10 research outputs found
Recommended from our members
Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes
Mast cells (MCs) are heterogeneous cells whose phenotype is modulated by signals received from the local microenvironment. Recent studies have identified the mesenchymal-derived cytokine IL-33 as a potent direct activator of MCs, as well as regulator of their effector phenotype, and have implicated this activity in the ability of mast cells to contribute to murine experimental arthritis. We explored the hypothesis that IL-33 enables participation of synovial MCs in murine K/BxN arthritis by promoting their activation by IgG immune complexes. Compared to wild-type (WT) control mice, transgenic animals lacking the IL-33 receptor ST2 exhibited impaired MC-dependent immune complex-induced vascular permeability (flare) and attenuated K/BxN arthritis. Whereas participation of MCs in this model is mediated by the activating IgG receptor FcγRIII, we pre-incubated bone marrow-derived MCs with IL-33 and found not only direct induction of cytokine release but also a marked increase in FcγRIII-driven production of critical arthritogenic mediators including IL-1β and CXCL2. This “priming” effect was associated with mRNA accumulation rather than altered expression of Fcγ receptors, could be mimicked by co-culture of WT but not ST2−/− MCs with synovial fibroblasts, and was blocked by antibodies against IL-33. In turn, WT but not ST2−/− MCs augmented fibroblast expression of IL-33, forming a positive feedback circuit. Together, these findings confirm a novel role for IL-33 as an amplifier of IgG immune complex-mediated inflammation and identify a potential MC-fibroblast amplification loop dependent on IL-33 and ST2
Mycobacterial infection induces higher interleukin-1β and dysregulated lung inflammation in mice with defective leukocyte NADPH oxidase
<div><p>Granulomatous inflammation causes severe tissue damage in mycobacterial infection while redox status was reported to be crucial in the granulomatous inflammation. Here, we used a NADPH oxidase 2 (NOX2)-deficient mice (<i>Ncf1</i><sup><i>-/-</i></sup>) to investigate the role of leukocyte-produced reactive oxygen species (ROS) in mycobacterium-induced granulomatous inflammation. We found poorly controlled mycobacterial proliferation, significant body weight loss, and a high mortality rate after <i>M</i>. <i>marinum</i> infection in <i>Ncf1</i><sup><i>-/-</i></sup> mice. Moreover, we noticed loose and neutrophilic granulomas and higher levels of interleukin (IL)-1β and neutrophil chemokines in <i>Ncf1</i><sup><i>-/-</i></sup> mice when compared with those in wild type mice. The lack of ROS led to reduced production of IL-1β in macrophages, whereas neutrophil elastase (NE), an abundant product of neutrophils, may potentially exert increased inflammasome-independent protease activity and lead to higher IL-1β production. Moreover, we showed that the abundant NE and IL-1β were present in the caseous granulomatous inflammation of human TB infection. Importantly, blocking of IL-1β with either a specific antibody or a recombinant IL-1 receptor ameliorated the pulmonary inflammation. These findings revealed a novel role of ROS in the early pathogenesis of neutrophilic granulomatous inflammation and suggested a potential role of IL-1 blocking in the treatment of mycobacterial infection in the lung.</p></div
High levels of IL-1β and neutrophilic chemokines in <i>M</i>. <i>marinum</i>-infected <i>Ncf1</i><sup>-/-</sup> mice.
<p>Cytokine and chemokine responses to <i>M</i>. <i>marinum</i> infection (3 x 10<sup>6</sup> CFU). Cytokines of innate immunity including IL-1β (A), TNF-α (B) and IL-6 (C); cytokines of adaptive immunity including IFN-γ (D), IL-10 (E) and IL-17 A(F), and neutrophilc chemokines including CXCL5 (G), CCL5 (H) and CXCL1 (I) were assessed in lung homogenates obtained 7 days and 14 days after <i>M</i>. <i>marinum</i> infection (<i>3</i> x 10<sup>6</sup> CFU). Data represent mean ± sd (n = 4–7 mice each group) The experiments were analyzed with Kruskal-Wallis test. *p < 0.05; **p < 0.005 and repeated with similar results.</p
IL-1β depletion and IL-1 receptor antagonist treatment alleviated the neutrophilic inflammation.
