Data - Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems

<p><strong>Overview</strong></p> <p>The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems". </p> <p><strong>Info</strong></p> <p>ANOVA_Turkey_Sub.R <- code for ANOVA analysis in R statistic 3.3.3    <br> glm.R <- code for GLM analysis in R statistic 3.3.3<br> K&REC_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis<br> K&REC_LPS_PEG_NaPA_K.docx <- File with ANOVA result of partition coefficient (K) for GFP<br> K&REC_LPS_PEG_NaPA_REC.docx <- File with ANOVA result of recover (REC) for GFP         <br> K_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    <br> K_GFP_Pol_005.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  <br> K_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  <br> K_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays  <br> K_GFP_Pol_025.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  <br> K_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays         <br> REC_GFP_Pol_005.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays         <br> REC_GFP_Pol_005.doc  <- File with GLM analysis of GFP recover (REC) in 0.05M salt assays    <br> REC_GFP_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   <br> REC_GFP_Pol_025.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays          <br> REC_GFP_Pol_025.doc  <- File with GLM analysis of GFP recover (REC) in 0.25M salt assays <br> REC_GFP_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays        <br> REM_LPS_PEG_NaPA.docx  <- File with ANOVA result of LPS removal          <br> REM_LPS_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis<br> Stability_GFP_PEG_NaPA.docx <- File with ANOVA result of GFP stability<br> Stability_GFP_PEG_NaPA.xlsx <- File with raw values organized in a spreadsheet of GFP stability results for ANOVA analysis</p> <p>REM_LPS_Pol_005.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays         <br> REM_LPS_Pol_005.doc  <- File with GLM analysis of LPS removal (REM) in 0.05M salt assays    <br> REM_LPS_Pol_005_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.05M salt assays  <br> REM_LPS_Pol_025.csv  <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays         <br> REM_LPS_Pol_025.doc   <- File with GLM analysis of LPS removal (REM) in 0.25M salt assays    <br> REM_LPS_Pol_025_QQ.png <- Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p>K_GFP_Pol_025_NaCl_Li2SO4.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays comparing NaCl and Li2SO4 effect <br> K_GFP_Pol_025_NaCl_Li2SO4.doc  <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays comparing NaCl and Li2SO4 effect <br> K_GFP_Pol_025_NaCl_Li2SO4_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays comparing NaCl and Li2SO4 effect   </p> <p>REM_LPS_Pol_KI_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KI assays comparing salt concentration effect  <br> REM_LPS_Pol_KI_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KI assays comparing salt concentration effect<br> REM_LPS_Pol_KI_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KI assays comparing salt concentration effect<br> REM_LPS_Pol_KNO3_0.05_vs_0.25.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in KNO3 assays comparing salt concentration effect  <br> REM_LPS_Pol_KNO3_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in KNO3 assays comparing salt concentration effect<br> REM_LPS_Pol_KNO3_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in KNO3 assays comparing salt concentration effect<br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in Li2SO4 assays comparing salt concentration effect  <br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in Li2SO4 assays comparing salt concentration effect<br> REM_LPS_Pol_Li2SO4_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in Li2SO4 assays comparing salt concentration effect<br> REM_LPS_Pol_NaCl_0.05_vs_0.25.csv <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in NaCl assays comparing salt concentration effect  <br> REM_LPS_Pol_NaCl_0.05_vs_0.25.doc <- File with GLM analysis of GFP partition coefficient (K) in NaCl assays comparing salt concentration effect <br> REM_LPS_Pol_NaCl_0.05_vs_0.25_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in NaCl assays comparing salt concentration effect</p> <p> </p> <p><strong>Annotation</strong></p> <p>12/12 - Concentration of 12% of each polymer PEG/NaPA</p> <p>16/16 - Concentration of 16% of each polymer PEG/NaPA</p> <p>P/N -  PEG/NaPA</p> <p>10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL</p> <p>poly - Polymer</p> <p>salt - Salt concentration in the assay</p> <p>tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)</p> <p>lps - lipopolysaccharide</p> <p>K - GFP partition coefficient</p> <p>REM - LPS removal</p> <p>REC - GFP recover</p> <p>wo_salt - Assay without salt addition</p> <p><strong>Acknowledgements</strong></p> <p>The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).</p> <p><strong>Consider citing our work. </strong></p> <p>1. Work in progress...</p

