Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury

Specific biomarkers of ferroptosis after peripheral nerve injury (PNI) are still under debate. In this study, 52 differentially expressed ferroptosis-related genes (DE-FRGs) were retrieved from publicly accessible sequencing data of intact and injured samples of rats with sciatic nerve crush injury. Functional enrichment analyses revealed that adipogenesis, mitochondrial gene sets, and pathways of MAPK, p53, and CD28 family were predominantly engaged in ferroptosis after PNI. Next, Cdkn1a, Cdh1, Hif1a, Hmox1, Nfe2l2, and Tgfb1 were investigated as new ferroptosis-associated hub genes after PNI. Subsequently, clustering correlation heatmap shows six hub genes are linked to mitochondria. The immunofluorescence assay at 0, 1, 4, 7, and 14 days indicated the temporal expression patterns of Tgfb1, Hmox1, and Hif1a after PNI were consistent with ferroptosis validated by PI and ROS staining, while Cdh1, Cdkn1a, and Nfe2l2 were the opposite. In summary, this study identified six hub genes as possible ferroptosis-related biomarkers for PNI, which may offer therapeutic targets for peripheral nerve regeneration and provide a therapeutic window for ferroptosis

The succession of rhizosphere microbial community in the continuous cropping soil of tobacco

Introduction: Flue-cured tobacco is an important economic crop that is not tolerant of continuous cropping and can be influenced by planting soil conditions including rhizosphere microbial communities and soil physicochemical properties. The relationship between rhizosphere microbial communities and soil physicochemical properties under continuous cropping conditions is unclear.Methods: This study investigated the succession of rhizosphere microbial community in continuous tobacco cropping soil for 1, 3, 5, 8, 10, 15, and 30 years. The physicochemical properties of the soil were measured, high-throughput sequencing was performed on the rhizosphere microbial community, and correlation analysis was conducted.Results: The results suggested that continuous cropping could significantly enrich soil available nitrogen, available phosphorus, available potassium, and organic matter. Meanwhile, the alpha diversity of the bacterial community was significantly reduced with continuous cropping, indicating significant changes in the structure of bacterial and fungal communities. Based on linear discriminant analysis effect size (LEfSe), 173 bacterial and 75 fungal genera were identified with significant differences. The bacterial genera, Sphingomonas, Streptomyces, and Microvirga, were significantly positively correlated with continuous cropping years. The fungal genera, Tausonia, Solicocozyma, Pseudomycohila, and Fusarium, also showed significant positive correlation with continuous cropping years. Meanwhile, the fungal genera, Olpidium, Cephaliophora, and Cercophora, presented an opposite correlation. However, there are differences in the correlation between these bacterial and fungal genera related to continuous cropping years and other different soil physicochemical properties.Discussion: In summary, this work could provide a reference for soil management and scientific fertilization of tobacco under continuous cropping conditions

Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors

Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate MYB transcription factor, CsMYB85, that is involved in the regulation of lignin biosynthesis in Citrus sinensis, which has homologs in Arabidopsis and other plants. We found that during juice sac lignification, CsMYB85 expression levels increase significantly, and therefore, suspected that this gene may control lignin biosynthesis during the lignification process. Our results indicated that CsMYB85 binds the CsMYB330 promoter, regulates its expression, and interacts with CsMYB308 in transgenic yeast and tobacco. A transient expression assay indicated that Cs4CL1 expression levels and lignin content significantly increased in fruit juice sacs overexpressing CsMYB85. At4CL1 expression levels and lignin content were also significantly increased in Arabidopsis overexpressing CsMYB85. We accordingly present convincing evidence for the participation of the CsMYB85 transcription factor in fruit juice sac lignification, and thereby provide new insights into the transcriptional regulation of this process in citrus fruits

Revealing the Strain Effect on Radiation Response of Amorphous–Crystalline Cu-Zr Laminate

Nanocrystalline materials containing amorphous intergranular films (AIFs) exhibit excellent mechanical properties, radiation resistance, and thermal stability and may serve as promising candidate materials for use in advanced nuclear energy systems. The aim of this work is to reveal the effect of mechanical stress on the radiation damage behavior of AIF systems. Based on a bicrystal Cu system with Zr-doped AIFs, molecular dynamics is used to simulate the radiation process and examine the AIF sink efficiency, defect propensity, defect size distribution, and Zr mixing under uniaxial and hydrostatic strain conditions. The results show that the sink efficiency of the glue-like AIFs is not compromised under applied strains. The anisotropy resulting from the intrinsic microstructure and elastic deformation leads to a distinct radiation response, where extension (contraction) of the structure perpendicular to the AIFs increases (decreases) the vacancy density. The strain-dependent defect density, along with the cluster size distributions, can be interpreted based on the variations in the defect formation energy and anisotropic defect diffusion. Finally, the Zr mixing induced by collision cascades is found to be insensitive to the mechanical strains. These findings provide meaningful information towards understanding the stress effect on the radiation response of AIF systems

Utilization of UV-Vis spectroscopy and related data analyses for dissolved organic matter (DOM) studies: A review

