1 research outputs found
Polymerizable Peptide Copolymer Coatings for the Control of Biointerfacial Interactions
The
effective control over biointerfacial interactions is essential
for a broad range of biomedical applications in vitro and in vivo
such as biosensors, cell culture tools and implantable devices. Here,
our aim was to develop a coating strategy that is transferable between
different substrate materials and can effectively suppress nonspecific
protein adsorption and hence reduce cell attachment while also presenting
bioactive signals to enable specific cell–material interactions.
In a first step an allylamine plasma polymer coating was applied,
followed by the covalent immobilization of a macroinitiator carrying
iniferter functionalities in the side chains. Subsequently, copolymers
with different molar ratios of acrylamide and a polymerizable peptide
containing the sequence Arg-Gly-Asp (RGD) were grafted via surface
initiated free radical polymerization. X-ray photoelectron spectroscopy
(XPS) was used to confirm the success of each coating step. The cellular
response to these coatings was evaluated using L929 mouse fibroblast
cell culture assays for up to 24 h. Cell attachment was significantly
reduced on acrylamide homopolymer coatings and negative control surfaces
representing a polymerizable peptide containing the nonbioactive Arg-Ala-Asp
(RAD) sequence. In contrast, cell attachment was increased with increasing
polymerizable RGD peptide ratios in the copolymer. The combination
of acrylamide-terminated peptide sequences in combination with acrylamide
provides a simple and versatile route to surfaces that combine low
nonspecific protein adsorption and the display of controlled densities
of bioactive signals and is expected to be translated into a number
of biomedical applications in vitro and in vivo