Detection of genomic DNAs corresponding to individual (A–G) and complex pathogen samples (H–I) on a universal microarray

<p><b>Copyright information:</b></p><p>Taken from "Diagnostic application of padlock probes—multiplex detection of plant pathogens using universal microarrays"</p><p>Nucleic Acids Research 2005;33(8):e70-e70.</p><p>Published online 28 Apr 2005</p><p>PMCID:PMC1087788.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> The analysed targets were as follows: () , 1 ng; () , 1 ng; () , 1 ng; () AG 4-2, 1 ng; () , 1 ng; () , 1 ng; () , 1 ng; () , 500 pg; , 500 pg and , 500 pg; () , 500 pg; AG 4-2, 500 pg and , 500 pg; () , 500 pg; AG 4-1, 500 pg and , 500 pg; () , 0.5 pg and , 500 pg; and () , 500 pg and , 5 pg

Calibration curves to assess the reproducibility of the Biotrove OpenArray™ platform and the PRI-lock system

<p><b>Copyright information:</b></p><p>Taken from "Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™"</p><p>http://www.biomedcentral.com/1471-2164/8/276</p><p>BMC Genomics 2007;8():276-276.</p><p>Published online 14 Aug 2007</p><p>PMCID:PMC2064939.</p><p></p> () Inter-array variation: The PRI-lock probe mixture was ligated on a 10-fold serial dilution of target and amplified on the Biotrove OpenArray™ platform. Samples were tested on three different OpenArrays™ (n = 3). Data represent averages of six PCR replicates (n = 6). The error bars represent the standard deviations. () Assay-to-assay reproducibility: Three separate whole assay repeats ligation reactions were performed on 10-fold serial dilution of target and amplified in the Biotrove OpenArray™ platform. Data represent averages of 3 separate ligation experiments (n = 3) with each, four PCR replicates (n = 4). Cvalues were normalized using the ILC PRI-lock probe. The error bars represent the standard deviations (Sometimes the standard deviation error bars fall within the data squares)