RNA content in motor and sensory neurons and surrounding neuroglia of mouse spinal cord under conditions of hypodynamia and following normalization

The differences in the dynamics of reparative processes in RNA metabolism within the neuron-neuroglia unit after the cessation of hyper- and hypodynamia is dicussed. The role of neuroglia is stressed in compensatory, reparative and trophic processes in the nervous system as well as the possibility in an adaptation at the cellular level

RNA content in motor and sensory neurons and surrounding neuroglia of mouse spinal cord under conditions of hypodynamia and following normalization

Male white mice were subjected to two and three week hypodynamia and then decapitated. Cytoplasmic RNA content per cell was measured by means of ultraviolet cytospectrometry. Changes in RNA content are shown, and the dynamics of the reparative processes of cells are discussed

Processing of land 4D seismic data in case of limited area of repeated survey - a case study from Otway Basin, Australia

Time lapse 3D seismic is an important part of monitoring and verification program of the Otway Project - an Australian first demonstration of the deep geological storage of CO2 located on-shore in Victoria. From March, 2008 CO2-rich gas is being injected into a depleted gas reservoir at a depth of around 2050 metres. In year 2000 3D pre-production seismic data were acquired over large area, covering Naylor field, our CO2 sequestration site and also several adjacent small gas fields. Baseline 3D seismic data were acquired in December 2007, however due to logistical problems and cost of the survey size of the area was much smaller (only 3 sq. km). Processing of land time-lapse seismic surveys is a challenging task and in this case it was additionally complicated since limited migration aperture was determined to be one of main problems affecting imaging of the target horizon. To overcome this limitation we adopted an approach which is based on joint processing of two different 3D vintage sets with different size of survey area

Topochemical differences in the amount of RNA in the motoneurons of the spinal chord in hypoxia and hypokinesia

Reactions to hypoxia and hypoknesia were compared by measuring charges in the amount of ribonucleic acid (RNA) in the cytoplasm of neurons of the intumescentia cervicalis and lumbalis. Animals were subjected to hypoxia, hypokinesia and both combined and a control group to neither. A total of two groups of motoneurons were compared, one innervating the respiratory musculature, the other the musculature of the lower extremities, so that hypoxic hypoxia would probably affect the first group primarily and hypokinesia the second. Results indicate that neither affect the amount of RNA in the neurons of the first group but a significant increase is noted in neurons of the second group. Other significant results are reported

Seismic Monitoring of CO2 Geosequestration in Otway Basin, Australia

CO2CRC Otway Project is the Australia s first demonstration of the deep geological storage of CO2. CO2 has been injected in a depleted gas field at the depth of 2050 m and then will be injected in saline aquifer at the depth of around 1 km. For time lapse studies, we had four different 3D seismic surveys available. Besides of a large 3D seismic volume acquired in the year 2000 prior gas production of the reservoir, three sequential 3D surveys were acquired at the same site but over a smaller area: baseline survey in 2008 and two monitoring surveys in 2009 and 2010. We concentrated on repeatability of 4D seismic data acquisition and processing. This led us to the results which allow to define CO2 location in the reservoir and proves that time-lapse seismic is a valuable tool in CO2 monitoring even in on-shore case

Repeatability of land time-lapse seismic surveys - Otway project 2D test line case study

Full range of 2D and 3D borehole and surface seismic methods are utilised for time-lapse surveys for monitoring of CO2 sequestration at Otway Basin pilot project site. Conducting land time-lapse surveys is challenging task because of typical variability of ground conditions, source-receiver coupling, ambient noise which results in poor repeatability of land seismic data. We analysed the major factors influencing repeatability of land seismic data. We also analysed both synthetic and field data for possible limitations of the surface reflection seismic method when it comes to repeatability. We show that changes in near surface conditions will produce kinematic differences but also different ground roll patterns. This will in general require slightly different parameters for processing of two successive surveys if they are acquired for vastly different soil conditions. We then conduct extensive numerical and field tests to show that the S/N variability as function of the source strength relative to the background noise level is crucial. Source type is less important for time lapse surveys as long as S/N ratio is high. Our tests included impact (weight drop) and vibrating (IVI Mini-Buggy) sources