5 research outputs found
The impacts of timber harvesting on residual trees and seedlings in a tropical rain forest ecosystem, southwestern Nigeria
- Author
- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes
- Author
- A Borgia
- A Hoffmann
- A Hoffmann
- A Soranno
- AN Kapanidis
- AS Kane
- B Schuler
- B Schuler
- B Schuler
- B Schuler
- Bengt Wunderlich
- Benjamin Schuler
- D Nettels
- D Nettels
- Daniel Nettels
- E Rhoades
- E Rhoades
- E Sherman
- E Sisamakis
- EA Lemke
- EA Lipman
- EV Kuzmenkina
- G Dimitriadis
- G Taylor
- GA Cooksey
- H Hofmann
- H Hofmann
- H Neuweiler
- H Roder
- Hagen Hofmann
- HS Chung
- HS Chung
- HS Chung
- IV Gopich
- JA Dunkle
- JB Knight
- JC McDonald
- Jennifer Clark
- JP Brody
- K Kawahara
- K Liu
- KM Hamadani
- M Gösch
- M Mueller
- M Pirchi
- N Eifler
- P Arora
- PS Dittrich
- PS Dittrich
- RA Marshall
- RD Smiley
- RK Benninger
- Sascha Weidner
- SH Pfeil
- Shawn H Pfeil
- Stephan Benke
- T Ha
- TJ Arbour
- WJ Greenleaf
- XW Zhuang
- Y Gambin
- Y Gambin
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2013
- Field of study
Microfluidic mixing in combination with single-molecule spectroscopy allows the investigation of complex biomolecular processes under non-equilibrium conditions. Here we present a protocol for building, installing and operating microfluidic mixing devices optimized for this purpose. The mixer is fabricated by replica molding with polydimethylsiloxane (PDMS), which allows the production of large numbers of devices at a low cost using a single microfabricated silicon mold. The design is based on hydrodynamic focusing combined with diffusive mixing and allows single-molecule kinetics to be recorded over five orders of magnitude in time, from 1 ms to ∼100 s. Owing to microfabricated particle filters incorporated in the inlet channels, the devices provide stable flow for many hours to days without channel blockage, which allows reliable collection of high-quality data. Modular design enables rapid exchange of samples and mixing devices, which are mounted in a specifically designed holder for use with a confocal microscopy detection system. Integrated Peltier elements provide temperature control from 4 to 37 °C. The protocol includes the fabrication of a silicon master, production of the microfluidic devices, instrumentation setup and data acquisition. Once a silicon master is available, devices can be produced and experiments started within ∼1 d of preparation. We demonstrate the performance of the system with single-molecule Förster resonance energy transfer (FRET) measurements of kinetics of protein folding and conformational changes. The dead time of 1 ms, as predicted from finite element calculations, was confirmed by the measurements
The assembly dynamics of the cytolytic pore toxin ClyA
- Author
- A Hoffmann
- A Ikai
- A Ludwig
- A Ortega
- AJ Wallace
- AP Capaldi
- AP Heuck
- B Schuler
- B Schuler
- B Wunderlich
- B Wunderlich
- BK Müller
- C Eggeling
- C Wong
- D Nettels
- D Nettels
- D Roderer
- DA Fancy
- E Sisamakis
- EF Pettersen
- EV Kuzmenkina
- F Rahimi
- FCO Los
- FG Vandergoot
- FJ del Castillo
- G Bitan
- G Lipari
- G Schreiber
- G Wildegger
- H Hofmann
- HX Zhou
- I Iacovache
- J Tschopp
- JH Ren
- JK Binder
- JM Zhao
- JR Thompson
- K Kuwajima
- KA Christensen
- L Song
- LJ Huang
- LO Tjernberg
- M Koshioka
- M Mueller
- M Soskine
- MD Hanwell
- MS Vaidyanathan
- MW Parker
- N Eifler
- N Poklar
- N Rojko
- OB Ptitsyn
- RP Rennie
- S Katen
- SB Tzokov
- SH Pfeil
- SN Wai
- T Dertinger
- T Vanaken
- TQ Zhang
- VT Nguyen
- Z Xie
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Spondylolisthesis
- Author
- A Grzegorzewski
- A Magora
- A Nematbakhsh
- A Ooij van
- A Ruiz-Cotorro
- A Shelekov
- A Wild
- AB King
- AH Crawford
- AH Crawford
- BE Fredrickson
- BM Cyron
