Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil.

In this study, we develop a competitive ratiometric fluorescent lateral flow immunoassay (CRF-LFIA) based on dual emission fluorescence signal, which has great advantage in visual and quantitative detection of Chlorothalonil (CTN). Red-emitted fluorescent magnetic nanobeads (FMNBs) and green-emitted aggregation-induced emission fluorescent microsphere (AIEFM) are synthesized and conjugated to antibodies and antigens respectively, resulting in competitive binding with the analyte. The ratiometric fluorescence signal which comes from the overlap of these two fluorescence emissions. FMNBs probes also provide immunomagnetic separation (IMS) to enrich the analysts and resist complex matrix effects. This strip generates a visually discernible yellow-to-green fluorescence color change in the presence of CTN (2 ng/mL), which could be incisively observed by naked eye. Moreover, the limit of detection (LOD) reached 0.152 ng/mL by measurement of color (Red-Green-Blue, RGB) signals. Method validation shows a good correlation between CRF-LFIA and LC-MS/MS

Laser powder bed fusion processed LaCe(Fe, Mn, Si)₁₃ lattices for magnetic refrigeration:Process optimization, microstructure, and magnetocaloric performance

In this study, the optimal laser powder bed fusion (LPBF) processing parameters for fabricating fully dense LaCe(Fe,Mn,Si)13 thin walls have been identified through a machine-learning approach based on the Gaussian process regression (GPR) model. All the specimens and components were fabricated using a continuous laser source. The relationship between the defect fraction of the fabricated thin wall and the line energy density (EL) and hatch (h) is established. The measured defect fraction of specimens fabricated using the validation data sets was very well in agreement with the predicted values of the GPR model, with an error of less than 1%. The microstructure of as-fabricated lattices is contained by α-Fe phases, LaFeSi phases, NaZn13-type phases, and the La/Ce/Si rich phases, which has an amorphous matrix embedded with nanocrystalline. The microstructure of the HTHed lattices presents the α-Fe phases, LaFeSi phases, and NaZn13-type phases. The diamond lattice has high heat exchange efficiency among the four lattices because of its large surface area (1577.1 mm2) and excellent thermal conductivity. Although the LPBF parameters are the same, the maximum isothermal magnetic entropy change (ΔSm) and Tc of the four samples differ. The X-ray powder diffraction test confirms that the HTHed Tube exhibits the highest volume of the NaZn13-type phase. The HTHed Tube saw ΔSm, about 1.63 J kg−1K−1 at 264.5 K. The ΔSm of HTHed Diamond around Tc (1.13 J kg−1K−1 at 219.5 K) is slightly higher than HTHed Gyroid (0.99 J kg−1K−1 at 250.5 K). Essentially, our work accelerates the search for optimal process parameters and guides the direction for lattice design of LaCe(Fe,Mn,Si)13.</p

Human Ergonomics Study in Microgravity Environment

In recent decades, human exploration of space has gradually deepened, and more and more countries and regions have successfully sent astronauts into space. How to complete the space experiment as efficiently and safely as possible in the shortest time with limited resources has become an important issue in the field of aerospace technology. Taking full account of such matter, we consider that ergonomics in design stage is one of the most effective ways to solve this problem. The microgravity environment is the biggest difference between ground and space. Therefore, the study of ergonomics under the microgravity environment is of great significance. This article deeply analyses and summarizes the physiological differences between human and normal gravity under microgravity environment. From the ergonomics point of view, the requirements for spacecraft design and space mission planning are put forward for the reference of engineers and scholars

Human Ergonomics Study in Microgravity Environment

In recent decades, human exploration of space has gradually deepened, and more and more countries and regions have successfully sent astronauts into space. How to complete the space experiment as efficiently and safely as possible in the shortest time with limited resources has become an important issue in the field of aerospace technology. Taking full account of such matter, we consider that ergonomics in design stage is one of the most effective ways to solve this problem. The microgravity environment is the biggest difference between ground and space. Therefore, the study of ergonomics under the microgravity environment is of great significance. This article deeply analyses and summarizes the physiological differences between human and normal gravity under microgravity environment. From the ergonomics point of view, the requirements for spacecraft design and space mission planning are put forward for the reference of engineers and scholars

Negative correlation between latent Epstein-Barr virus infection and severity of illness in IBD patients

Abstract Background: Inflammatory bowel (IBD) disease plays an important role in the prevention and disease management. Immunosuppressive therapies have been found to induce IBD pathogenesis and reactivate latent Epstein-Barr virus (EBV) . This study was aimed to explore the clinical significance of latent EBV infection in IBD patients. Methods: Latent EBV infection was determined by double staining for EBV encoded RNA (EBER) and CD20 in colon specimens of 43 IBD patients who underwent bowel resection. Based on the staining results, the patients were divided into two groups, according to their latent EBV infection states - negative (n=33) and positive (n=10). The clinic-pathological data were analyzed between the two different latent EBV groups and also between the mild-to-moderate and severe disease groups.Results: Systolic pressure (P=0.005), variety of disease (P=0.005), the severity of illness (P=0.002), and pre-op corticosteroids (P=0.025) were significantly different between the EBV-negative and EBV-positive groups. Systolic pressure (P=0.001), variety of disease (P=0.000), pre-op corticosteroids (P=0.011) and EBV infection (P=0.003) were significantly different between the mild-to-moderate and severe disease groups.Conclusions: IBD patients with latent EBV infection may manifest more severe illnesses. It is suggested that the role of EBV in IBD development should be further investigated and latent EBV infection in patients with serious IBD should be closely monitored, and therapeutic course should be optimized.</jats:p