МОРФОЛОГИЯ СПЕРМАТОЗОИДОВ КОТОВ РАЗЛИЧНЫХ ПОРОД

Morphological and physiological evaluation of spermatozoa is commonly used in the analysis of male semen. We found the lowest concentration of germ cells in Bengal cats, which is 100.6 million/ml less (P<0.05) from British breed cats, 227.49 million/ml less (P<0.001) from Maine Coon breed, 84.5 million/ml less (P<0.05) from cats of the Siberian breed and 152.16 million/ml less (P<0.01) from the Sphynx breed.Морфологическая и физиологическая оценка сперматозоидов обычно используется при анализе спермы самцов. Наименьшая концентрация половых клеток установлена нами у котов Бенгальской породы, что на 100,6 млн/мл меньше (Р<0,05) от котов Британской породы, на 227,49 млн/мл меньше (Р<0,001) от породы Мейн-кун, на 84,5 млн/мл меньше (Р<0,05) от котов Сибирской породы и на 152,16 млн/мл меньше (Р<0,01) от породы Сфинкс

Diagnostics of erythrocytes' microrheological features and early abnormalities of rats in the model of experimental hypertension development

Difficulties in the early stage detection of erythrocytes' microrheological abnormalities during the development of hypertension are connected with falling out of clinicians' opinion of persons with first signs of this pathology. It dictates the necessity of experimental investigations' fulfillment on laboratory animals with just developed hypertension in them. Eighty-seven of healthy male rats of Wistar line at the age of 2.5-3 months were taken into the investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. Fifty-eight rats had hypertension developed by prescribing them cardio angionefo pathogenic semisynthetic diet. For the purpose of investigation biochemical, hematological, and statistical methods were used. As a result of hypertension, the rats turned out to have developed an increase of systolic and diastolic pressure. At regular exercise, on the treadmill, the rats were noted to have a gradual decrease of their values during 60 days of investigation to the normal level. During hypertension development lipids' peroxidation activated in rats' erythrocytes because the activity of their antioxidant protection weakened. When hypertension occurred in rats the erythrocytes-discocytes quantity in blood was found to have decreased. It was accompanied by an increase of reversibly and irreversibly changed erythrocytes quantity in the examined animals' blood. When hypertension occurred in rats a quick rise of erythrocytes' sum in aggregate was found and the rise in these aggregates' quantity was due to lowering of free erythrocytes' number. During experimental hypertension modeling, we noticed that there was a very early decrease in the quantity of erythrocytes-discocytes in the rat blood, and their level raised reversibly and irreversibly with the strengthening of their aggregative ability. It happened because of the weakening of erythrocytes' antioxidant protection, and activation of lipids' peroxidation in them

Diagnostics of erythrocytes' microrheological features and early abnormalities of rats in the model of experimental hypertension development

Difficulties in the early stage detection of erythrocytes' microrheological abnormalities during the development of hypertension are connected with falling out of clinicians' opinion of persons with first signs of this pathology. It dictates the necessity of experimental investigations' fulfillment on laboratory animals with just developed hypertension in them. Eighty-seven of healthy male rats of Wistar line at the age of 2.5-3 months were taken into the investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. Fifty-eight rats had hypertension developed by prescribing them cardio angionefo pathogenic semisynthetic diet. For the purpose of investigation biochemical, hematological, and statistical methods were used. As a result of hypertension, the rats turned out to have developed an increase of systolic and diastolic pressure. At regular exercise, on the treadmill, the rats were noted to have a gradual decrease of their values during 60 days of investigation to the normal level. During hypertension development lipids' peroxidation activated in rats' erythrocytes because the activity of their antioxidant protection weakened. When hypertension occurred in rats the erythrocytes-discocytes quantity in blood was found to have decreased. It was accompanied by an increase of reversibly and irreversibly changed erythrocytes quantity in the examined animals' blood. When hypertension occurred in rats a quick rise of erythrocytes' sum in aggregate was found and the rise in these aggregates' quantity was due to lowering of free erythrocytes' number. During experimental hypertension modeling, we noticed that there was a very early decrease in the quantity of erythrocytes-discocytes in the rat blood, and their level raised reversibly and irreversibly with the strengthening of their aggregative ability. It happened because of the weakening of erythrocytes' antioxidant protection, and activation of lipids' peroxidation in them

Диагностика микрореологических особенностей эритроцитов и ранних аномалий развития экспериментальной гипертензии у крыс

