2 research outputs found
16S rRNA Gene-targeted TTGE in Determining Diversity of Gut Microbiota during Acute Diarrhoea and Convalescence
The human gut microbiota play a vital role in health and nutrition but
are greatly modified during severe diarrhoea due to purging and
pathogenic colonization. To understand the extent of loss during and
after diarrhoea, faecal samples collected from children (n=21)
suffering from acute diarrhoea and from their healthy siblings (n=9)
were analyzed by 16S rRNA gene-targeted universal primer polymerase
chain reaction (PCR), followed by temporal temperature gradient gel
electrophoresis (TTGE). The gut microbiota decreased significantly as
indicated by the number of TTGE bands at day 0 of acute diarrhoea
[patients vs healthy siblings: 11\ub10.9 vs 21.8\ub11.1
(mean\ub1standard error), p<0.01]. The number of bands showed a
steady increase from day 1 to day 7; however, it remained significantly
less than that in healthy siblings (15\ub10.9, p<0.01). These
results suggest that appropriate therapeutic and post-diarrhoeal
nutritional intervention might be beneficial for the early microbial
restoration and recovery
16S rRNA Gene-targeted TTGE in Determining Diversity of Gut Microbiota during Acute Diarrhoea and Convalescence
The human gut microbiota play a vital role in health and nutrition but
are greatly modified during severe diarrhoea due to purging and
pathogenic colonization. To understand the extent of loss during and
after diarrhoea, faecal samples collected from children (n=21)
suffering from acute diarrhoea and from their healthy siblings (n=9)
were analyzed by 16S rRNA gene-targeted universal primer polymerase
chain reaction (PCR), followed by temporal temperature gradient gel
electrophoresis (TTGE). The gut microbiota decreased significantly as
indicated by the number of TTGE bands at day 0 of acute diarrhoea
[patients vs healthy siblings: 11±0.9 vs 21.8±1.1
(mean±standard error), p<0.01]. The number of bands showed a
steady increase from day 1 to day 7; however, it remained significantly
less than that in healthy siblings (15±0.9, p<0.01). These
results suggest that appropriate therapeutic and post-diarrhoeal
nutritional intervention might be beneficial for the early microbial
restoration and recovery