Diagrammatic representation of the distribution of unique probe-sets of the serotype 1/2a, PFGE pattern combination IV 2011 and absent in the PFGE pattern combination I and III strains based on their functions.

<p>The numbers of probe-sets were indicated in the periphery of the pie-chart.</p

Relatedness analysis of the compatible parsimony informative genes from the 15 cantaloupe associated outbreak isolates, a shrimp isolate (LS693) and 15 <i>L. monocytogenes</i> isolates from several unrelated listeriosis episodes/outbreaks.

<p>The tree was generated from the concatenated gene contents using neighbor joining with the uncorrected p distance. The numbers in red color indicate the cantaloupe associated outbreak and the shrimp isolates. The boxes indicate the close-up portions of the cantaloupe outbreak strains (green; serotype 1/2b and blue; serotype 1/2a). Scale bar represents number of gene differences (present or absent) per gene site. The Roman numerals represent the PFGE pattern combination.</p

<i>Listeria monocytogenes</i> strains used in this study.

a<p>PFGE pattern combinations were assigned based on the <i>AscI</i> pattern and the <i>ApaI</i> pattern as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042448#pone-0042448-g001" target="_blank">Figure 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042448#pone-0042448-t002" target="_blank">Table 2</a>.</p>b<p>Non-outbreak related.</p

Comparison of the RMA summarized probe-set intensities.

<p>(A) pattern combination I isolates - LS742 and LS743; (B) pattern combination I (LS742) and pattern combination III (LS746); (C) pattern combination I (LS742) and pattern combination IV (LS747); (D) pattern combination III (LS746) and pattern combination IV(LS747). Red dots indicate RMA summarized probe-set intensity differences of less than or equal to 2-fold between two strains. Blue dots indicate RMA summarized probe-set intensity differences of more than 2-fold between the two strains.</p

PFGE profiles <i>of Listeria monocytogenes</i> cantaloupe outbreak strains.

<p><i>AscI</i> patterns are shown in panel A: lane 2: GX6A16.0099 (III); lane 3: GX6A16.0019 (II); lane 4: GX6A16.0001 (IV); lane 5: GX6A16.0029 (I) and <i>ApaI</i> patterns in panel B lane 2: GX6A12.0001 (III); lane 3: GX6A12.0227 (II); lane 4: GX6A12.0001 (IV); lane 5: GX6A12.0069 (I). Lane 1 in both panels is <i>Salmonella braenderup</i> H9812 standard (digested by <i>XbaI</i>). The Roman numerals represent the PFGE pattern combination.</p

Hierarchical clustering based on the Robust Multi Array (RMA) analysis.

<p>Strains highlighted in red indicated the serotype 1/2a strains from the 2011 Cantaloupe outbreak.</p

Relatedness analysis of the compatible parsimony informative genes from the 9 cantaloupe associated outbreak isolates, a shrimp isolate (LS693) and 7 unrelated <i>L. monocytogenes</i> isolates belonging to serotype 1/2a [<b>28</b>].

<p>The tree was generated from the concatenated gene contents using neighbor joining with the uncorrected p distance. The numbers highlighted in red indicate the cantaloupe associated outbreak and the shrimp isolates. Scale bar represents number of gene differences (present or absent) per gene site. The Roman numerals represent the PFGE pattern combination.</p

Distribution of <i>Listeria monocytogenes</i> isolates producing PFGE pattern combinations.

<p>I- IV from human (grey) or nonhuman (green) sources. Only isolates from August 25–November 15, 2011 (duration of the outbreak) are included.</p

Diagrammatic representation of the distribution of unique probe-sets of the serotype 1/2a 2011 cantaloupe outbreak strains from different PFGE pattern combination groups based on their functions.

<p>A) Probe sets uniquely present in the strains from the PFGE pattern combination I and absent in both PFGE pattern combination III and IV. B) Probe sets uniquely present in the strains from the PFGE pattern combination III and IV and absent in the PFGE pattern combination I. The numbers of probe-sets were indicated in the periphery of the pie-chart.</p

Hierarchical clustering based on the Robust Multi Array (RMA) analysis.

<p>Strains highlighted in red indicated the serotype 1/2b strains from the 2011 Cantaloupe outbreak.</p