Insights into antibody catalysis: Structure of an oxygenation catalyst at 1.9-Å resolution

The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-Å and 1.9-Å resolution, respectively. To our knowledge, these structures are the highest resolution catalytic antibody structures to date and provide insight into the molecular mechanism of this antibody-catalyzed monooxygenation reaction. Specifically, the data suggest that entropic restriction plays a fundamental role in catalysis through the precise alignment of the thioether substrate and oxidant. The antibody active site also stabilizes developing charge on both sulfur and periodate in the transition state via cation-pi and electrostatic interactions, respectively. In addition to demonstrating that the active site of antibody 28B4 does indeed reflect the mechanistic information programmed in the aminophosphonic acid hapten, these high-resolution structures provide a basis for enhancing turnover rates through mutagenesis and improved hapten design

Synchronous wearable wireless body sensor network composed of autonomous textile nodes

A novel, fully-autonomous, wearable, wireless sensor network is presented, where each flexible textile node performs cooperative synchronous acquisition and distributed event detection. Computationally efficient situational-awareness algorithms are implemented on the low-power microcontroller present on each flexible node. The detected events are wirelessly transmitted to a base station, directly, as well as forwarded by other on-body nodes. For each node, a dual-polarized textile patch antenna serves as a platform for the flexible electronic circuitry. Therefore, the system is particularly suitable for comfortable and unobtrusive integration into garments. In the meantime, polarization diversity can be exploited to improve the reliability and energy-efficiency of the wireless transmission. Extensive experiments in realistic conditions have demonstrated that this new autonomous, body-centric, textile-antenna, wireless sensor network is able to correctly detect different operating conditions of a firefighter during an intervention. By relying on four network nodes integrated into the protective garment, this functionality is implemented locally, on the body, and in real time. In addition, the received sensor data are reliably transferred to a central access point at the command post, for more detailed and more comprehensive real-time visualization. This information provides coordinators and commanders with situational awareness of the entire rescue operation. A statistical analysis of measured on-body node-to-node, as well as off-body person-to-person channels is included, confirming the reliability of the communication system

Flexible dual-diversity wearable wireless node integrated on a dual-polarised textile patch antenna

A new textile wearable wireless node, for operation in the 2.45 GHz industrial, scientific and medical (ISM) band, is proposed. It consists of a dual-polarised textile patch antenna with integrated microcontroller, sensor, memory and transceiver with receive diversity. Integrated into a garment, the flexible unit may serve for fall detection, as well as for patient or rescue-worker monitoring. Fragile and lossy interconnections are eliminated. They are replaced by very short radiofrequency signal paths in the antenna feed plane, reducing electromagnetic compatibility and signal integrity problems. The compact and flexible module combines sensing and wireless channel monitoring functionality with reliable and energy-efficient off-body wireless communication capability, by fully exploiting dual polarisation diversity. By integrating a battery, a fully autonomous and flexible system is obtained. This novel textile wireless node was validated, both in flat and bent state, in the anechoic chamber, assessing the characteristics of the integrated system in free-space conditions. Moreover, its performance was verified in various real-world conditions, integrated into a firefighter garment, and used as an autonomous body-centric measurement device

Neutron induced alpha production from carbon between 18 and 22 MeV

Cross sections for neutron induced alpha production in carbon were measured at seventeen energies between 18 and 22 MeV, using a deuterated anthracene crystal as both target and detector. Pulse shape discrimination was employed to separate the alphas and elastically scattered deuterons from the other reaction products. Published (n,d) elastic scattering data were used as a standard to obtain the alpha production cross sections. Comparison with available measurements shows good agreemen

The Impact of Welfare Reform on Leaver Characteristics, Employment and Recidivism: An Analysis of Maryland and Missouri

State and federal reforms of the 1990s transformed the U.S. cash assistance program for single parents and their children. Despite an extensive literature examining these changes and their impacts, there have been few studies that consider the effects of these reforms from the perspective of the recent period. The analysis here focuses on the characteristics and employment of welfare recipients in Maryland and Missouri, 1991-2004. We find that there has been only modest change in the observable characteristics of those entering, remaining on or leaving welfare, but the importance of employment has grown for each of these groups. We also examine the dynamics of employment and welfare recidivism, comparing cohorts of leavers prior to and after welfare reform. We find that after welfare reform leavers are much more likely to be working. Although in Maryland those working have earnings that are somewhat below employed leavers prior to reform, in Missouri earnings for employed leavers are unchanged. In both states, the types of jobs leavers hold have not changed substantially, and leavers are less likely to return to welfare following reform.welfare reform

