<i>R. aegyptiacus</i> colony maintained in large flight cage allowing for housing 150–200 bats.

<p><i>R. aegyptiacus</i> colony maintained in large flight cage allowing for housing 150–200 bats.</p

PCR and virus isolation results in <i>R. aegyptiacus</i> bats inoculated subcutaneously and intraperitoneally with Hogan strain of MARV.

<p>PCR  =  PCR results.</p><p>VI  =  Virus isolation results.</p><p>(/)  =  Values in brackets indicate number of positive bats per number of animal tested at a specific time point by PCR and VI.</p><p>n.s.  =  Not sampled.</p

PCR and virus isolation results in <i>R. aegyptiacus</i> bats inoculated subcutaneously with Hogan strain of MARV.

<p>PCR  =  PCR results.</p><p>VI  =  virus isolation results.</p><p>(/)  =  values in brackets indicate number of positive bats per number of animal tested at a specific time point by PCR and VI.</p><p>n.s.  =  not sampled.</p

IgG responses in <i>R. aegyptiacus</i> bats inoculated with MARV isolate MHK.

<p>Individual responses for adult females (•), adult males (○) and pups (x), and mean responses for females (----------), males (----------) and pups (•••) are shown. The ELISA cut-off value is indicated as a dashed line (---).</p

Experimental design and sample collection schedule in <i>R. aegyptiacus</i> bats inoculated with Hogan strain of MARV.

<p>a =  intraperitoneal.</p><p>b =  subcutaneous.</p>†<p> =  lethal sampling.</p><p># =  non-lethal sampling.</p>*<p> =  three remaining bats from group A were later used as group C for s.c. inoculation.</p

Layout of the SA FEDL in Freetown-Lakka, Sierra Leone with emergency generator and wiring to allow for rapid switch to generator mode in case of power failure.

<p>(<b>A</b>) Biocontainment negative pressure chamber (IsoArk), (<b>B</b>) Room housing biocontainment negative pressure chamber, (<b>C</b>) Donning room, (<b>D</b>) Doffing room, (<b>E</b>) Laboratory airlock area, (<b>F</b>) PCR amplification room, (<b>G</b>) PCR master mix room, (<b>H</b>) Specimens and reagents storage area, (<b>I</b>) RNA extraction room, (<b>J</b>) Facility entrance, (<b>K</b>) Toilet, (<b>L</b>) Office 1, (<b>M</b>) Office 2, (<b>N</b>) Office 3. Petrol generator (5.5 kVa) placement indicated by the red rectangle, distribution of extension cords are indicated with red lines, and emergency connection points by red stars.</p

Number of specimens from EVD suspected cases in Sierra Leone tested daily (total blood and buccal swabs) by SA FEDL during the first weeks of operation, 25 August—30 Sept 2014.

<p>Column = Number of specimens tested daily; Dotted line = Trend line of a number of specimens tested; Solid line = Maximum testing capacity of 58 specimens per day.</p

Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.

<p>Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.</p