Difficulties in the early stage detection of erythrocytes’ microrheological abnormalities during the development of hypertension are connected with falling out of clinicians’ opinion of persons with first signs of this pathology. It dictates the necessity of experimental investigations’ fulfillment on laboratory animals with just developed hypertension in them. Eighty-seven of healthy male rats of Wistar line at the age of 2.5–3 months were taken into the investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. Fifty-eight rats had hypertension developed by prescribing them cardio angionefo pathogenic semisynthetic diet. For the purpose of investigation biochemical, hematological, and statistical methods were used. As a result of hypertension, the rats turned out to have developed an increase of systolic and diastolic pressure. At regular exercise, on the treadmill, the rats were noted to have a gradual decrease of their values during 60 days of investigation to the normal level. During hypertension development lipids’ peroxidation activated in rats’ erythrocytes because the activity of their antioxidant protection weakened. When hypertension occurred in rats the erythrocytes-discocytes quantity in blood was found to have decreased. It was accompanied by an increase of reversibly and irreversibly changed erythrocytes quantity in the examined animals’ blood. When hypertension occurred in rats a quick rise of erythrocytes’ sum in aggregate was found and the rise in these aggregates’ quantity was due to lowering of free erythrocytes’ number. During experimental hypertension modeling, we noticed that there was a very early decrease in the quantity of erythrocytes-discocytes in the rat blood, and their level raised reversibly and irreversibly with the strengthening of their aggregative ability. It happened because of the weakening of erythrocytes’ antioxidant protection, and activation of lipids’ peroxidation in them.Сложности в выявлении микрореологических нарушений эритроцитов на ранних стадиях развития гипертонии связаны с тем, что клиницисты высказывают мнение о людях с первыми признаками этой патологии. Это диктует необходимость проведения экспериментальных исследований на лабораторных животных с только что развившейся гипертонией. Восемьдесят семь здоровых самцов крыс линии Вистар в возрасте 2,5–3 месяцев были включены в исследование. Двадцать девять животных из них не подвергались ударам и составили контрольную группу. У пятидесяти восьми крыс развилась артериальная гипертензия при назначении им патогенной полусинтетической диеты. Для исследования использовались биохимические, гематологические и статистические методы. В результате гипертонии у крыс развилось повышение систолического и диастолического давления. При регулярных тренировках на беговой дорожке у крыс отмечалось постепенное снижение их значений в течение 60 дней исследования до нормального уровня. Во время развития гипертонии перекисное окисление липидов активируется в эритроцитах крыс, потому что активность их антиоксидантной защиты ослабевает. При гипертонии у крыс количество эритроцитов-дискоцитов в крови уменьшилось. Это сопровождалось увеличением количества обратимо и необратимо измененных эритроцитов в крови исследуемых животных. Когда у крыс возникла гипертония, было обнаружено быстрое увеличение суммы эритроцитов в совокупности, и увеличение количества этих агрегатов было связано с уменьшением количества свободных эритроцитов. Во время экспериментального моделирования гипертонии мы заметили, что было очень раннее уменьшение количества эритроцитов-дискоцитов в крови крысы, и их уровень повышался обратимо и необратимо с усилением их агрегационной способности

Diagnostics of early dysfunctions of anticoagulant and fibrinolytic features of rats' vessels in the course of metabolic syndrome formation with the help of fructose model

The optimal feature of anticoagulant and fibrinolytic of the vascular wall provides the major homeostasis process in the whole body of a mammal. It is impossible to track in clinic peculiarities of the earliest change in vascular hemostasis in a debut of metabolic syndrome formation. Thus, it dictates the necessity of conducting experimental researches on laboratory animals with modeling of metabolic syndrome within them. The aim of the research is to examine early stages of vascular hemocoagulation weakening control in conditions such as the formation of metabolic syndrome. The study used 61 male-rats of Wister line at the age of 2.5-3 months. The animals were subdivided into two groups: 32 rats received 10% fructose dilution with free access for drinking, and 29 rats to the control group. Biochemical, hematological and statistical methods of investigation were applied. We found that in high fructose level, there is a fast evident of a weakening of the anticoagulant and fibrinolytic activities of vascular endothelium as well as the body mass gain and development of biochemical abnormalities, which is typical for metabolic syndrome. The early weakening of vascular control over hemocoagulation turns metabolic syndrome into a very dangerous state because of thromboses, which may take place even at the very beginning of its development