Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-2

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>er cell plating, 0.5 μM BIO was added to the culture medium. Fifteen days later, the number of colonies was scored. Bars represent means of 2 independent experiments. **: p < 0.01, ***: p < 0.00

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-3

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>bsence (Ctr) or presence of 0.5 μM BIO or 0.5 μM MeBIO. Ten days later, cells were stained with Oil Red O for adipocytes or with Alizarin red for osteoblasts (left panel). Effects of the compounds on GPDH, expressed in adipocytes only, and ALP, expressed in osteoblasts only, enzymatic activities are shown (right panel). Bars are the means ± SE of 3 independent experiments. *: p < 0.05, **: p < 0.0

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-4

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>sence of 0.5 μM BIO or 0.5 μM MeBIO. Ten days later, RNAs were prepared and expression of adipogenic or osteogenic markers was checked by qPCR. Results are the log2 inductions of BIO treated cells versus MeBIO treated cells. Expression data are colour-coded according to the scale

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-6

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>aled after treatment with 0.5 μM BIO or 0.5 μM MeBIO for 24 hours. DAPI was used to label nuclei (A). hMADS2 and hMADS3 cells were maintained as in (A) and fold induction in the expression of Gli1 gene was quantified by real-time PCR 24 hours after treatment with 0.5 μM BIO, 0.5 μM MeBio, 20 mM LiCl or 20 mM NaCl or under control condition (Ctr). Bars are the means ± S.E of 2 independent experiments, **: p < 0.01 (B)

Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes-0

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p>aled after treatment with 0.5 μM BIO or 0.5 μM MeBIO for 24 hours. DAPI was used to label nuclei (A). hMADS2 and hMADS3 cells were maintained as in (A) and fold induction in the expression of Gli1 gene was quantified by real-time PCR 24 hours after treatment with 0.5 μM BIO, 0.5 μM MeBio, 20 mM LiCl or 20 mM NaCl or under control condition (Ctr). Bars are the means ± S.E of 2 independent experiments, **: p < 0.01 (B)

HMADS3 cells were induced to undergo differentiation into the indicated lineages in the presence of BIO and MeBIO during the first 3 days

<p><b>Copyright information:</b></p><p>Taken from "Effects of GSK3 inhibitors on in vitro expansion and differentiation of human adipose-derived stem cells into adipocytes"</p><p>http://www.biomedcentral.com/1471-2121/9/11</p><p>BMC Cell Biology 2008;9():11-11.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2257931.</p><p></p> Then, compounds were withdrawn and GPDH and ALP enzymatic activities were quantified at day 10. *: p < 0.05, **: p < 0.01, NS: not significant (A). hMADS3 cells were induced to undergo differentiation into adipocytes (upper panel) or osteoblasts (lower panel) as in A) and RNAs were prepared at day ten. The expression of molecular markers was checked by qPCR (B). Results are the log2 inductions of BIO treated cells versus MeBIO treated cells. Expression data are colour-coded according to the scale that is displayed

Components of TGFβ-signaling pathway in human subcutaneous AT cells.

<p><i>TGFβ R1</i> (<i>ALK5</i>) (A), <i>activinA R1</i> (<i>ACVR1A/ALK2</i>) (B), <i>SMAD2</i> (C), <i>SMAD3</i> (D), <i>FIBRONECTIN</i> (E) and <i>PAI-1</i> (F) transcript levels were determined by real-time PCR in ScAT mature adipocytes (Adip), endothelial cells (EC), progenitor cells (Prog) and ATM. Values are means ± SEM (AU, arbitrary units) of 5 to 27 independent. * P<0.05, ** P<0.01 and *** P<0.001 between cell types.</p

AT location affects the remodeling phenotype of human AT macrophages in obese individuals.

<p>A, Transcript levels of angiogenic and matrix remodeling/fibrotic factors were determined by real-time PCR analyses of ATM immunoselected from paired biopsies of subcutaneous (Sc) and omental AT (Om). Results are expressed as fold differences between Om and Sc and are means ± SEM (n = 22 subjects, mean BMI 43.9±1.4 kg/m²). Open bars: genes up-regulated, and solid bars: genes down-regulated, in OmATM <i>vs</i> ScATM. B, Transcript levels of <i>HIF-1α</i> and <i>-2α</i> determined by real-time PCR analyses of ScATM and OmATM. Values are means ± SEM (AU, arbitrary unit) of the 22 paired biopsies. * P<0.05 and *** P<0.001 <i>vs</i> Om.</p

Obesity is associated with increased expression of myofibroblast markers in subcutaneous AT progenitor cells.

<p>A and B, Representative photomicrograph of immunohistochemistry of whole ScAT staining: α-SMA (red), CD34 (green) and nuclei (Hoechst 33242/blue) (n = 9). White scale corresponds to 50 µm. C, <i>SNAIL</i> and <i>SLUG</i> transcript levels were determined by real-time PCR in immunoselected ScAT progenitor cells from 7 non obese (Nob) and 8 obese (Ob) individuals. Values are means ± SEM (AU, arbitrary units). * P<0.05 and ** P<0.01 <i>vs</i> Nob.</p

TGFβ1 and subcutaneous AT macrophage-conditioned media induce α-SMA expression in human AT progenitor cells.

<p>Representative photomicrograph of immunocytochemical staining for α-SMA (red) of ScAT progenitors cells (n = 4) cultured for 48 h with basal medium (i.e, control), with TGFβ1 (5 ng/ml, n = 4, upper panel) or with ScATM-CM (n = 6, middle and lower panels). Nuclei were stained with Hoechst 33242 (blue). Magnification ×20 and ×40. White scale corresponds to 50 µm.</p