<p>Despite the popular use of UV-Vis spectroscopy for dissolved organic matter (DOM) studies, many of the derived parameters and the related methods have been rather limitedly used for certain areas of DOM studies, which warrants the extension of their applications into a wider range of research topics as well as critical comparison of individual parameters. In this review, we comprehensively compared the UV-Vis spectroscopy–based DOM parameters widely used for the last few decades, aiming to broaden our knowledge about the utilization of the spectra for DOM studies through bridging the gaps between different research areas (e.g., natural versus engineered systems). The parameters for chromophoric DOM (CDOM) addressed here encompass absorption coefficient, absorption ratios, spectral slopes, derivative and differential spectra, Gaussian decomposition, and two-dimensional correlation analysis. The benefit of using such parameters could be maximized in complementary uses both for static environments and dynamic processes. For the fullest utilization of the spectral potential, it is recommended to open the raw UV-Vis spectra and the measurement conditions (solution chemistry, types of water, wavelengths, derivation methods, etc) to the public, which can enhance global comparison and our understanding of the fate and the distribution of CDOM and the dynamics in various aquatic environments.</p

Thermodynamic mixing energy and heterogeneous diffusion uncover the mechanisms of radiation damage reduction in single-phase Ni-Fe alloys

Understanding and predicting radiation damage is of central importance to develop radiation-tolerant structural materials for current and next-generation nuclear systems. Single-phase solid solution alloys constitute attractive choices due to their promising mechanical properties and radiation tolerance. Here, by examining radiation-induced defect production and evolution in single-phase Ni-Fe alloys, we show that radiation damage resistance directly correlates with thermodynamic mixing energy and heterogeneity of defect diffusion. We found that radiation damage in materials decreases linearly with lowering mixing energy, and the relationship holds true for all studied Ni-Fe compositions. The damage reduction with varying composition is further ascribed to the increasing heterogeneity of point defect migration across a complex potential energy landscape that enhances defect recombination. This new insight into the dynamical evolution of radiation defects points to a thermodynamic criterion for designing radiation-tolerant materials

Evaluation of Resource and Environmental Carrying Capacity of China’s Rapid-Urbanization Areas—A Case Study of Xinbei District, Changzhou

The evaluation of resource and environmental carrying capacity (RECC) is the foundation for the rationale behind the arrangement of land spaces for production, living, and ecological uses. In this study, based on various natural, economic, and social factors, an integrated Multi-Factor assessment model was developed to evaluate the RECC of Xinbei district of Changzhou. Meanwhile, we also calculated the population carrying capacity estimation model restricted by food security. The study comprehensively analyzed the current status and land resource characteristics of a rapid urbanization area and the RECC restrictions for protection and development. The results indicate that the comprehensive carrying capacity of Xinbei showed distinct spatial heterogeneity, with a decreasing trend from the riverside protection area to urban areas, then to mountain areas. Combined with the secure food supply provided by future land resources, it was estimated that the population carrying index of Xinbei would be as high as 1.25 and 1.22 in 2035 and 2050, respectively, indicating that both years would experience a population overload. Therefore, an urgent adjustment to the structure and layout of territorial space and resources of the Xinbei District is necessary

Position Control Study on Pump-Controlled Servomotor for Steam Control Valve

In steam turbine control and actuation, the steam control valve plays a key role in operability and reliability. The electrohydraulic regulating system for the steam control valve, usually called the servomotor, needs to be reliable and high performing under nonlinear excitation interference in actual conditions. Currently, electrohydraulic servo valve control technology is widely used in servomotors. Although this technology has good control performance, it still has some technical defects, such as poor antipollution ability, low energy efficiency, large volume size, and limited installation space. Aiming at the abovementioned technical shortcomings of electrohydraulic servo valve control technology, a servomotor-pump-hydraulic cylinder volume control scheme is proposed in this paper, forming a pump-controlled servomotor for the steam control valve. By analyzing the working principle of the pump-controlled servomotor position control in the steam control valve, the mathematical model of a pump-controlled servomotor for the steam control valve is established. The sliding mode variable structure control strategy is proposed, and the variable structure control law is solved by constructing a switching function. To verify the performance of the proposed control method, experimental research was conducted. The research results show that the proposed sliding mode variable structure control strategy has a good control effect, which lays the theoretical and technical foundation for the engineering application and promotion of pump-controlled servomotors for steam control valves and helps the technical upgrade and product optimization of steam turbines

Mapping Chinese land system types from the perspectives of land use and management, biodiversity conservation and cultural landscape

Land is an integrated and complex system consisting of multiple natural and anthropogenic elements. The long-term exploitation and utilization of land resources by humans have transformed the terrestrial surface of the Earth and profoundly impacted the spatial patterns of regional land use and land cover. Land use/cover (LULC) data have been extensively used to monitor regional land changes and contribute to land use policy making. However, these products objectively reflect only the current coverage and biophysical attributes of the terrestrial surface but fail to characterize the multiple functions and attributes of the human-environment system (HES). In this study, we present an integrated land system classification for representing the regional HES based on multisource datasets that characterize the attributes of land use and management, biodiversity conservation and cultural landscapes. Then, 40 land system types (LSTs) in China were identified using the self-organizing map (SOM) algorithm. According to the land system map, we found significant east–west differentiation in land use intensity, with high-intensive LSTs occurring mostly in eastern provinces and natural and seminatural LSTs dominating in central and western regions. The multifunctional LSTs that host food production, biodiversity conservation and cultural heritage were estimated to cover 32% of the terrestrial area in China. In summary, our results can provide a basis for regional land assessment and help identify the pressures on and threats to the eco-environment. Mapping regional land systems facilitates an in-depth understanding of human-environment interactions at the landscape level and serves as a useful tool in terms of forging sustainable land use strategies