Proceedings of the Twenty-Eighth Annual Biochemical Engineering Symposium

This series of Annual Biochemical Engineering Symposia was started in 1971 by Professors Larry E. Erickson of Kansas State University and Peter]. Reilly; then of the University of Nebraska-Lincoln. It is designed for graduate students and occasionally postdoctoral fellows and undergraduates to present the results of their research and the directions of their future work to audiences not so familiar as those at their home institutions but not so seemingly intimidating as those at national professional meetings. It also serves as a vehicle for those engaged in similar lines of research to become acquainted with each other and with each others\u27 work. To that end, discussions both during the meeting and at social events are encouraged. To improve students\u27 skills in the writing of articles, in general those that follow were first drafted by the students who presented the work. In recent years, the symposium has rotated among the University of Colorado, Boulder; Colorado State University; Iowa State University; the University of Kansas; Kansas State University; the University of Missouri, Columbia; and the University of Oklahoma. This 28th Annual Biochemical Engineering Symposium took place at Iowa State University; Ames, lA, on October 3, 1998. Contents - Oral Presentations - High Pressure Crystallization of Purafect Subtilisin - Ruta Y. Waghmare, Jonathan N. Webb, Theodore W Randolph, Maurice A. Larson, and Charles E. Glatz, Department of Chemical Engineering, Iowa State University and Department of Chemical Engineering, University of Colorado. High Pressure Refolding of Protein Aggregates - Richard J. St. John, John E Carpenter, and Theodore W. Randolph, Center of Pharmaceutical Biotechnology and Department of Chemical Engineering, University of Colorado. Purification of 13-Glucuronidase and Its Fusions from Spiked Canola Protein Extract by Anion Exchange Chromatography - Chenming Zhang and Charles E. Glatz, Department of Chemical Engineering, Iowa State University. Biodegradation and Remediation of Methyl tert-Butyl Ether - Qizhi Zhang, Lawrence C. Davis, and Larry E. Erickson. Departments of Chemical Engineering and Biochemistry; Kansas State University. Function and Degradation of Benzotriazole - Doug Lupher, L. C. Davis, and L. E. Erickson. Departments of Chemical Engineering and Biochemistry, Kansas State University. Apoptosis and Bcl-2 Expression in Hybridoma Cell Cultures - Mark C. Mowry, Marit Nilsen-Hamilton, and Carole A. Heath, Departments of Chemical Engineering and Biochemistry; Biophysics, and Molecular Biology, Iowa State University. Mathematical Modeling of Fluid Dynamics in the Eye - Jeffrey Heys and Victor H. Barocas, Department of Chemical Engineering, University of Colorado. The Influence of the Conduit on Regeneration in a Bioartificial Nerve Graft - Gregory E. Rutkowski and Carole A. Heath, Department of Chemical Engineering, Iowa State University. - Posters - Effect of Processing on the Recovery of Recombinant 13-Glucuronidase from Transgenic Canola - Y. Bail and Z. L. Nikolov, Departments of Chemical Engineering and Food Science and Human Nutrition, Iowa State University. A Novel Technique Using Photopolymerization for Cell Encapsulation as a Method for Cartilage Regeneration - S. J. Bryant, C. R. Nuttelman, and K. S. Anseth, Department of Chemical Engineering, University of Colorado. Intermittent Compressive Effects on Cartilage Matrix Formation - Scott E. Carver and Carole A. Heath, Department of Chemical Engineering, Iowa State University. Size Classification of an Iron-Carbon Magnetic Particulate for a Localized Drug Delivery System - Ryan P. Cooper, Melinda Roskos, John E Doyle, Theresa Longin, Kelly Rakes, and Paul Todd, Department of Chemical Engineering, University of Colorado. A Coupled Model of the Cardiorespiratory and Thermoregulatory Effects: Dehydration Effects - Dawn Downey and Richard C. Seagrave, Department of Chemical Engineering and Biomedical Engineering Program, Iowa State University. Uptake of Tricholorethylene by Plants - Jiang Hu, L. C. Davis, and L. E. Erickson, Departments of Chemical Engineering and Biochemistry, Kansas State University. Controlled Fenamiphos Release Using Starch Based Polymer Blend Encapsulation - S. Iwashita, N. Pednekar, and M. Z. Southard, Department of Chemical and Petroleum Engineering, University of Kansas. Phytoremediation of Washwater Sediments Contaminated with Petroleum Hydrocarbons - P. Kulakow, R. Karthikeyan, B. Leven, K. R. Mankin, and L. E. Erickson\ Departments of Agronomy, Biological and Agricultural Engineering, Great Plains/Rocky Mountain Hazardous Substance Research Center, and Chemical Engineering, Kansas State University. Automated Docking of Maltose, 2-Deoxymaltose, and Maltotetraose into the Soybean β-Amylase Active Site - Alain Laederachl, Michael K. Dowd, Pedro M. Coutinho, and Peter J. Reilly, Department of Chemical Engineering, Iowa State University, Southern Regional Research Center, USDA, New Orleans, LA, and Centre de Recherches sur les Macromolecules Vegetales, CNRS, F-38041 Grenoble cedex, France. Customized Injectable Pluronic® Gels for Sustained Drug Delivery - Theodore Moore\ Michael Oberhaus, Nita Pandit, and Surya Mallapragada, Department of Chemical Engineering, Iowa State University, and College of Pharmacy and Health Sciences, Drake University. Evaluation of Surgical Limb Lengthening Through Radiographic Absorptiometry - Sridhar Sunderam, Brad W Olney, and Marylee Z. Southard, Departments of Chemical and Petroleum Engineering and Orthopedic Surgery, University of Kansas. Controlled Release and Modeling of Drug Distribution in Vitreous - Jing Xu, Victor H. Barocas, and Theodore W Randolph, Department of Chemical Engineering, University of Colorado.https://lib.dr.iastate.edu/bce_proceedings/1033/thumbnail.jp