Impacts of climate change on public health in Australia

Provides information, opinions and recommendations relevant to assessing the potential impacts of climate change on public health in Australia, as well as guidelines for decision-making in responding to these impacts. It aims to: draw attention to the potential impacts of climate change on health in Australia; discuss the policies and issues related to the impacts of climate change on health; present prioritised recommendations to decision-makers on policies and practices which may assist mitigation of and adaptation to the most serious of the identified impacts; provide guidance which will assist appropriate people and agencies to allocate resources to the highest priority problems; and provide a comprehensive list of references which provide reliable evidence about the potential impacts of climate change on health in Australia. The immediate and longer-term impacts of climate change have the potential to affect Australian health and social environments seriously, and as such, demand and deserve attention by Federal and State Governments and agencies within the Australian public health sector. Policymakers are faced with pressing issues of funding and delivering health services for an ageing society with an ever increasing burden of chronic disease and expectations of access to high-technology, high cost interventions. However, the impacts of future climate change on public health may potentially generate very large healthcare costs if current strategies for healthcare are inadequate. Successful advocacy of new policies and practices by credible and influential groups must use language which can be understood by the people who are to be influenced. This advocacy must be supported by reliable evidence. Climate-related catastrophes (droughts, floods, cyclones, other storms, bush fires) occur frequently in Australia. The demonstrably high variability in the incidence and severity of such phenomena present a challenge to scientists to discover and demonstrate any correlations between the catastrophes and the slow changes of climatic indices due to climate change. Public health organisations must start to develop alternative, more effective, practices to manage the complex issues related to climate change while continuing to implement their traditional primary, secondary and tertiary preventive models. A new approach, based on ecological principles, will be required to navigate through the complex and interrelating health causes. The public health sector must strengthen existing approaches for effective climate change adaptation strategies, including assessing regional health risks to identify vulnerable and resilient populations, collecting enhanced surveillance data and developing monitoring indicators. This approach must be based on: providing sound scientific evidence for predicting the likely outcomes and thus to take preventive or responsive action; and reorienting the public health sector towards greater comprehension and use of ecological understandings and approaches. Recommendations Politicians, health bureaucrats and other interested parties must formulate comprehensive, coherent policies to address the direct and indirect impacts of climate change on public health, including allocation of appropriate financial resources as part of a National Plan for Health in Responding to Climate Change. The National Health and Medical Research Council should be tasked with ensuring coordinated, comprehensive funding to support research into the health impacts of climate change. Research organisations and health institutions must collaborate to develop cost-effective, long-term, longitudinal studies on the impacts of climate change on the physical, biological and social environments that will affect Australian’s public health. Advocates must develop proposals which demonstrate cost savings to government over three to six years, or one or two electoral cycles. Little will be achieved in the current fiscal environment if proposed policies and practices will incur significant new budgetary expenses to governments or their agencies. Managing the impacts of climate change on public health will also involve several other sectors, such as water, planning, building, housing and transport infrastructure. Appropriate institutions should work towards a multi-level, interdisciplinary and integrated response to raise the importance of the impacts of climate change on public health. A comprehensive surveillance system would monitor the inter-relationship of environmental, social and health factors. Observational studies are important to monitor recent and present disease patterns and incidence to inform modelling of future disease patterns. They could also provide baselines for environmental health indicators, which can periodically be monitored and measured in order to inform program evaluation. The public health sector must integrate planned, evidence-based adaptations into existing preventive activities. Useful methodologies might include: a risk assessment approach such as Health Impact Assessment (HIA); an appropriate range of Environmental Health Indicators (EHIs); a “Driving force-Pressure-State-Exposure-Effect-Action (DPSEEA) framework”; and a systematic ecological health framework. The opposite of vulnerability is resilience – our capacity to respond to challenging or new circumstances. The factors which encourage resilience needs to be better understood. The public health sector must communicate concepts of risk, and develop strategies to encourage greater resilience. To understand how we can minimise vulnerability of individuals and communities to climate change we must identify those populations which are most at risk, including those for whom climate change will act as a stress multiplier for existing public health problems. The health sector must communicate climate change as a human health issue rather than just an “environmental problem”. The focus should be on effective, realistic and sustainable solutions rather than problems characterised as bleak and unresolvable