Differences in the CTCE migration times of all peaks relative to the most thermally stable homoduplex for target sequences 1–12

<p><b>Copyright information:</b></p><p>Taken from "Technology to accelerate pangenomic scanning for unknown point mutations in exonic sequences: cycling temperature capillary electrophoresis (CTCE)"</p><p>http://www.biomedcentral.com/1471-2156/8/54</p><p>BMC Genetics 2007;8():54-54.</p><p>Published online 14 Aug 2007</p><p>PMCID:PMC2042502.</p><p></p> Five four-minute cycles (20 min) were employed with an amplitude of 12°C (47°C – 59°C). The results are illustrated as the average migration time difference +/- 1 standard deviation, n = 8. A representative electropherogram obtained from fragment #6 is incorporated to illustrate peak positions

The melting profile of three target sequences 6, 14 and 26 calculated with WinMelt illustrating a well-defined target of a single isomelting domain (6), a target with two isomelting domains (26) and a target with an irregular melting profile (16)

<p><b>Copyright information:</b></p><p>Taken from "Technology to accelerate pangenomic scanning for unknown point mutations in exonic sequences: cycling temperature capillary electrophoresis (CTCE)"</p><p>http://www.biomedcentral.com/1471-2156/8/54</p><p>BMC Genetics 2007;8():54-54.</p><p>Published online 14 Aug 2007</p><p>PMCID:PMC2042502.</p><p></p> The symbols mark the position of the sequence differences AT>GC (#6), TA>CG (#14) and TA>CG (#26) in each target wild type>mutant pair in separation trials. The GC-clamp (~94°C) is not shown but was incorporated in the melting calculations attached to the higher melting temperature end of each target sequence