10 research outputs found
Multivariate logistic regression analysis of the variables (Forward conditional).
<p>Multivariate logistic regression analysis of the variables (Forward conditional).</p
Comparison of 12 miRNAs expression levels between sepsis non-survivors and survivors in a validation set.
<p>Differentially expressed miRNAs identified by Solexa sequencing were validated by qRT-PCR in sepsis survivors (S; N = 15) compared to non-survivors (D; N = 15). MiR-16 (<i>p = 0.005</i>), miR-15a (<i>p = 0.021</i>), miR-223 (<i>p = 0.015</i>), miR-483-5p (<i>p<0.001</i>), miR-193b* (<i>p<0.001</i>), miR-122 (<i>p<0.001</i>), and miR-499-5p (<i>p = 0.006</i>) were significantly different after validation with qRT-PCR. Expression levels of the 12 miRNAs were normalized to U6 snRNA above normal controls and given as fold-changes (2<sup>–ΔΔCt</sup> ). △△Ct  =  (Ct<sub>miRNA</sub>-Ct<sub>U6 </sub><sub>snRNA</sub>) <sub>patients</sub>-(Ct<sub>miRNA</sub>-Ct<sub>U6 snRNA</sub>)<sub>controls</sub>. Mann-Whitney U-test was used for statistical comparisons.</p
Receiver operating characteristic (ROC) curves for serum miRNAs for sepsis non-survivors (n = 73) and survivors (n = 93).
<p>These six miRNAs were finally confirmed by qRT-PCR. Areas under the ROC curves are also shown.</p
Fold-changes of selected miRNAs in Non-survivors (D) and Survivors (S).
*<p>Fold change formula: Fold-change = log2 (D/S).</p
Expression levels of 8 miRNAs in sepsis non-survivors and survivors in a confirmation set.
<p>These 8 miRNAs were significantly differentially expressed between sepsis non-survivors and survivors after qRT-PCR validation in a smaller study sample. Then, these were checked by qRT-PCR in a larger study sample size (Non-survivors = D, n = 73; Survivors = S, n = 93). Only 6 of the 8 miRNAs remained as significantly different between the D group and S groups. Expression levels of these 8 miRNAs were normalized to U6 snRNA U6 snRNA above normal controls and given as fold-changes (2<sup>–ΔΔCt</sup> ), △△Ct =  (Ct<sub>miRNA</sub>-Ct<sub>U6 </sub><sub>snRNA</sub>)<sub>patients</sub>-(Ct<sub>miRNA</sub>-Ct<sub>U6 snRNA</sub>)<sub>controls</sub>. Mann-Whitney U-test or student t-test was used for statistical comparisons.</p
Receiver operating characteristic (ROC) curves for serum miRNAs and clinically used indicators for sepsis non-survivors (n = 73) and survivors (n = 93).
<p>Serum levels of miRNAs were quantified using real time qPCR. Each qPCR was done in triplicate in 96-well plates. Expression levels of the selected miRNAs were normalized to U6 snRNA and presented as fold-changes (2<sup>-ΔΔCt</sup>) above normal controls.</p
Unveiling the Critical Relationship between MXene Double-Layer Capacitance and Electronic Configuration
MXene, with highly tunable and controllable surface terminations,
is an emerging electrode material for electric double-layer (EDL)
capacitors used in electrochemical energy storage. However, the influence
of alterations in the electronic configuration of MXene induced by
modifications in functional groups on EDL capacitance remains elusive.