<p>The characteristic pulmonary histopathological images (A) of <i>M</i>. <i>marinum</i>-infected <i>Ncf1</i><sup><i>-/-</i></sup> mice and WT mice on day-5 with and without IL-1β depletion by a monoclonal antibody directed against IL-1β (150μg on day-1). The immunofluorescent stain of neutrophil elastase (NE) (B) also showed an apparently decrease of NE-positive cells after the depletion of IL-1β. The representative histological images (C) of WT and <i>Ncf1</i><sup>-/-</sup>mice treated with and without IL-1 receptor antagonist (Anakinra, 100mg/kg/day for 5days). Percentages of infiltration area (D) were quantified by using Image J. The experiments were analyzed with Kruskal-Wallis test and repeated with similar results. *p < 0.05; **p < 0.005.</p
Expression of neutrophil elastase and IL-1β in the caseous granulomatous inflammation in the human lung tissues of pulmonary tuberculosis.
<p>Formalinized lung tissue (A) of one patient with TB infection. The solid part (B) of the lung tissue showed characteristic caseous granulomatous inflammation with Langhans giant cells, and numerous neutrophils were found within the caseous part (C) of the lung tissues in high power field tissue. Immunohistochemical stain of neutrophil elastase (D) and IL-1β (E) showed abundant neutrophil elastase-positive cells and IL-1β in the granulomatous inflammation.</p
A Higher level of pulmonary inflammation in <i>Ncf1</i><sup><i>-/-</i></sup> mice after <i>M</i>. <i>marinum</i> infection in comparison with the inflammation in WT mice.
<p>Representative gross pictures (A) and cross-sectional histological examinations (B) of <i>M</i>. <i>marinum</i>-infected lungs from WT and <i>Ncf1</i><sup><i>-/-</i></sup> mice at day 7 and day 14 after infection. Acid-fast stains (AFS) (B) at day14 showed abundant AFS-positive bacilli in <i>Ncf1</i><sup>-/-</sup> mice, whereas sparse AFS-positive bacilli were found in WT mice. These experiments were repeated with similar results.</p
The loose and neutrophilic granulomas in <i>Ncf1</i><sup>-/-</sup> mice in comparison with the compact granulomas in WT mice.
<p>The representative immunohistochemical stain of macrophages (F4/80) (A) showed compact aggregation of macrophages in WT mice, whereas macrophages were scattered within the granuloma of <i>Ncf1</i><sup>-/-</sup> mice. The immunofluorescent stain (B) (F4/80: red; Neutrophil elastase (NE): green) illustrated much more NE-positive neutrophils interspersed among macrophages in <i>Ncf1</i><sup>-/-</sup> mice compared with sparse neutrophils in WT mice. (C) Neutrophil and macrophage counts of <i>M</i>. <i>marinum</i>-infected WT and <i>Ncf1</i><sup>-/-</sup>mice at day 7 and day 14 were analyzed by flow cytometry, while CD11b<sup>+</sup>Ly6G<sup>+</sup> represented neutrophils and CD11b<sup>+</sup>F4/80<sup>+</sup> represented macrophages. Data represent mean ± sd of 4 mice from two independent experiments. The experiments were analyzed with Kruskal-Wallis test. *p < 0.05; **p < 0.005. These experiments were repeated twice with similar results.</p
Increased severity and mortality of <i>M</i>. <i>marinum</i> pulmonary infection in <i>Ncf1</i><sup><i>-/-</i></sup> mice.
<p><i>Ncf1</i><sup>-/-</sup> (loss of function mutation in p47phox) and WT controls were intra-tracheal injected with <i>M</i>. <i>marinum</i> (3 x 10<sup>7</sup> CFU). Survival (A) and changes in body weight (B) were monitored over the 28 days period following <i>M</i>. <i>marinum</i> infection in WT (n = 20) and <i>Ncf1</i><sup>-/-</sup> mice (n = 23). The number of viable mycobacteria (C) was determined at 7 days and 14 days after <i>M</i>. <i>marinum</i> infection. Data are shown as a mean log of CFU per paired-lung (5 mice per group). The high correlation between changes in body weight and a number of viable mycobacteria in lungs was demonstrated in (D). Data represented mean ± sd. The experiments were analyzed with Log-rank test (A), Kruskal-Wallis test (C), and Spearman’s rank correlation analysis (D) *p < 0.05; **p < 0.005. These experiments were repeated twice with similar results.</p