Isolation and purification of Cu-free methanobactin from Methylosinus trichosporium OB3b

<p>Abstract</p> <p>Background</p> <p>The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from <it>Methylosinus trichosporium </it>OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS.</p> <p>Results</p> <p>The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements, and UV-vis spectra indicated that methanobactin was only present in one of the HPLC fractions. On a mass basis, methanobactin carbon contributed only 32% to the total organic carbon isolated with resin extraction. Our spectroscopic results implied that besides methanobactin, the organic compounds in the resin extract comprised breakdown products of methanobactin as well as polysaccharide-like substances.</p> <p>Conclusion</p> <p>Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin. The proposed HPLC purification procedure is suitable for semi-preparative work and provides copper-free methanobactin.</p

Report on a Plus-Energy District with Low-Temperature DHC Network, Novel Agrothermal Heat Source, and Applied Demand Response

District heating and cooling networks can pose the possibility of including a variety of renewable energy sources as well as waste heat into a district&rsquo;s heat supply concept. Unfortunately, low demand densities as they increasingly occur through higher building energy standards and in rural areas render conventional heating and cooling networks inefficient. At the same time, power-to-heat is becoming more and more important to make use of a larger amount of renewable energy sources on the electrical side by providing more flexibility by means of demand response and demand-side management. Within this work, a rural Plus-Energy settlement is presented addressing those topics by a low-temperature district heating and cooling network connected to a novel agrothermal collector supplying 23 residential buildings with decentralized heat pumps and PV systems. The collector, the network, and six of the buildings are equipped with comprehensive monitoring equipment. Within those buildings, forecast and optimization algorithms are implemented to adapt their heat pump operation to enable an increase of self-consumption, to include flexible electricity tariffs, and also to participate in power markets. Thereby, for the low-temperature district heating and cooling network, it has been shown that the concept can operate in the future at competitive heat costs. On the building level, up to 50% of cost savings could be achieved under ideal conditions with the optimization of the self-consumption of PV electricity. However, to ensure optimal results, the individual system components have to be dimensioned for this task

Technological issues to supply low temperature district heating

Current research and development shows that it is technical feasible to change high/medium temperature district heating system to low temperature district heating system for both new and existing building areas. We collect and identify promising technologies in low-temperature district heating application to meetthe goals of future renewable based community energysystems. Innovative technologiesand advancedsystem concepts in low temperature district heating aredescribed. Technological issues to supply lowtemperature district heating to existing building area,supply low temperature district heating with renewablesand supply ultra-low temperature district heating arediscussed in this paper