Effects of discrete-electrode configuration on traveling-wave electrohydrodynamic pumping

Traveling-wave electrohydrodynamic (EHD) micropumps can be incorporated into the package of an integrated circuit chip to provide active cooling. They can also be used for fluid delivery in microdevices. The pump operates in the presence of a thermal gradient through the fluid layer such that a gradient in electrical conductivity is established allowing ions to be induced. These ions are driven by a traveling electric field. Such a traveling electric field can be realized in practice only via discrete electrodes upon which the required voltages are imposed. The impact of using discrete electrodes to create the traveling wave on the flow rates generated is explored through numerical modeling. The change in performance from an ideal sinusoidal voltage boundary condition is quantified. The model is used to explore the widths of electrodes and the intervening isolation regions that lead to optimized pumping. The influence of the choice of working fluid on the performance of the pump is determined using an analytical model

Direct Quantification of Campylobacter jejuni in Chicken Fecal Samples Using Real-Time PCR: Evaluation of Six Rapid DNA Extraction Methods

Direct and accurate quantification of Campylobacter in poultry is crucial for the assessment of public health risks and the evaluation of the effectiveness of control measures against Campylobacter in poultry. The aim of this study was to assess several rapid DNA extraction methods for their effectiveness for the direct quantification (without enrichment) of Campylobacter jejuni in chicken fecal samples using real-time PCR. The presence of inhibitory substances in chicken fecal samples may reduce or even completely impede the PCR amplification process making quantification very difficult. Six rapid DNA extraction methods were compared based on their limit of detection, efficiency, reproducibility, and precision. Standard curves were designed for all the methods tested in order to assess their performance on the direct quantification of C. jejuni in chicken fecal samples. As a result of this study, the Easy-DNA (Invitrogen) method generated lower Ct values, the best amplification efficiency (AE = 93.2 %) and good precision (R squared = 0.996). The method NucleoSpin® Tissue was able to detect samples spiked with the lowest Campylobacter concentration level (10 CFU/ml) but the amplification efficiency was not optimal (AE = 139.5 %). DNA extraction methods Easy-DNA Invitrogen, MiniMAG® and NucleoSpin® Tissue produced good real-time PCR reproducibility generating standard deviations from 0.3 to 0.8 between replicates