Evaluation of the cell viability of human Wharton's jelly stem cells for use in cell therapy

Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity. We employed a combination of highly sensitive techniques to determine the average cell viability levels and proliferation capabilities of 10 consecutive cell passages of cultured HWJSCs and then used RNA microarrays to identify genes associated with changes in cell viability levels. We found an initial decrease in cell viability from the first to the third cell passage followed by an increase until the sixth passage and a final decrease from the sixth to tenth cell passages. The highest cell viability levels corresponded to the fifth and sixth passages. The intracellular ionic contents of potassium, sodium, and chlorine suggest that the lower cell viability levels at passages 2, 3, and 8-10 may be associated with apoptotic cell death. In fact, gene expression analysis revealed that the average cell viability was significantly associated with genes with a function in apoptotic cell death, especially pro-apoptotic FASTKD2, BNIP3L genes and anti-apoptotic TNFAIP8 and BCL2L2 genes. This correlation with both pro-apoptotic and anti-apoptotic genes suggests that there may be a complex live-death equilibrium in cultured HWJSCs kept in culture for multiple cell passages. In this study, the highest cell viability levels corresponded to the fifth and sixth HWJSC passages, suggesting that these passages should be preferentially employed in cell therapy or tissue engineering protocols using this cell type

INCIDENCES OF STIFFNESS ON SQUAT JUMP VARIABLES

The purpose of this study was to relation level the stiffness of the patellar tendon and three portions of the Achilles tendon; inferior, middle, and superior location with performance variables in the squat jump. The sample was composed of 25 belonging to Chilean elite male handball players. It assesses stiffness tendinous patellar and Achilles tendon using to MyotonPro® device. During the squat jump assessment, two force platforms Pasco and ForceDecks® software for analysis. The main results were RFD 0-50 ms (r=0.611), RFDN 0-50 ms (r=0.550), RFD 0-100 ms (r=0.615), and RFDN 0-100 ms (r=0.624) presented a relationship with patellar stiffness, while that Achilles tendon was down relation with peak power in squat jump (r=472). This information can be useful for training judo athletes. From the results, it can be concluded that the stiffness of the patellar tendon and the Achilles tendon determine some mechanical variables of the SJ, mainly in relation to the RFD