Proceedings of the Thirty-third Annual Biochemical Engineering Symposium

This series of Annual Biochemical Engineering Symposia was started in 1971 by Professor Larry E. Erickson of Kansas State University and Peter J. Reilly, then of the University of Nebraska-Lincoln. It is designed for graduate students and occasionally undergraduates and postdoctoral fellows to present the results of their research and directions of their future work to audiences not so familiar as those at their home institutions but not so seemingly intimidating as those at national professional meetings. It also serves as a vehicle for those engaged in similar lines of research to become acquainted with each other and with each other\u27s work. To that end, discussions both during the meeting. and at social events attached to it are encouraged. To improve students\u27 skills in writing articles, in general those that follow were first drafted by the students who presented the work reported in them. The 33 symposia have rotated among the University of Colorado, Boulder; Colorado State University; Iowa State University; Kansas State University; the University of Missouri, Columbia; the University of Nebraska-Lincoln; and the University of Oklahoma. This 33rd Annual Biochemical Engineering Symposium took place on April 24, 2004 at the University of Nebraska-Lincoln with Professor Michael M. Meagher as host. It should be noted that the 32 d Symposium was held on October 5, 2002. There was no symposium in 2003. Therefore symposium numbering is now in line with the starting year (1971 + 33 symposia = 2004 ). Fourteen papers were delivered orally at the symposium and there were a number of posters. These Proceedings present articles from nearly all the oral presentations and from several posters. Contents Novel Pentablock Copolymers as Non-Viral Vectors for Gene Therapy against Cancer - Ankit Agarwal, Robert Unfer, and Surya Mallapragada, Department of Chemical Engineering, Iowa State University, Ames, Iowa, USA 50011, Iowa Cancer Research Foundation, Urbandale, lA 50322 Lipase-Catalyzed Esterification of Geraniol in Ionic Liquid [bmim]PF6 - Donifan Barahona, Peter H. Pfromm, and Mary E. Rezac, Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506 Conformational Analysis of Gossypol and Its Derivatives Using MM3 - Chase L. Beisel, Michael K. Dowd, and Peter J. Reilly, Department of Chemical Engineering, Iowa State University, Ames, lA 50011, Southern Regional Research Center, ARS, USDA, New Orleans, LA 70179 Production of Bacterial Cellulose by Acetobacter xylinum in Static Culture and Its Properties - Sasivimon Chittrakorn, Charles E. Walker, and Larry E. Erickson, Departments of Grain Science and Industry and Chemical Engineering, Kansas State University, Manhattan, KS 66506 A Better Global Resolution Function and a Novel Iterative Stochastic Search Method for Optimization of HPLC Separation - Yandi Dharmadi, and Ramon Gonzalez, Departments of Chemical Engineering and Food Science and Human Nutrition, Iowa State University, Ames, lA 50011 Synchrotron Fourier Transform