Coherent diffraction of thermal currents in Josephson tunnel junctions

We theoretically investigate heat transport in temperature-biased Josephson tunnel junctions in the presence of an in-plane magnetic field. In full analogy with the Josephson critical current, the phase-dependent component of the heat flux through the junction displays coherent diffraction. Thermal transport is analyzed in three prototypical junction geometries highlighting their main differences. Notably, minimization of the Josephson coupling energy requires the quantum phase difference across the junction to undergo \pi-slips in suitable intervals of magnetic flux. An experimental setup suited to detect thermal diffraction is proposed and analyzed.Comment: 6.5 pages, 4 color figures, updated versio

Kinematics of disk galaxies in (proto-)clusters at z=1.5

We observed star-forming galaxies at z~1.5 selected from the HyperSuprimeCam Subaru Strategic Program. The galaxies are part of two significant overdensities of [OII] emitters identified via narrow-band imaging and photometric redshifts from grizy photometry. We used VLT/KMOS to carry out Halpha integral field spectroscopy of 46 galaxies in total. Ionized gas maps, star formation rates and velocity fields were derived from the Halpha emission line. We quantified morphological and kinematical asymmetries to test for potential gravitational (e.g. galaxy-galaxy) or hydrodynamical (e.g. ram-pressure) interactions. Halpha emission was detected in 36 targets. 34 of the galaxies are members of two (proto-)clusters at z=1.47, confirming our selection strategy to be highly efficient. By fitting model velocity fields to the observed ones, we determined the intrinsic maximum rotation velocity Vmax of 14 galaxies. Utilizing the luminosity-velocity (Tully-Fisher) relation, we find that these galaxies are more luminous than their local counterparts of similar mass by up to ~4 mag in the rest-frame B-band. In contrast to field galaxies at z<1, the offsets of the z~1.5 (proto-)cluster galaxies from the local Tully-Fisher relation are not correlated with their star formation rates but with the ratio between Vmax and gas velocity dispersion sigma_g. This probably reflects that, as is observed in the field at similar redshifts, fewer disks have settled to purely rotational kinematics and high Vmax/sigma_g ratios. Due to relatively low galaxy velocity dispersions (sigma_v < 400 km/s) of the (proto-)clusters, gravitational interactions likely are more efficient, resulting in higher kinematical asymmetries, than in present-day clusters. (abbr.)Comment: Accepted for publication in A&A. 11 pages, 8 figures, 1 tabl

Characterization of LR-115 Type 2 Detectors for Monitoring Indoor Radon 222: Determination of the Calibration Factor

The city of Lima, capital of Peru, has about 11 million inhabitants. Lima has no records about the indoor Radon 222 concentration levels in dwellings. Hereby, we are planning to register the indoor radon concentrations in Lima and in other cities of Peru in the next three years. First, we will determine the calibration factor for the detectors which will be used in our measurements. For this purpose, Solid State Nuclear Tracks Detectors of nitrocellulose nitrate (LR-115 type 2) were used.The calibration process using a Radium 226 source was described to obtain the calibration factor. Linear response in tracks number was found in relation with irradiation time and its stability after time at the calibration chamber

Focused directed evolution of aryl-alcohol oxidase in Saccharomyces cerevisiae by using chimeric signal peptides

Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the lack of suitable heterologous expression systems has precluded its engineering by directed evolution. In this study, the native signal sequence of AAO from Pleurotus eryngii was replaced by those of the mating a-factor and the K1 killer toxin, as well as different chimeras of both prepro-leaders in order to drive secretion in Saccharomyces cerevisiae. The secretion of these AAO constructs increased in the following order: preproa-AAO> preaproK-AAO>preKproa-AAO>preproK-AAO. The chimeric preaproK-AAO was subjected to focused-directed evolution with the aid of a dual screening assay based on the Fenton reaction. Random mutagenesis and DNA recombination was concentrated on two protein segments (Meta1]-Val109 and Phe392-Gln566), and an array of improved variants was identified, among which the FX7 mutant (harboring the H91N mutation) showed a dramatic 96-fold improvement in total activity with secretion levels of 2 mg/liter. Analysis of the N-terminal sequence of the FX7 variant confirmed the correct processing of the preaproK hybrid peptide by the KEX2 protease. FX7 showed higher stability in terms of pH and temperature, whereas the pH activity profiles and the kinetic parameters were maintained. The Asn91 lies in the flavin attachment loop motif, and it is a highly conserved residue in all members of the GMC superfamily, except for P. eryngii and P. pulmonarius AAO. The in vitro involution of the enzyme by restoring the consensus ancestor Asn91 promoted AAO expression and stability

Optimization of the Multi-Spectral Euclidean Distance Calculation for FPGA-based Spaceborne Systems

Due to the high quantity of operations that spaceborne processing systems must carry out in space, new methodologies and techniques are being presented as good alternatives in order to free the main processor from work and improve the overall performance. These include the development of ancillary dedicated hardware circuits that carry out the more redundant and computationally expensive operations in a faster way, leaving the main processor free to carry out other tasks while waiting for the result. One of these devices is SpaceCube, a FPGA-based system designed by NASA. The opportunity to use FPGA reconfigurable architectures in space allows not only the optimization of the mission operations with hardware-level solutions, but also the ability to create new and improved versions of the circuits, including error corrections, once the satellite is already in orbit. In this work, we propose the optimization of a common operation in remote sensing: the Multi-Spectral Euclidean Distance calculation. For that, two different hardware architectures have been designed and implemented in a Xilinx Virtex-5 FPGA, the same model of FPGAs used by SpaceCube. Previous results have shown that the communications between the embedded processor and the circuit create a bottleneck that affects the overall performance in a negative way. In order to avoid this, advanced methods including memory sharing, Native Port Interface (NPI) connections and Data Burst Transfers have been used