Hidden Addressing Encoding for DNA Storage

DNA is a natural storage medium with the advantages of high storage density and long service life compared with traditional media. DNA storage can meet the current storage requirements for massive data. Owing to the limitations of the DNA storage technology, the data need to be converted into short DNA sequences for storage. However, in the process, a large amount of physical redundancy will be generated to index short DNA sequences. To reduce redundancy, this study proposes a DNA storage encoding scheme with hidden addressing. Using the improved fountain encoding scheme, the index replaces part of the data to realize hidden addresses, and then, a 10.1 MB file is encoded with the hidden addressing. First, the Dottup dot plot generator and the Jaccard similarity coefficient analyze the overall self-similarity of the encoding sequence index, and then the sequence fragments of GC content are used to verify the performance of this scheme. The final results show that the encoding scheme indexes with overall lower self-similarity, and the local thermodynamic properties of the sequence are better. The hidden addressing encoding scheme proposed can not only improve the utilization of bases but also ensure the correct rate of DNA storage during the sequencing and decoding processes

Influence of Aging Time on Vertical Static Stiffness of Air Spring

To study the aging mechanism of air springs, the effect of aging time on the vertical static stiffness of an air spring was systematically analyzed by means of an accelerated aging test and finite element simulation. Accelerated aging tests were carried out on the entire air spring, rubber material, and cord material, and the vertical static stiffness and elastic moduli of the rubber and cord materials of the entire air spring were obtained with aging time. The finite element simulation model of the air spring was established. Based on the experimental data, the influences of the elastic moduli of the rubber and cord materials, aged for different times, and the cord angle on the vertical static stiffness of an air spring were simulated and analyzed, and the law of the influence of aging on the vertical static stiffness characteristics of air springs was revealed

Influence of Aging Time on Vertical Static Stiffness of Air Spring

To study the aging mechanism of air springs, the effect of aging time on the vertical static stiffness of an air spring was systematically analyzed by means of an accelerated aging test and finite element simulation. Accelerated aging tests were carried out on the entire air spring, rubber material, and cord material, and the vertical static stiffness and elastic moduli of the rubber and cord materials of the entire air spring were obtained with aging time. The finite element simulation model of the air spring was established. Based on the experimental data, the influences of the elastic moduli of the rubber and cord materials, aged for different times, and the cord angle on the vertical static stiffness of an air spring were simulated and analyzed, and the law of the influence of aging on the vertical static stiffness characteristics of air springs was revealed

Study on the Weight Loss Effect and Mechanism of Turmeric Combined with Hawthorn on C57 Obese Mice

Objective: To evaluate the weight loss effect of turmeric combined with hawthorn and explore its weight loss mechanism. Methods: Thirty-two C57BL/6J mice were randomly divided into four groups: normal group, normal intervention group, high-fat group and high-fat intervention group, 8 mice in each group. The mice were gavaged with the corresponding drugs regularly every day, and the changes of adiposity, leanness, food intake and water consumption were detected regularly every week. The random blood glucose of the mice was measured at the same time every two weeks, after 12 weeks, the mice were removed from the cervical spine and the specimens were collected. The contents of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST) were measured in serum. The morphological changes of fat and liver tissue were observed by HE staining of epididymal fat and liver. The relative mRNA expressions of cholesterol 7α-hydroxylase (CYP7A1), fatty acid synthase (FAS), Liver X Receptorα (LXRα), Sterol Regulatory Element Binding Protein 2 (SREBP2), Sterol Regulatory Element Binding Protein (SREBP1C), Steary-coenzyme A dehydro-synthase-1 (SCD1) in liver were measured by real time polymerase chain reaction (RT-PCR). Results: Compared with the high-fat group, the body mass, fat percentage, TG, TC, LDL-C of mice in the high-fat intervention group were significantly decreased, lean meat percentage and HDL-C were significantly increased (P<0.05), and there was no significant difference in food intake and water intake. It could inhibit adipocyte hypertrophy and reduce fat deposition. And their liver cell vacuolation and inflammatory infiltration were improved. The mRNA expression level of CYP7A1 in liver was significantly increased (P<0.05). The mRNA expression levels of LXRα, SREBP1C, SCD and FAS were extremely significantly decreased (P<0.01). There was no significant change in the expression level of SREBP2 mRNA. Conclusion: Turmeric-hawthorn has good weight loss effects, and its weight loss mechanism is related to the regulation of mRNA expression levels in related genes

Measurement and Correlation of the Solubility of ε‑CL-20 in 12 Organic Solvents at Temperatures Ranging from 278.15 to 318.15 K

The solubility of ε-CL-20 was assessed in 12 organic solvents by the equilibrium method at temperatures ranging from 278.15 to 318.15 K and 0.1 MPa. The order of the solubility of ε-CL-20 was ethyl acetate > butyl acetate > triethyl-orthoformate > methanol > ethanol > propanol > 1-butanol > isopropyl alcohol > <i>m</i>-xylene > chloroform > toluene > cyclohexane. The modified Apelblat equation was used to correlate the experimental solubility values at these temperatures in these solvents and the results showed good agreement with the experimental values