Infrared Microspectroscopy as a Tool to Monitor the Fate of Organic Contaminants in Plants - Kenneth M. Dokken, Lawrence C. Davis, Larry E. Erickson, and Nebojsa Marinkovic, Departments of Biochemistry and Chemical Engineering, Kansas State University, Manhattan, KS 66506; Albert Einstein Center for Synchrotron Biosciences, Beamline U2B, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY 11973 Ranque-Hilsch Vortex Tube Thermocycler for Fast DNA Amplification and Real-Time Optical Detection - Ryan J. Ebmeier, Scott E. Whitnel, Amitabha Sarkar, Michael Nelson, Nisha V. Padhye, George Gogos, and Hendrik J. Viljoen, Departments of Mechanical and Chemical Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, Megabase Research Products, 4711 Huntington Ave., Suite 2W, Lincoln, NE 68504 H-NMR Study of the Structure of C. antarctica Lipase Bin Hexane-Water Mixtures - Yvonne Hoffmann, Yu-Xi Gong, Om Prakash, Peter H. Pfromm, Mary E. Rezac, and Peter Czermak, Departments of Chemical Engineering and Biochemistry, Kansas State University, Manhattan, KS 66506; University of Applied Sciences Giessen, Giessen, Germany Remediation of Sites Contaminated by Oil Refinery Operations, S. Khaitan, S. Kalainesan, 75 L. E. Erickson, P. Kulakow, S. Martin, R. Karthikeyan, S. L. L. Hutchinson, and L. C. Davis, Department of Chemical Engineering, Department of Agronomy, Center for Hazardous Substance Research, Department of Biological and Agricultural Engineering, and Department of Biochemistry, Kansas State University, Manhattan, KS 66506 Biodegradation of Tertiary Butyl Mercaptan in Soil under Aerobic Conditions - S. Kalainesan, 85 L. E. Erickson, S. L. L. Hutchinson and R. Karthikeyan, Departments of Chemical Engineering and Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506 Probing the Substrate Specificity of Streptomyces Phospholipase D by Automated Docking, Patrick D. McMullen, Christopher L. Aikens, and Peter J. Reilly, Department of Chemical Engineering, Iowa State University, Ames, lA 50011 On-Line Predictive Model for Cell Mass in Pichia pastorisFermentation Using Neural Networks, Sreenivasula Ramireddy and Michael M. Meagher, Department of Chemical Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588 Toxicity of RDX on Germination of Arabidopsis thaliana - Sarah Rollo, Hangsik Moon, Murali Subramanian, David J. Oliver, and Jacqueline V. Shanks, Departments of Chemical Engineering and Genetics, Development and Cell Biologly, Iowa State University, Ames, lA 50011 Biodegradation of Tetrachloroethylene (PCE) in Soil and Groundwater - S. Santharam, J. lbbini, L. C. Davis, and L. E. Erickson, Departments of Chemical Engineering and Biochemistry, Kansas State University, Manhattan, KS 66506 Sorption of the Mal odorant, Tertiary Butyl Mercaptan, to Interior Surfaces - Aruna Suravajjala, Stacy L. Hutchinson, Larry E. Erickson, and Alok Bhandari, Departments of Civil Engineering, Biological and Agricultural Engineering, and Chemical Engineering, Kansas State University, Manhattan, KS 66506https://lib.dr.iastate.edu/bce_proceedings/1028/thumbnail.jp