- BM Cyron
- BW Mc Kee
- C Marty
- CJ Harvey
- CL Stanitski
- CN Pease
- CV Ward
- D Bell
- D Boxall
- D Lambl
- D Nordström
- D Schlenzka
- D Schlenzka
- D Schlenzka
- D Schlenzka
- DR Baker
- DS Bradford
- DS Bradford
- DS Hanson
- DS Hanson
- DW Jackson
- E Morscher
- E Pfeil
- E Pfeil
- EE Transfeld
- EM Lance
- EV Cheung
- F Hefti
- F Hefti
- F Sailhan
- FC Sherman
- GM Ivanic
- GS Phalen
- GV Johnson
- H Labelle
- H Saraste
- H Tiusanen
- H Zippel
- H Zippel
- HC Sagi
- HF Farfan
- HH Matthiass
- HL Shuffleberger
- HR McCarroll
- I Helenius
- I Helenius
- J Pfeil
- J Sys
- J Villiaumey
- J-M Mac-Thiong
- JA Smith
- JCT Fairbank
- JD King
- JE Buck
- JH Moe
- JK Burkus
- JL Beguiristáin
- JP Lubicky
- JP Lubicky
- JP O’Brien
- JR Johnson
- K Frennered
- K Fujii
- K Higashino
- K Kajiura
- K Sairyo
- K Sairyo
- K Sairyo
- K Österman
- K Österman
- KH Bridwell
- KL Wertzberger
- KP Schulitz
- L Virta
- LE Laurent
- LE Laurent
- LE Laurent
- LG Lenke
- LG Lenke
- LJ Grobler
- LJ Taylor
- LL Wiltse
- LL Wiltse
- LL Wiltse
- LL Wiltse
- LL Wiltse
- LL Wiltse
- LL Wiltse
- LM Belfi
- M Albanese
- M Kimura
- M Muschik
- M Muschik
- M Poussa
- M Poussa
- M Poussa
- M Ruf
- ME Steiner
- MJ Herman
- MR Rask
- N Boos
- NJ Rosenberg
- O Scaglietti
- P Bartolozzi
- P Gillet
- PA Basu
- PA d’Hemecourt
- PD Sponseller
- PG Marchetti
- PH Newman
- R Barrack
- R Cavalier
- R Louis
- R Vialle
- R Vialle
- R Wynne-Davies
- RB Winter
- RD Moreton
- RJ Bodner
- RL Carl
- RN Natarajan
- RO Nicol
- RP Huang
- RT Loder
- RW Gaines
- RW Gaines
- RW Molinari
- S Friberg
- S Ishikawa
- S Kleinberg
- S Komatsubara
- S Mardjetko
- S Seitsalo
- S Seitsalo
- S Seitsalo
- S Seitsalo
- S Seitsalo
- S Seitsalo
- S Seitsalo
- S Seitsalo
- SB Antoniades
- SD Lucey
- SE Borkow
- SF Miller
- SJ Kumar
- SM Lehmer
- T Lamberg
- T Sakamaki
- TD Steward
- TE Whitesides
- TR Haher
- TS Lamberg
- V Remes
- VM Remes
- W Lambl
- W Taillard
- WJ Beutler
- WJ Finnegan
- WT Dick
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2011
- Field of study
Anorganische Verbindungen mit einem Ion oder mehreren Ionen mit nicht abgeschlossenen Schalen (außer den Verbindungen in Abschnitt 29 2)
- Author
- A Arrott
- A Arrott
- A Arrott
- A Arrott
- A Baroni
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Bose
- A Burdese
- A Burdese
- A Burdese
- A Duperier
- A Duperier
- A Duperier
- A Duperier
- A Ferrai
- A Friederich
- A Hutchison Jr.
- A Merz
- A Merz
- A Michel
- A Michel
- A Mookherji
- A Mookherji
- A Mookherji
- A Mookherji
- A Mookherji
- A Nicolau
- A Okazaki
- A Osawa
- A Pabst
- A Pabst
- A Pabst
- A Rossi
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Serres
- A Westgren
- AA Buckmaster
- AE Florin
- AE Sanchez
- AE Sanchez
- AF Andresen
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
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- AH Cooke
- AH Cooke
- AH Cooke
- AH Cooke
- AI Karcheviskii
- AI Karchevskii
- AI Karchevskii
- AI Karchevskii
- AI Snow
- AI Snow
- AJ Kurushin
- AJP Meyer
- AJP Meyer
- AK Kikoin
- AM Hellwege
- AM Hellwege
- AM Hellwege
- AM Hellwege
- AM Hellwege
- AM Hellwege
- AN Holden
- AS Borovik-Romanov
- AS Borovik-Romanov
- AS Borovik-Romanov
- AS Borovik-Romanov
- AS Borovik-Romanov
- AS Borovik-Romanow
- AS Wilson
- AW Nolle
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
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- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
- B Bleaney
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- B Cabrera
- B Cabrera
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- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
- B Cabrera
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- B Cabrera
- B Cabrera
- B Cabrera
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- CA Hutchison Jr.
