СОЦІО-ЕКОНОМІЧНІ АСПЕКТИ ОРГАНІЗАЦІЇ«РОЗУМНОГО МІСТА»

<p>Breteau Index represents number of positive containers per 100 houses inspected. Stars indicate surveys with nil detection.</p

Maximum likelihood tree comparing DENV-1 complete E gene sequences (1485 base pairs).

<p>Strain names and respective genotypes are given including locally transmitted viruses (Townsville, Cairns, and Mareeba). GenBank accession numbers of retrieved global strains are shown in brackets. Bootstrap support values derived from 1,000 replicate NJ trees are represented for principal nodes >70%. DENV-2 strain Townsville 2004 was used as an outgroup control.</p

Local area of the case's workplace.

<p>Shown are: the case's workplace (cross in circle), containerised cargo delivery locations (dash in circle), apron boundaries (dashed line), southern airport boundary (dot and dash line), airplane arrival areas (airplane symbol), routine adult mosquito traps (red circle), and extra case adult mosquito traps (green circle). Image supplied courtesy of Northern Territory Government.</p

Mean (+SE) sweep-net collections of <i>Ae</i>. <i>albopictus</i> adults obtained from selected sampling sites on Horn, Thursday and Hammond islands at various times between 2012 and 2015.

<p>There were no vector control activities on Hammond Island. Previous survey on Hammond had been conducted in 2009 and did not detect any <i>Ae</i>. <i>albopictus</i>.</p

Harborage spraying using a vehicle-mounted unit to control <i>Ae</i>. <i>albopictus</i> on Thursday Island.

<p>Harborage spraying using a vehicle-mounted unit to control <i>Ae</i>. <i>albopictus</i> on Thursday Island.</p

Mean sweep-net collections of <i>Ae</i>. <i>albopictus</i> adults on Thursday Island 2011–2016.

<p>Periodic five-minute collections were conducted on up to 150 selected sampling sites. Stars indicate surveys with nil detection.</p

Prevalence of containers with <i>Ae</i>. <i>aegypti</i> larvae on Thursday Island for the period 2009–2016.

<p>Prevalence of containers with <i>Ae</i>. <i>aegypti</i> larvae on Thursday Island for the period 2009–2016.</p

A New Species of Mesonivirus from the Northern Territory, Australia

<div><p>Here we describe Casuarina virus (CASV), a new virus in the family <i>Mesoniviridae</i>. This is the first report of a mesonivirus in Australia, which extends the geographical range of this virus family to 3 continents. The virus was isolated in 2010 from <i>Coquillettidia xanthogaster</i> mosquitoes during surveillance in the suburbs of Darwin, the capital of the Northern Territory. Cryo-electron microscopy of the CASV virions revealed spherical particles of 65 nm in size with large club-shaped projections of approximately 15 nm in length. The new virus was most closely related to <i>Alphamesonivirus 1</i>, the only currently recognized species in the family. In 2013 a further 5 putative new mesonivirus species were described: Hana, Méno, Nsé, Moumo and Dak Nong viruses. The evolutionary distance between CASV and two of its closest relatives, Cavally and Hana viruses (Jones-Taylor-Thornton distance of 0.151 and 0.224, respectively), along with its isolation from a different genus of mosquitoes captured on a separate continent indicate that CASV is a new species.</p></div

Analysis for mesonivirus core using NP-40 treatment.

<p>Gradient-purified CASV particles were left untreated (A) or treated with 0.05% NP-40 for 6 min (B) or 0.25% NP-40 for 30 min (C) to disrupt the lipid envelope. No proteainaceous internal component was released suggesting the absence of a core. An uncoating virion is highlighted in panel B with an arrow. Scale bars: A = 500 nm, B and C = 200 nm.</p

Phylogenetic analysis of the replicase domains of pp1ab.

<p>Unrooted phylogenies were generated from the protein sequences of the indicated replicase domains using the maximum likelihood method. Bootstrapping replicates (1000) are rounded to a value out of 100. The branch length is proportional to the evolutionary distance as shown by the scale bar (substitutions per amino acid site).</p