MIC distribution of 150 <i>B</i>. <i>bronchiseptica</i> isolates from swine and companion animals after 20 and 24 hours of incubation.

<p>^aData represent the concentrations of amoxicillin; *Asterisked numbers indicate the number of isolates exhibiting MIC values equal to or higher /lower than concentrations of the test range. The white areas represent the tested range of an antimicrobial agent and bars indicate the CLSI recommended breakpoints for resistance of an antimicrobial agent.</p

MIC distribution of 150 <i>B</i>. <i>bronchiseptica</i> isolates from swine and companion animals after 20 and 24 hours of incubation.

<p>^bData represent the concentrations of trimethoprim; *Asterisked numbers indicate the number of isolates exhibiting MIC values equal to or higher /lower than concentrations of the test range. The white areas represent the tested range of an antimicrobial agent.</p

Investigated isolates and detected resistance genes.

<p>Investigated isolates and detected resistance genes.</p

Sample origin.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085656#pone-0085656-g001" target="_blank">Figure 1</a> shows the Germany-wide origin of the 5,229 wound swabs from dogs, cats and horses. Areas are shaped in color with regard to the sample frequency. Black dots represent the sample origin with regard to the postal code. The dot size displays the submission frequency of each veterinary practice/clinic.</p

<i>Spa</i> type frequency and predicted clonal complexes based on <i>spa</i> typing and MLST for representative MRSA from wound infections of canine, feline and equine origin in Germany.

<p><i>Spa</i> type frequency and predicted clonal complexes based on <i>spa</i> typing and MLST for representative MRSA from wound infections of canine, feline and equine origin in Germany.</p

<i>S. aureus</i> proportion in wound samples from companion animals.

<p><i>S. aureus</i> proportion in wound samples from companion animals.</p

Phenotypic resistance for 241 MRSA grouped according to their clonal complexes.

<p>Number (n) and percentage (%) of resistant strains according to VITEK®2 system (bioMérieux, Germany).</p><p>Abbreviations: GEN: gentamicin, KAN: kanamycin, ENR: enrofloxacin, MAR: marbofloxacin, ERY: erythromycin, CLI: clindamycin, TET: tetracycline, NIT: nitrofurantoin, CHL: chloramphenicol and SXT: trimethoprim- sulfamethoxazole.</p

Overview of lineage-diversity among MRSA from dogs, cats and horses.

<p>Overview of lineage-diversity among MRSA from dogs, cats and horses.</p

Descriptive characteristics of dog owners.

<p>Descriptive characteristics of dog owners.</p

Comparative PFGE analysis and strain characteristics of <i>S. pseudintermedius</i> from human and canine origin.

<p>Dendrogram (percent similarity) showing DNA restriction pattern after digestion with <i><u>Sma</u></i>I for all 21 MSSP and MRSP isolates. PFGE analysis by use of bionumerics® (unweighted-pair group method using average linkages), dice coefficient, 1.2% tolerance and 0.5% optimization. Pair 36 comprised the isolates IMT18797 (canine) and IMT18798 (human), which show indistinguishable PFGE pattern. The canine isolate IMT18789 and IMT18828 (human) share an indistinguishable pulsotype as well, although they did not compose a dog/dog owner pair. ¹Strain collection number: IMT: Institute of Microbiology and Epizootics. RKI: Robert Koch-Institute (Wernigerode branch). ²Individual number for each of the participating dog/dog owner pairs. ³Determined by use of VITEK® 2 (bioMerieux) according to the manufacturers instructions. ⁴Allelic profile of the genes <i>pta</i>, <i>cpn</i>60, <i>tuf</i>, 16S rRNA and <i>agr</i>D. Abbreviations: PEN, Penicillin G; GEN, Gentamicin; KAN, Kanamycin; ENR, Enrofloxacin; MAR, Marbofloxacin; ERY, Erythromycin; CLI, Clindamycin; TET, Tetracycline; CHL, Chloramphenicol; i: susceptibility testing result: intermediate. Luk-I: Leukotoxin I (l<i>uk</i>S and <i>luk</i>F), <i>exp</i>A: Exfoliative toxin A (primer: this study), <i>exp</i>B: Exfoliative toxin B.</p