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- CB Ellis
- CC Stephenson
- CD Adam
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- CL Burdick
- CM Nelson
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- E Ambler
- E Ambler
- E Carlson
- E Friedlich
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- E Lehrer
- E Pouillard
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- L Castellitz
- L Castellitz
- L Castellitz
- L Corliss
- L Heaton
- L Helmholtz
- L Howe
- L Howe
- L Néel
- L Néel
- L Néel
- L Passerini
- L Pauling
- L Pauling
- L Pauling
- L Pauling
- L Sacconi
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LC Jackson
- LD Roberts
- LD Roberts
- LD Roberts
- LD Roberts
- LD Roberts
- LD Roberts
- LF Bates
- LF Bates
- LF Bates
- LF Bates
- LF Bates
- LM Kovba
- LM Matarrese
- LR Maxwell
- LR Maxwell
- LR Maxwell
- LS Ramsdell
- LT Reynolds
- M Abraham
- M Abraham
- M Calvin
- M Date
- M Date
- M Griffel
- M Hansen
- M Hansen
- M Hansen
- M Kotani
- M Kotani
- M Laffite
- M Lindquest
- M Picon
- M Picon
- M Picon
- M Picon
- M Picon
- M Picon
- M Picon
- M Picon
- M Picon
- M Pion
- M Seguin
- M Stackeiberg
- M Stackelberg
- M Theodorides
- M Weger
- M Yamamoto
- M Yuzuri
- M Yuzuri
- M Yuzuri
- M Yuzuri
- M Yuzuri
- M Zieger
- MCM O’Brien
- ME Nicholson
- MG Inghram
- MH Hebb
- MH Mueller
- Milligan
- MJ Besnus
- MJ Buerger
- MJ Buerger
- MJ Steenland
- MK Wilkinson
- MK Wilkinson
- N Alsen
- N Alsen
- N Elliot
- N Elliott
- N Elliott
- N Elliott
- N Goldenberg
- N Goldenberg
- N Goldenberg
- N Goldenberg
- N Kurti
- N Kurti
- N Kurti
- N Kurti
- N Kürti
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Perakis
- N Uryu
- N Wooster
- NC Baenziger
- NH Kolkmeijier
- NJ Hawkins
- NK Abrikosov
- NK Abrikosov
- NK Abrikosov
- NN Zhuravley
- NP Grarhdarkina
- NP Grazhdankina
- NS Garifyanow
- NS Gingrich
- O Arstad
- O Eisenhut
- O Hassel
- O Trapenznikowa
- OE Frivold
- P Blum
- P Chiotti
- P Duwez
- P Ehrlich
- P Ehrlich
- P Fields
- P Gordon
- P Graf
- P Grot
- P Groth
- P Groth
- P Handler
- P Handler
- P Hausknecht
- P Hausknecht
- P Hausknecht
- P Henkel
- P Khodadad
- P Khodadad
- P Khodadad
- P Khodadad
- P Kierkegaard
- P Lehmann
- P Lesage-Bourdon
- P Weiss
- P Weiss
- P Weiss
- P Weiss
- PA Sellers
- PB Dorain
- PCL Pfeil
- PF Kerr
- PL Mukherjee
- PL Mukherjee
- PP Ewald
- PP Ewald
- PR Ray
- PR Ray
- PW Anderson
- PW Selwood
- PW Selwood
- PW Selwood
- PW Selwood
- PW Selwood
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Benoit
- R Bridelle
- R Bridelle
- R Bridelle
- R Brill
- R Caillât
- R Chevallier
- R Finkelstein
- R Gross
- R Hocart
- R Hoppe
- R Hoppe
- R Hoppe
- R Hoppe
- R Juza
- R Juza
- R Juza
- R Juza
- R Juza
- R Kiefer
- R Lindsey
- R Lindsey
- R Mooney
- R Nast
- R Pauthenet
- R Stoenner
- R Ueda
- RA Alikhanov
- RA Alikhanov
- RA Erickson
- RA Erickson
- RA Fereday
- RA Fereday
- RA Fereday
- RA Fereday
- RA Satten
- RC Shaney
- RC Vickery
- RC Vickery
- RC Vickery
- RC Vickery
- RCL Mooney
- RE Rundle
- RE Rundle
- RE Rundle
- RE Rundle
- RE Rundle
- RE Rundle
- RE Rundle
- RG Butters
- RG Loasby
