2 research outputs found
A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M<sub>1</sub> and Pirlimycin Residues in Milk
Mycotoxins and antibacterial agents
are the main chemical hazards
that lead to several health problems. Nowadays, multiplex immunoassay
is a primary goal throughout the world. Here, aflatoxin M<sub>1</sub> and pirlimycin were selected as models, and a novel dual colorimetric
encoded frit-based immunoassay was developed for simultaneously screening
of aflatoxin M<sub>1</sub> and pirlimycin residues in milk. This multiplex
frit-based immunoassay combined two monoclonal antibodies to extend
the spectrum of analytes and to enable detection of two classes of
analytes in a single test. The cutoff values were 0.02 μg/kg
for aflatoxin M<sub>1</sub> and 0.5 μg/kg for pirlimycin, which
satisfied the requirement to measure the maximum residue levels. The
novel colorimetric frit-based immunoassay has the advantage of high
throughput, short analysis time, reduced overall cost per assay, and
can be used as a rapid screening technique for simultaneously detecting
aflatoxin M<sub>1</sub> and pirlimycin residues in milk
Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay
Food
contamination by veterinary drug residues is a worldwide public
health concern and requires continuous monitoring. In this study,
we developed a biotin–streptavidin-amplified ELISA (BA-ELISA)
using a produced monoclonal antibody for detecting pirlimycin residues
in beef muscle, milk, and honey. The IC<sub>50</sub> value of the
BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity
was improved 3 times compared to traditional ELISAs. The optimized
BA-ELISA can be used to quantitate trace amounts of pirlimycin residues
in beef muscle, milk, and honey. This method had limits of detection
(LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk,
and 2.75 μg/kg in honey. The average recovery of the BA-ELISA
ranged from 78 to 97%, and the coefficient of variation ranged from
5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS,
and the BA-ELISA can be used for routine screening analysis of pirlimycin
residues