Phylogenetic analysis of GBoV1.

<p>(A) Full length structural protein (VP1/2) sequences of all HBoV and animal bocavirus strains available in GenBank were used to determine phylogeny of GBoV1 by neighbor-joining analysis of pairwise distances between translated amino acid sequences. Bootstrap re-sampling was used to determine robustness of individual clades (values above 70% shown above the branches). (B) The 4 major open reading frames of GBoV1 were analyzed using maximum likelihood composition analysis method (MEGA4.1) comparing pairwise distances of translated sequences of representative variants (reference sequence) of all four HBoV species. Accession numbers of sequences used precedes the name of corresponding bocavirus species.</p

Comparison of pairwise nucleotide and amino acid (bold typeface) distances (p-distance) of all four genes between GBoV1 and HBoV species confirms GBoV1 as prototype of a new bocavirus species.

<p>Comparison of pairwise nucleotide and amino acid (bold typeface) distances (p-distance) of all four genes between GBoV1 and HBoV species confirms GBoV1 as prototype of a new bocavirus species.</p

Phylogenetic relationship of GBV-D to other GBV and hepaciviruses.

<p>GBV-D amino acid sequences for A: NS5B, B: NS3, and C: the polyprotein (PPT) were analyzed in comparison to representative sequences of GBV-A, -B, -C and hepatitis C viruses. GenBank accession numbers for the respective sequences are indicated. Entebbe bat virus was used as an outgroup; distance in substitutions per site is indicated by scale bars; percent bootstrap support for values greater than 85% is indicated at respective nodes.</p

Percent sequence similarity between GBV-D (bat-68), -A, -C, and hepaciviruses.

<p>*Sequence similarity at nt level in upper right, and at aa level in lower left portion of the table.</p

Genomic organization of GBV-D, a novel flavivirus identified in the sera of frugivorous bats in Bangladesh.

<p>Arrows, glycosylation sites; solid diamond, active center sites H₉₂₁, E₁₀₁₁, and C₁₀₃₂ in the autocatalytic NS2/NS3 endoprotease domain; triangle, catalytic triad H₁₁₂₃, D₁₁₄₇, S₁₂₀₄ of NS3 serine protease; rectangle, NS3 helicase and DEAD-like helicase motifs; open diamond, zinc finger motif; and NS5 polymarase motifs A (T₂₇₄₄VDAICFDSCIT), B (R₂₈₀₂ASGVLTTSSSNCISSFLKVSAAC), C (F₂₈₃₅LIHGDDVMII), D (L₂₈₇₆DTAQSCSA),and E (H₂₉₀₀YFLSTDFR) motifs.</p

Geographic locations of Ebola HF outbreaks and phylogenetic relationships of representative filoviruses.

<p>(A) Map of Africa showing the sites of all known ebolavirus outbreaks denoted by colored circles for <i>Zaire ebolavirus</i> (yellow), <i>Sudan ebolavirus</i> (green), and <i>Côte d'Ivoire ebolavirus</i> (red). The expanded map of Uganda shows the location of the communities of Bundibugyo and Kikyo (black circles) in western Uganda, the site of the recent outbreak of <i>Bundibugyo ebolavirus</i>. Also shown on the Uganda map are the cities of Kampala (capital), Entebbe (international airport) and Gulu (the site of an outbreak of <i>Sudan ebolavirus</i> in 2000, the largest known Ebola HF outbreak on record). (B) Phylogenetic tree comparing full-length genomes of ebolavirus and marburgvirus by Bayesian analysis. Posterior probabilities greater than 0.5 and maximum likelihood bootstrap values greater than 50 are indicated at the nodes.</p

Additional file 1: of Pathosphere.org: pathogen detection and characterization through a web-based, open source informatics platform

(zip 23 kb