12 research outputs found
Cell cycle analysis examined using flow cytometry on HT-29 cells after 72 h treatment.
<p>(A) Cells treated with (a) DMSO at the final concentration of 0.1%. (b) FKB at a concentration of 12.5 (3.55 μg/mL), (c) 25 (7.1 μg/mL), (d) 50 μM (14.2 μg/mL), and (e) Percentage of cell cycle distribution in different phases. (B) Cells treated with (a) DMSO at the final concentration of 0.1%. (b) APN at 12.5 (3.37 μg/mL), (c) 25 (6.75 μg/mL), (d) 50 μM (13.5 μg/mL) concentrations, and (e) Percentage of cell cycle distribution in different phases. G0/G1, G2+M, and S are cell phases, respectively; subG0/G1 refers to cell death due to DNA fragmentation. Data are expressed as Mean±SD of three independent experiments, *p<0.001, ns: non-significant compared to the normal control.</p
DNA fragmentation analysed in 1% agarose gel after 72 h incubation with different concentration of either FKB or APN.
<p>MW: DNA marker; DC: DMSO treated control; all concentrations are in μM.</p
Levels of MDM2 and p53 proteins expressed in HT-29 cells.
<p>(A) Level of proteins in cells treated with (a) crude hexane (IC<sub>25</sub>: 10.52, IC<sub>50</sub>: 21.05, and IC<sub>75</sub>:42.1 μg/mL) and chloroform (IC<sub>25</sub>: 9.5, IC<sub>50</sub>: 19.09, and IC<sub>75</sub>:38.18 μg/mL) extracts. (b) 25 μM (7.1 μg/ mL) of FKB at different time interval. (c) 25 μM (6.75 μg/mL) of APN at different time interval. (B) Level of MDM2 and p53 protein expression quantified from western blotting analysis using Bio-rad Image Lab software in HT 29 cells treated with (a) Hexane and chloroform extracts (b) FKB, and (c) APN. Data are expressed as Mean±SD; ns: non-significant; *p<0.05; **p<0.01; ***p<0.01; ns: non-significant compared to the DMSO control. DC: DMSO used as negative control at a final concentration of 0.1%.</p
DNA fragmentation of MDA-MB 231 breast cancer cells analysed in 1% agarose gel after 24 hours incubation with different concentration of Artonin E.
<p>Lanes 1–3 represents 3, 10 and 30 μM of Artonin E, lane 4 is untreated cancer cells, lane 5 is positive control well treated with 4μg/mL camptothecin and lane 6 is a 1kilobase DNA ladder.</p
Acridine orange and propidium iodide double staining of MDA-MB 231 breast cancer cells after 24 hour exposure.
<p>(A) Control, (B) 3 μM Artonin E. (C) 10 μM Artonin E, (D) 30 μM Artonin E and (E) Quantification of apoptotic morphology at 24 and 48 hours. Each result is presented as mean ± SD of three replicates. * indicates significant difference from the control of each phase (p<0.05). VC = Viable cells; BL = Cell membrane blebbing; CC = chromatin condensation; EA = Early apoptosis; LA = late apoptosis; MN = marginated nuclear chromatin; SN = secondary necrosis. Magnification X200.</p
The percentage of MDA-MB-231 cell line viability after treatment with Artonin E.
<p>The experiment was done in triplicate at 24, 48, and 72 hours and each point are presented as mean ± SD.</p
The accession number and sequence of the primers used in the gene expression studies.
<p>The accession number and sequence of the primers used in the gene expression studies.</p
Mechanism of action of Artonin E in MDA-MB 231 breast cancer cells.
<p>Mechanism of action of Artonin E in MDA-MB 231 breast cancer cells.</p
Western blot analysis of Artonin E (3, 10, and 30 μM) treated MDA-MB231 breast cancer cells after 24 hours.
<p>(A) and the levels of protein expression quantified from the western blotting analysis of Artonin E treated MDA-MB 231 cells using Bio-rad Image Lab software (B).</p