Proceedings of the Thirty-second Annual Biochemical Engineering Symposium

This series of Annual Biochemical Engineering Symposia was started in 1971 by Professors Larry E. Erickson of Kansas State University and Peter J. Reilly, then of the University of Nebraska-Lincoln. It is designed for graduate students and occasionally postdoctoral fellows and undergraduates to present the results of their research and directions of their future work to audiences not so familiar as those at their home institutions but not so seemingly intimidating as those at national professional meetings. It also serves as a vehicle for those engaged in similar lines of research to become acquainted with each other and with each others\u27 work. To that end, discussions both during the meeting and at social events are encouraged. To improve students\u27 skills in writing articles, in general those that follow were first drafted by the students who presented the work reported in them. The 32 symposia have rotated among the University of Colorado, Boulder; Colorado State University; Iowa State University; Kansas State University; the University of Missouri, Columbia; the University of Nebraska-Lincoln; and the University of Oklahoma. This 32nd Annual Biochemical Engineering Symposium took place at Iowa State University, Ames, Iowa, on October 5, 2003. Contents Mechanical performance ofbiomimetic hydrogels - Sharon Hagan and Stevin H. Gehrke, Kansas State University Encapsulation, stabilization, and release of proteins from biodegradable polyanhydrides - Amy S. Determan, Matt Kipper, Hajime Takano, Marc Porter, and Balaji Narasimhan, Iowa State University Predicting the performance of adsorbents based on EDTP A-modified zirconia particles - Sabyasachi Sarkar and Anuradha Subramanian, University of Nebraska-Lincoln Kinetic analysis of CaC03 precipitation by Siran™-immobilized Bacillus pasteurii - Jacklyne J. Lippert and Sookie S. Bang, South Dakota School of Mines and Technology Automated docking of phospholipids to the phospholipase D active site: Insight into the catalytic mechanism - Christopher L. Aikens, Alain Laederach, and Peter J. Reilly, Iowa State University Development of Bacillus pasteurii urease-negative mutants - Johnna K. Roth and Sookie S. Bang, South Dakota School of Mines and Technology Recovery of enzyme byproducts during recombinant protein recovery from transgenic plants - Yandi Dharmadi, Qinlong Chang, and Charles E. Glatz, Iowa State University Determination of the biomass composition of hairy roots of Catharanthus roseus - Omar Gonzalez Rivera, Ganesh Sriram, and Jacqueline V. Shanks, Iowa State University Modification of environmental variables to understand transformation of xenobiotics on root surface: Impact of altering variables on phytotransformation processes - Sigifredo Castro, Lawrence C. Davis, and Larry E. Erickson, Kansas State University Development of NMR tools to obtain metabolic flux maps for soybean seeds - Vidya Iyer, Ganesh Sriram, D. Bruce Fulton, Mark E. Westgate, Martin H. Spalding, and Jacqueline V. Shanks, Iowa State Universityhttps://lib.dr.iastate.edu/bce_proceedings/1027/thumbnail.jp

