Pre-test diets and experimental diets.

<p>Pre-test diet A was fed to the first batch of minipigs (A) and pre-test diet B was fed to the second batch of minipigs (B) during the two weeks of acclimatisation before the test diets were applied. Low fat, high carbohydrate (LFHC); low fat, high carbohydrate/sucrose (LFHS); high fat/cholesterol, low carbohydrate (HFLC); Atwater fuel energy (AFE); Metabolisable energy (ME). Data have previously been published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093821#pone.0093821-Haagensen1" target="_blank">[47]</a>.</p

Body weight (kg) of minipigs by the end of the study.

<p>Low fat, high carbohydrate (LFHC); high fat/cholesterol, low carbohydrate (HFLC); low fat, high carbohydrate/sucrose (LFHS). Analyses of differences in body weight between dietary treatments were carried out by a one-way analysis of variance followed by an unpaired t-test for pairwise comparisons. Differences between diets: *P&lt;0.05. Data have previously been published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093821#pone.0093821-Haagensen1" target="_blank">[47]</a>.</p

Categories and definitions of recorded skin lesions.

<p>Categories and definitions of recorded skin lesions.</p

Blood parameters.

<p>Concentrations (mean ±SD) of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoproteins (LDL), triglycerides (TG), glucose, fructosamine and insulin from blood samples at baseline (B), age 13 weeks, medium (M) and at euthanasia (E). Low fat, high carbohydrate (LFHC); high fat/cholesterol, low carbohydrate (HFLC); low fat, high carbohydrate/sucrose (LFHS). Differences between diets: *P&lt;0.05, ***P&lt;0.001, comparisons regarding only one of the other diets, are marked by the abbreviation of the respective diet. Differences within diets: a = P&lt;0.05, b = P&lt;0.01, c = P&lt;0.001, comparisons are marked by the abbreviation(s) of blood samples. TG data has previously been published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093821#pone.0093821-Haagensen1" target="_blank">[47]</a>.</p

Definitions of behavioural elements and locations in the Novel object test.

<p>Definitions of behavioural elements and locations in the Novel object test.</p

Time budget and frequencies of behaviours for minipigs in their home pen.

<p>Data are presented as % observations (mean±SD). Low fat, high carbohydrate (LFHC); high fat/cholesterol, low carbohydrate (HFLC); low fat, high carbohydrate/sucrose (LFHS); **P&lt;0.01, ***P&lt;0.001. If the comparison regards only one of the other diets, this is marked by the abbreviation of the respective diet.</p

a–b. Skin lesions on head and body of minipigs.

<p>% minipigs (mean of 14 recordings ×24 minipigs and SD) with head lesions in Category 1 and 2; Category 1 (0–1 lesion), Category 2 (2–5 lesions), and body lesions in Category 1, 2 and 3; Category 1 (0–1 lesion), Category 2 (2–5 lesions), Category 3 (6–10 lesions). Low fat, high carbohydrate (LFHC); high fat/cholesterol, low carbohydrate (HFLC); low fat, high carbohydrate/sucrose (LFHS), Comparisons between the three dietary treatments were done by a two-tailed non-parametric Kruskal-Wallis test, followed by a Mann Whitney U test for pairwise comparisons. Differences between diets: *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001.</p

Pilot study results.

<p>Data are presented as % observations (mean±SD) of aggression and % minipigs with &lt;5 lesions and between 6–10 lesions on the body, **P&lt;0.01.</p

Heart rate during Human approach test and Novel object test.

<p>Heart rate in beats per minute presented as mean±SD. Differences in mean heart rates between dietary treatments was analysed using a one way analysis of variance followed by a two-tailed unpaired t-test,</p><p>**P&lt;0.01 for comparison with LFHC diet; low fat, high carbohydrate (LFHC); high fat/cholesterol, low carbohydrate (HFLC); low fat, high carbohydrate/sucrose (LFHS).</p

Ethogram of behavioural elements for the spontaneous behavioural observations.

<p>Ethogram of behavioural elements for the spontaneous behavioural observations.</p