Data - Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems

<p><strong>Overview</strong></p> <p>The production of recombinant biopharmaceuticals is highly dependent of a proper choice of the downstream processing stages. Particularly, the purification that must ensure that all the endotoxins (lipopolysaccharide - LPS) are efficiently removed from the final product. This dataset contains the raw data and statistical analysis for the research entitled - "Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems". </p> <p><strong>Info</strong></p> <p>ANOVA_Turkey_Sub.R <- code for ANOVA analysis in R statistic 3.3.3    <br> glm.R <- code for GLM analysis in R statistic 3.3.3<br> K&REC_ORG_ANOVA.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) and recover (REC) for ANOVA analysis</p> <p>K_ORG_ANOVA.docx <-  File with ANOVA result of partition coefficient (K) for GFP</p> <p>REC_ORG_ANOVA.docx  <- File with ANOVA result of partition coefficient (REC) for GFP</p> <p>REM_LPS_ORG_ANOVA.csv  <- File with raw values organized in a spreadsheet of LPS removal for ANOVA analysis</p> <p>REM_LPS_ORG_ANOVA.docx  <- File with ANOVA result of removal of LPS</p> <p>Stability__ORG_ANOVA.csv <- File with raw values organized in a spreadsheet of GFP stability for ANOVA analysis</p> <p>Stability__ORG_ANOVA.docx  <- File with ANOVA result of GFP stability</p> <p>K_ORG_glm_005.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.05M salt assays    </p> <p>K_ORG_glm_005.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.05M salt assays  </p> <p>K_ORG_glm_005_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.05M salt assays  </p> <p>K_ORG_glm_025.csv  <- File with raw values organized in a spreadsheet of GFP partition coefficient (K) for GLM analysis in 0.25M salt assays    </p> <p>K_ORG_glm_025.doc <- File with GLM analysis of GFP partition coefficient (K) in 0.25M salt assays  </p> <p>K_ORG_glm_025_QQ.png <- Residual quantile plot of GLM analysis for partition coefficient (K) in 0.25M salt assays  </p> <p>REC_ORG_glm_005.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.05M salt assays</p> <p>REC_ORG_glm_005.doc <-  File with GLM analysis of GFP recover (REC) in 0.05M salt assays    </p> <p>REC_ORG_glm_005_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.05M salt assays   </p> <p>REC_ORG_glm_025.csv <- File with raw values organized in a spreadsheet of GFP recover (REC) for GLM analysis in 0.25M salt assays</p> <p>REC_ORG_glm_025.doc <-  File with GLM analysis of GFP recover (REC) in 0.25M salt assays    </p> <p>REC_ORG_glm_025_QQ.png <- Residual quantile plot of GLM analysis of GFP recover (REC) in 0.25M salt assays   </p> <p>REM_ORG_glm_005.csv <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.05M salt assays</p> <p>REM_ORG_glm_005.doc <-  File with GLM analysis of LPS removal (REM) in 0.05M salt assays  </p> <p>REM_ORG_glm_005_QQ.png <-  Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p>REM_ORG_glm_025.csv <- File with raw values organized in a spreadsheet of LPS removal (REM) for GLM analysis in 0.25M salt assays</p> <p>REM_ORG_glm_025.doc <-  File with GLM analysis of LPS removal (REM) in 0.25M salt assays </p> <p>REM_ORG_glm_025_QQ.png <-  Residual quantile plot of GLM analysis of LPS removal (REM) in 0.25M salt assays</p> <p> </p> <p><strong>Annotation</strong></p> <p>12/12 - Concentration of 12% of each polymer PEG/NaPA</p> <p>16/16 - Concentration of 16% of each polymer PEG/NaPA</p> <p>P/N -  PEG/NaPA</p> <p>10e4, 10e5, 10e6 - Concentration of LPS in scientific notation - 10000, 100000, 100000 EU/mL</p> <p>poly - Polymer</p> <p>salt - Salt concentration in the assay</p> <p>tsalt - Type of salt in the assay (NaCl, KNO3, KI and Li2SO4)</p> <p>lps - lipopolysaccharide</p> <p>K - GFP partition coefficient</p> <p>REM - LPS removal</p> <p>REC - GFP recover</p> <p>wo_salt - Assay without salt addition</p> <p><strong>Acknowledgements</strong></p> <p>The authors are grateful for financial support from FAPESP (São Paulo Research Foundation, Brazil) through the following projects: 2005/60159-7; 2007/51978-0; 2014/16424-7; and 2014/19793-3. The authors also acknowledge the support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) through the process #0366/09-9 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).</p> <p><strong>Consider citing our work. </strong></p> <p>1. Work in progress...</p