Proceedings of the 37th Annual Biochemical Engineering Symposium

This Proceedings contains papers from most of the works presented at the 37th Annual Biochemical Engineering Symposium, held at Iowa State University on April 26, 2008. This Symposium first occurred in 1971, and has continued annually with a one-year break since then, being hosted in various years by the University of Colorado, Boulder; Colorado State University; Iowa State University; Kansas State University; the University of Missouri, Columbia; the University of Nebraska-Lincoln; the University of Oklahoma; and the South Dakota School of Mines and Technology. It is meant for graduate students and occasional undergraduates to become familiar with making oral presentations and presenting posters in front of audiences not completely familiar to them, but on the other hand not so foreign as those at a national meeting. Contents Tangential flow filtration for virus purification - David L. Grzenia, Jonathan O. Carlson, and S. Ranil Wickramasinghe, Departments of Chemical and Biological Engineering and Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523 FT -IR studies on stability of proteins adsorbed to polysaccharide-based polyelectrolyte multilayers - Jorge Almodóvar, Laura Dempsey, and Matt J. Kipper, Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15260; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523 Intracellular trafficking of pentablock copolymer/DNA polyplexes in cultured human cancer and normal cells for gene delivery - Bingqi Zhang and Surya Mallapragada Department of Chemical and Biological Engineering, Iowa State University, Ames, lA 50011 Effect of agitation, added solids, and reductant concentration on biological methane production in coal slurries - Khang D. Vo and Patrick Gilcrease Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701 Metabolic engineering of Catharanthus roseus hairy roots for terpenoid indole alkaloid production under light and dark conditions - Guy W. Sander, Susan I. Gibson, Ka-Yiu San, and Jacqueline V. Shanks, Department of Chemical and Biological Engineering, Iowa State University, Ames, lA 50011; Department of Plant Biology, University of Minnesota, St. Paul, MN 55108; Department of Bioengineering, Rice University, Houston, TX 77251 Amphiphilic polyanhydride chemistry affects monocytic association of nanospheres - Bret D. Ulery, Kevin Pustulka/ Yashdeep Phanse, Bryan Bellaire, and Balaji Narasimhan Departments of Chemical and Biological Engineering and Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames, Iowa, 50011 Properties of a glycoside hydrolase family 44 endoglucanase from Clostridium acetobutylicum - Christopher D. Warner, Taran C. Shilling, Michael J. Linnen, Clark F. Ford, and Peter J. Reilly, Departments of Chemical and Biological Engineering and Food Science and Human Nutrition, Iowa State University, Ames, lA 50011https://lib.dr.iastate.edu/bce_proceedings/1036/thumbnail.jp

A Simple and Flexible Dynamic Approach to Foreign Direct Investment Growth: The Canada-United States Relationship in the Context of Free Trade.

This paper asks a simple question: Did Wilfred Laurier’s dream of free trade with the United States, when it came to fruition in 1989, also impact on foreign direct investment (FDI) into Canada by US multinationals? This paper argues that the customary static econometric approach found in the FDI literature, along with the assumption that policy changes influence only the intercept term, are inadequate to address the question. Instead we introduce an innovative dynamic framework to support the testing of hypotheses on behavioural changes in the variables using a structural break framework. A key conclusion is that prior to signing the free trade agreement US FDI responded only to current growth in the Canadian economy, in a unitary fashion, and current exchange rate shifts. This can be described as a static relationship. The implementation of the free trade agreements between Canada and the USA increased the responsiveness of US FDI to growth in the Canadian economy by a factor greater than two. Furthermore, dynamics are found in the form of a lagged effect for changes in the growth in the Canadian economy and interest rate differentials. These conclusions challenge the dominant view, including that in official policy circles, that the free trade agreement had no impact on US firms’ FDI decisions in Canada. Note: Previous versions of this paper were entitled: “A Simple and Flexible Dynamic Approach to Foreign Direct Investment Growth: Did Canada Benefit From the Free Trade Agreements with the United States?”Canada-United States, foreign direct investment, empirical relationship

Clinical Manifestations of Hereditary Hemorrhagic Telangiectasia

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72156/1/j.1572-0241.1984.tb05137.x.pd

