microrelief, soils and thaw depths, as well as description of the flora. Progress is noted in knowledge of the structure and function of vegetation in arctic ecosystems: the morphological adaptation, carbohydrate cycle, chlorophyll content, physiologic processes in adaption to severe environments such as photosynthesis, respiration, light saturation, photoperiodic requirements and temperature tolerance
Laramide basin subsidence and fluvial architecture of the Fort Union and Wasatch Formations, Southern Green River Basin, Wyoming
Panic-prone state induced in rats with GABA dysfunction in the dorsomedial hypothalamus is mediated by NMDA receptors
Rats with chronic inhibition of GABA synthesis and consequently enhanced glutamatergic excitation in the dorsomedial hypothalamus (DMH) develop panic-like responses, defined as tachycardia, tachypnea, hypertension, and increased anxiety as measured by a social interaction (SI) test, after intravenous sodium lactate infusions, a phenomenon similar to patients with panic disorder. Therefore, the present studies tested the role of the postsynaptic NMDA and AMPA type glutamatergic receptors in the lactate-induced panic-like responses in these rats. Rats were fit with femoral arterial and venous catheters and Alzet pumps [filled with the GABA synthesis inhibitor L-allylglycine (L-AG; 3.5 nmol/0.5 microl per hour) or its inactive isomer D-AG] into the DMH. After 4-5 d of recovery only those rats with L-AG pumps exhibited panic-like responses to lactate infusions. Using double immunocytochemistry, we found that rats exhibiting panic-like responses (e.g., L-AG plus lactate) had increased c-Fos immunoreactivity in DMH neurons expressing the NMDA receptor 1 (NR1) subunit, but not those expressing the glutamate receptor 2 and 3 subunits of the AMPA receptors. To confirm this pharmacologically, we tested another group of rats implanted with l-AG pumps with intravenous lactate infusions preceded by injections of either NMDA [aminophosphonopentanoic acid (AP-5) or (+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d]cyclohepten-5,10-imine maleate (MK-801)] or non-NMDA [CNQX or 4-(8-methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodazepin-5-yl)-benzenamine dihydrochloride (GYKI52466)] antagonists into the DMH. Injections of NMDA, but not non-NMDA, antagonists into the DMH resulted in dose-dependent blockade of the tachycardia, tachypnea, hypertension, and SI responses after lactate infusions. These results suggest that NMDA, and not non-NMDA, type glutamate receptors regulate lactate-induced panic-like responses in rats with GABA dysfunction in the DMH
Inference of Microbial Recombination Rates from Metagenomic Data
Metagenomic sequencing projects from environments dominated by a small number of species produce genome-wide population samples. We present a two-site composite likelihood estimator of the scaled recombination rate, ρ = 2Nec, that operates on metagenomic assemblies in which each sequenced fragment derives from a different individual. This new estimator properly accounts for sequencing error, as quantified by per-base quality scores, and missing data, as inferred from the placement of reads in a metagenomic assembly. We apply our estimator to data from a sludge metagenome project to demonstrate how this method will elucidate the rates of exchange of genetic material in natural microbial populations. Surprisingly, for a fixed amount of sequencing, this estimator has lower variance than similar methods that operate on more traditional population genetic samples of comparable size. In addition, we can infer variation in recombination rate across the genome because metagenomic projects sample genetic diversity genome-wide, not just at particular loci. The method itself makes no assumption specific to microbial populations, opening the door for application to any mixed population sample where the number of individuals sampled is much greater than the number of fragments sequenced
Screening for Parkinson’s Disease with Response Time Barriers: A Pilot Study
Background: Although significant response time deficits (both reaction time and movement time) have been identified in numerous studies of patients with Parkinson’s disease (PD), few attempts have been made to evaluate the use of these measures in screening for PD.
Methods: Receiver operator characteristic curves were used to identify cutoff scores for a unitweighted composite of two choice response tasks in a sample of 40 patients and 40 healthy participants. These scores were then cross-validated in an independent sample of 20 patients and 20 healthy participants.
Results: The unit-weighted movement time composite demonstrated high sensitivity (90%) and specificity (90%) in the identification of PD. Movement time was also significantly correlated (r = 0.59, p \u3c 0.025) with the motor score of the Unified Parkinson’s Disease Rating Scale (UPDRS).
Conclusions: Measures of chronometric speed, assessed without the use of biomechanically complex movements, have a potential role in screening for PD. Furthermore, the significant correlation between movement time and UPDRS motor score suggests that movement time may be useful in the quantification of PD severity
Etiology, triggers and neurochemical circuits associated with unexpected, expected, and laboratory-induced panic attacks
Panic disorder (PD) is a severe anxiety disorder that is characterized by recurrent panic attacks (PA), which can be unexpected (uPA, i.e., no clear identifiable trigger) or expected (ePA). Panic typically involves an abrupt feeling of catastrophic fear or distress accompanied by physiological symptoms such as palpitations, racing heart, thermal sensations, and sweating. Recurrent uPA and ePA can also lead to agoraphobia, where subjects with PD avoid situations that were associated with PA. Here we will review recent developments in our understanding of PD, which includes discussions on: symptoms and signs associated with uPA and ePAs; Diagnosis of PD and the new DSM-V; biological etiology such as heritability and gene×environment and gene×hormonal development interactions; comparisons between laboratory and naturally occurring uPAs and ePAs; neurochemical systems that are associated with clinical PAs (e.g. gene associations; targets for triggering or treating PAs), adaptive fear and panic response concepts in the context of new NIH RDoc approach; and finally strengths and weaknesses of translational animal models of adaptive and pathological panic states
Investigating the effects of signal light position on human workload and reaction time in human-robot collaboration tasks
Critical to a seamless working relationship in human-robot collaborative environments is effective and frequent communication. This study looked to assess whether placing a light source on a robot was more effective for informing the human operator of the status of the robot than conventional human-machine interfaces for industrial system signaling such as light towers. Participants completed an assembly task while monitoring a robot and changes to the light sources: either from one of two light towers or LED strip lights attached to the robot. Workload was assessed by measuring reaction times to light changes and by counting number of completed assemblies. Although both the ANOVA and Friedman tests returned none significant results, total misses per condition showed that the participants did not miss any of the robot lights, whereas signals were missed for the light towers
- …