- RJ Benzie
- RJ Benzie
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RJ Elliott
- RM Bozorth
- RM Bozorth
- RM Bozorth
- RM Bozorth
- RM Bozorth
- RM Douglass
- RNR Mulford
- RNR Mulford
- RNR Mulford
- RP Hudson
- RR Heikes
- RR Heikes
- RS Roth
- RS Trenam
- RT Weiner
- RW Asmussen
- RW Asmussen
- RW Hill
- RW Kedzie
- RW Lawrence
- RWG Wyckoff
- RWG Wyckoff
- RWG Wyckoff
- S Chiba
- S Chiba
- S Freed
- S Freed
- S Freed
- S Freed
- S Freed
- S Freed
- S Freed
- S Greenwald
- S Kaya
- S Kume
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Meyer
- S Ramaseshan
- S Sidhu
- S Sidhu
- S Siegel
- S Siegel
- S Steingiser
- S Sugden
- S Sugden
- S Tengnér
- S Tengnér
- S Tengnér
- S Uyeda
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- S Velayos
- SA Altshuler
- SB Hendricks
- SF Malaker
- SG Salikhov
- SH Bauer
- SH Bogle
- SH Yu
- SJ Leach
- SJ Picart
- SJ Pickart
- SN Ghosh
- SS Bahtnager
- ST Lin
- ST Lin
- ST Lin
- SW Rabideau
- T Haseda
- T Haseda
- T Haseda
- T Haseda
- T Hihara
- T Hirone
- T Hirone
- T Hirone
- T Hirone
- T Hirone
- T Hirone
- T Hirone
- T Ishiwara
- T Iwata
- T Karantassis
- T Karantassis
- T Karantassis
- T Karantassis
- T Mhirone
- T Murao
- T Nagata
- T Nagata
- T Nagata
- T Nagata
- T Ohtsuka
- T Otsuka
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Rosenqvist
- T Suzuoka
- T Suzuoka
- T Suzuoka
- T Townsend Smith
- T Watanabe
- TD Zotov
- TG Burke
- TH Geballe
- TI Sanadze
- TI Sanadze
- TJ Heal
- TJ Swoboda
- TJ Swoboda
- U Fürst
- U Johnsen
- U Johnsen
- U Zwicker
- V Cagliosti
- V Cirilli
- V Gutmann
- V Van
- V Vleck
- VE Frivold
- VG Paranjpe
- VI Barkhatov
- VI Barkhatov
- VI Barkhatov
- VY Kurenev
- W Biltz
- W Biltz
- W Biltz
- W Biltz
- W Biltz
- W Blitz
- W Blitz
- W Doll
- W Hofmann
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Klemm
- W Low
- W Menzer
- W Palmer
- W Pfeffer
- W Pfeffer
- W Pfeffer
- W Rudorff
- W Rudorff
- W Rudorff
- W Rudorff
- W Rüdorf f
- W Rüdorff
- W Rüdorff
- W Rüdorff
- W Sucksmith
- W Sucksmith
- W Sucksmith
- W Sucksmith
- W Sucksmith
- W Sucksmith
- W Sucksmith
- W Tilk
- W Trzebiatowski
- W Trzebiatowski
- W Trzebiatowski
- W Trzebiatowski
- W Trzebiatowski
- W Trzebiatowski
- W Trzebiatowski
- W Zachariasen
- W Zachariasen
- WB Lewis
- WB Lewis
- WC Koehler
- WC Koehler
- WC Koehler
- WC Koehler
- WE Gardner
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WE Henry
- WF Giauque
- WF Giauque
- WF Giauque
- WF Giauque
- WF Hong de
- WF Jong de
- WF Jong de
- WF Jong de
- WF Jong de
- WG Burgers
- WG Burgers
- WH Cloud
- WH Cloud
- WH Henry
- WH Henry
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WH Zachariasen
- WJ Haas de
- WJ Haas de
- WJ Haas de
- WJ Haas de
- WJ Haas de
- WJ James
- WJ Takei
- WL Bragg
- WL Korst
- WM Jones
- WM Jones
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- WN Hansen
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Ishikawa
- Y Kojima
- Y Shimomura
- Y Ting
- Y Ting
- Y Ting
- Y Ting
- Y Ting
- Y Yokozawa
- Y Yokozawa
- YK Chow
- YL Yousef
- Z Nishiyama
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2013
- Field of study