Ferromagnetic Luttinger Liquids

We study weak itinerant ferromagnetism in one-dimensional Fermi systems using perturbation theory and bosonization. We find that longitudinal spin fluctuations propagate ballistically with velocity v_m << v_F, where v_F is the Fermi velocity. This leads to a large anomalous dimension in the spin-channel and strong algebraic singularities in the single-particle spectral function and in the transverse structure factor for momentum transfers q ~ 2 Delta/v_F, where 2 Delta is the exchange splitting.Comment: 4 pages, 3 figure

Towards standard setting for patient-reported outcomes in the NHS homeopathic hospitals

We report findings from a pilot data collection study within a programme of quality assurance, improvement and development across all five homeopathic hospitals in the UK National Health Service (NHS).&lt;p&gt;&lt;/p&gt; &lt;b&gt;Aims&lt;/b&gt; (1) To pilot the collection of clinical data in the homeopathic hospital outpatient setting, recording patient-reported outcome since first appointment; (2) to sample the range of medical complaints that secondary-care doctors treat using homeopathy, and thus identify the nature and complexity of complaints most frequently treated nationally; (3) to present a cross section of outcome scores by appointment number, including that for the most frequently treated medical complaints; (4) to explore approaches to standard setting for homeopathic practice outcome in patients treated at the homeopathic hospitals.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Methods&lt;/b&gt; A total of 51 medical practitioners took part in data collection over a 4-week period. Consecutive patient appointments were recorded under the headings: (1) date of first appointment in the current series; (2) appointment number; (3) age of patient; (4) sex of patient; (5) main medical complaint being treated; (6) whether other main medical complaint(s); (7) patient-reported change in health, using Outcome Related to Impact on Daily Living (ORIDL) and its derivative, the ORIDL Profile Score (ORIDL-PS; range, –4 to +4, where a score &#8804;−2 or &#8805;+2 indicates an effect on the quality of a patient's daily life); (8) receipt of other complementary medicine for their main medical complaint.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Results&lt;/b&gt; The distribution of patient age was bimodal: main peak, 49 years; secondary peak, 6 years. Male:female ratio was 1:3.5. Data were recorded on a total of 1797 individual patients: 195 first appointments, 1602 follow-ups (FUs). Size of clinical service and proportion of patients who attended more than six visits varied between hospitals. A total of 235 different medical complaints were reported. The 30 most commonly treated complaints were (in decreasing order of frequency): eczema; chronic fatigue syndrome (CFS); menopausal disorder; osteoarthritis; depression; breast cancer; rheumatoid arthritis; asthma; anxiety; irritable bowel syndrome; multiple sclerosis; psoriasis; allergy (unspecified); fibromyalgia; migraine; premenstrual syndrome; chronic rhinitis; headache; vitiligo; seasonal allergic rhinitis; chronic intractable pain; insomnia; ulcerative colitis; acne; psoriatic arthropathy; urticaria; ovarian cancer; attention-deficit hyperactivity disorder (ADHD); epilepsy; sinusitis. The proportion of patients with important co-morbidity was higher in those seen after visit 6 (56.9%) compared with those seen up to and including that point (40.7%; P &lt; 0.001). The proportion of FU patients reporting ORIDL-PS &#8805; +2 (improvement affecting daily living) increased overall with appointment number: 34.5% of patients at visit 2 and 59.3% of patients at visit 6, for example. Amongst the four most frequently treated complaints, the proportion of patients that reported ORIDL-PS &#8805; +2 at visit numbers greater than 6 varied between 59.3% (CFS) and 73.3% (menopausal disorder).&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusions&lt;/b&gt; We have successfully piloted a process of national clinical data collection using patient-reported outcome in homeopathic hospital outpatients, identifying a wide range and complexity of medical complaints treated in that setting. After a series of homeopathy appointments, a high proportion of patients, often representing “effectiveness gaps” for conventional medical treatment, reported improvement in health affecting their daily living. These pilot findings are informing our developing programme of standard setting for homeopathic care in the hospital outpatient context