Overview of the published studies on IGRA response among patients with non tuberculous mycobacterial disease.

#<p>1 co-infected with MAC and <i>M. abscessus</i>.</p><p>*5 patients are excluded due to indeterminate results, 17.5% had a previous history of TB</p><p>+31% had a previous history of TB.</p>##<p>Patients with a history of TB were excluded</p><p>**Patients with a history of TB and TB contacts excluded.</p><p>++4 Patients were excluded due to indeterminate results.</p>###<p>Study of patients suspected of TB.</p><p>***7 Patients were excluded due to indeterminate results.</p><p>+++Data on these patients are not included in the “Total” column below. Age is expressed as median. “Others” constitute <i>M. abscessus/chelonae, interjectum, shimoidei,simiae and xenop</i>i (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-t003" target="_blank">Table 3a</a>).</p><p>Abbreviations: N.A.: not available.</p><p>QTF-2G: QuantiFERON TB 2 Gold, Cellestis.</p><p>QTF-GIT: QuantiFERON TB Gold-In-Tube, Cellestis.</p><p>IGRA: Interferon-gamma release assay.</p><p>MAC: Mycobacterium avium-intracellulare complex.</p><p>T-SPOT: T-SPOT.TB, Oxford Immunotec.</p

Baseline characteristics of persons with a positive NTM culture.

¤<p>n = 55 persons excluded, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-g001" target="_blank">Figure 1</a>.</p><p>*Comparison between “NTM and QFT” and “NTM, no QFT”.</p><p>**Mann-Withney U test.</p><p>***Chi squared.</p><p>****In this category persons with more than 1 NTM species isolated are included (n = 8).</p><p>Total percentages add up to 99–101 because of rounding.</p><p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-t001" target="_blank">Table 1</a> shows the distribution of NTMs isolated in the different groups of participants with or without a matching QFT.</p

Flowchart study population.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-g001" target="_blank">Figure 1</a> illustrates the inclusion and exclusion of persons with positive NTM culture and the classification of patients into three categories on the basis of microbiological data: definite NTM disease, possible NTM disease and NTM colonization.</p

QFT-performance in persons with positive NTM culture (n = 112).

<p>*<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-t003" target="_blank">Table 3</a>.</p><p>**5 with MAC, 3 with <i>M. gordonae</i> and 2 with <i>M. xenopi</i>.</p><p>***2 with <i>M. abscessus-chelonae</i>, 9 with MAC, 2 with <i>M. fortuitum</i>, 35 with <i>M</i>. <i>gordonae</i> and 1 with <i>M. malmoense</i>.</p><p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093986#pone-0093986-t002" target="_blank">Table 2</a> shows the QFT results in patients with positive NTM culture divided into 3 groups according to clinical significance.</p

Additional file 1: of Does an 8-week home-based exercise program affect physical capacity, quality of life, sick leave, and use of psychotropic drugs in patients with pulmonary embolism? Study protocol for a multicenter randomized clinical trial

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Flowchart of children included in the study.

<p>Flowchart of children included in the study.</p

Correlation between IP-10 detected in plasma and DPS (A), and between IP-10 detected in plasma and INF-γ detected in plasma (B).

<p>A total of 25 healthy donors were compared. Nil, CMV-antigen and mitogen samples were pooled, rendering a total of 75 data-points per graph (Spearman).</p

Agreement between IP-10 (A) and IFN-γ (B) detected in plasma and DPS samples.

<p>IP-10 and IFN-γ signals were detected in samples from 18 Quantiferon-TB positive donors; nil, antigen and mitogen samples were corrected for the dilution factor in plasma. Samples were pooled rendering a total of 54 data-points per graph. DPS concentrations were analyzed as ng/2 discs for IP-10 and IU/2 discs for IFN-γ signals (DPS) and log transformed to facilitate comparison between high (plasma) and low DPS. Each sample is represented on the graph as the average of the two measurements against the difference. The limits of agreement are specified as the average difference (bias) ±1.96 standard deviation of the difference.</p

IP-10 responses in DBS compared to DPS.

<p>QFT-CMV test stimulated blood from 8 healthy individuals, 5 CMV responders, were used to compare IP-10 signals in DBS and DPS samples. Nil antigen and mitogen samples pooled (n = 24). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039228#pone-0039228-g004" target="_blank">Figure 4A</a>. Correlation analysis, (p<0.0001, Spearman); <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039228#pone-0039228-g004" target="_blank">Figure 4B</a> Bland-Altman plot of the same data. Each sample is represented on the graph as the average of the two measurements against the difference. The limits of agreement are specified as the average difference (bias, −0.8 pg/ml) ±1.96 standard deviation of the difference, (71 pg/ml).</p

IP-10 responses in plasma and DPS.

<p>QFT-CMV responses from 26 healthy individuals (Control group 2) were divided into CMV-positive (n = 17) and CMV-negative (n = 9) based on the QFT-CMV cut off for IFN-γ suggested by the manufacturer (0.2 IU/ml).</p