Thus, an implicit self-consistent electrolyte model is developed to
investigate the EDL capacitance and structure of Mo2CTx MXene as a function of electronic configuration
at an atomic scale. We reveal a strong correlation between the electronic
configurations of metal Mo in Mo2CTx MXene and its EDL capacitance, with the dz2 orbital of Mo perpendicular to the MXene surface
playing a crucial role. The higher EDL capacitance and thinner EDL
thickness primarily originate from a lower number of occupied electrons
in the d orbitals (higher unoccupied d orbitals) and a larger d-band
occupied center. Furthermore, this relationship can be further extended
to the halogen termination of MXene. Notably, by manipulating the
surface terminations, the electronic configurations (occupied and
unoccupied orbitals) of Mo orbitals can be regulated, thus providing
a facilitative way to control the EDL capacitance. The results show
that the EDL capacitance depends not only on the electrode–electrolyte
interfacial structure but also on the electronic configuration. These
findings provide a solid foundation for regulating the structure and
capacitance of the EDL of MXene from an electronic perspective, which
could have significant implications for the development of advanced
energy storage devices
X‑ray Insights into Formation of −O Functional Groups on MXenes: Two-Step Dehydrogenation of Adsorbed Water
Engineered MXene surfaces with more −O functional
groups
are feasible for realizing higher energy density due to their higher
theoretical capacitance. However, there have been only a few explorations
of this regulation mechanism. Investigating the formation source and
mechanism is conducive to expanding the adjustment method from the
top-down perspective. Herein, for the first time, the formation dynamics
of −O functional groups on Mo2CTx are discovered as a two-step dehydrogenation of adsorbed water
through in situ near-ambient-pressure X-ray photoelectron spectroscopy,
further confirmed by ab initio molecular dynamics simulations. From
this, the controllable substitution of −F functional groups
with −O functional groups is achieved on Mo2CTx during electrochemical cycling in an aqueous
electrolyte. The obtained Mo2CTx with rich −O groups exhibits a high capacitance of 163.2
F g –1 at 50 mV s –1, together
with excellent stability. These results offer new insights toward
engineering surface functional groups of MXenes for many specific
applications
X‑ray Insights into Formation of −O Functional Groups on MXenes: Two-Step Dehydrogenation of Adsorbed Water
Engineered MXene surfaces with more −O functional
groups
are feasible for realizing higher energy density due to their higher
theoretical capacitance. However, there have been only a few explorations
of this regulation mechanism. Investigating the formation source and
mechanism is conducive to expanding the adjustment method from the
top-down perspective. Herein, for the first time, the formation dynamics
of −O functional groups on Mo2CTx are discovered as a two-step dehydrogenation of adsorbed water
through in situ near-ambient-pressure X-ray photoelectron spectroscopy,
further confirmed by ab initio molecular dynamics simulations. From
this, the controllable substitution of −F functional groups
with −O functional groups is achieved on Mo2CTx during electrochemical cycling in an aqueous
electrolyte. The obtained Mo2CTx with rich −O groups exhibits a high capacitance of 163.2
F g –1 at 50 mV s –1, together
with excellent stability. These results offer new insights toward
engineering surface functional groups of MXenes for many specific
applications
Monolayer Thiol Engineered Covalent Interface toward Stable Zinc Metal Anode
Interface engineering of zinc metal anodes is a promising
remedy
to relieve their inferior stability caused by dendrite growth and
side reactions. Nevertheless, the low affinity and additional weight
of the protective coating remain obstacles to their further implementation.
Here, aroused by DFT simulation, self-assembled monolayers (SAMs)
are selectively constructed to enhance the stability of zinc metal
anodes in dilute aqueous electrolytes. It is found that the monolayer
thiol molecules relatively prefer to selectively graft onto the unstable
zinc crystal facets through strong Zn–S chemical interactions
to engineer a covalent interface, enabling the uniform deposition
of Zn2+ onto (002) crystal facets. Therefore, dendrite-free
anodes with suppressed side reactions can be achieved, proven by in
situ optical visualization and differential electrochemical mass spectrometry
(DEMS). In particular, the thiol endows the symmetric cells with a
4000 h ultrastable plating/stripping at a specific current density
of 1.0 mA cm–2, much superior to those of bare zinc
anodes. Additionally, the full battery of modified anodes enables
stable cycling of 87.2% capacity retention after 3300 cycles. By selectively
capping unstable crystal facets with inert molecules, this work provides
a promising design strategy at the molecular level for stable metal
anodes