Dictionary Learning-based Inpainting on Triangular Meshes

The problem of inpainting consists of filling missing or damaged regions in images and videos in such a way that the filling pattern does not produce artifacts that deviate from the original data. In addition to restoring the missing data, the inpainting technique can also be used to remove undesired objects. In this work, we address the problem of inpainting on surfaces through a new method based on dictionary learning and sparse coding. Our method learns the dictionary through the subdivision of the mesh into patches and rebuilds the mesh via a method of reconstruction inspired by the Non-local Means method on the computed sparse codes. One of the advantages of our method is that it is capable of filling the missing regions and simultaneously removes noise and enhances important features of the mesh. Moreover, the inpainting result is globally coherent as the representation based on the dictionaries captures all the geometric information in the transformed domain. We present two variations of the method: a direct one, in which the model is reconstructed and restored directly from the representation in the transformed domain and a second one, adaptive, in which the missing regions are recreated iteratively through the successive propagation of the sparse code computed in the hole boundaries, which guides the local reconstructions. The second method produces better results for large regions because the sparse codes of the patches are adapted according to the sparse codes of the boundary patches. Finally, we present and analyze experimental results that demonstrate the performance of our method compared to the literature

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data