METABOLİK SENDROM KLİNİK PARAMETRELERİNDE SR-BI GEN POLİMORFİZMLERİ ETKİSİNİN ARAŞTIRILMASI

AMAÇ: Metabolik Sendrom (MetS) dünya genelinde giderek yayılan ve çok sayıda insanı etkileyen önemli bir morbidite nedenidir. Yüksek trigliserid (TG) düzeyi ve düşük HDL düzeyi MetS teşhisindeki önemli faktörlerdir. SR-BI’ın ateroskleroz üzerindeki koruyucu etkisi ters kolesterol transportu sürecine katkıda bulunmasıyla gerçekleştirdiği (1) için rs 5888 ve rs 4238001 gen varyantlarının metabolik sendromlu hastaların klinik parametrelerine etkilerini araştırmayı hedefledik. MATERYAL VE METOD: 30-65 yaş aralığında 104 erkek metabolik sendromlu hasta ile metabolik sendrom hikayesi olmayan sağlıklı 100 erkek bireyin bulunduğu kontrol grubundan oluşmaktadır (SU TF Etik Kurul Karar No: 2015/19). Kan örneklerinden DNA izole edilip, saflık tayinleri ve miktarları hesaplandı. SR-BI genine ait ekzon 8’deki C>T (rs5888) değişimi ve ekzon 1’deki C>T (rs4238001) değişimi SNaPshot multipleks sistemi ile incelendi (Tablo 1, 2, 3 ve Şekil 1). Hasta ve kontrol gruplarında genotip ve allel dağılımları istatistiksel analizle (SPSS18) incelendi. BULGULAR: rs4238001 polimorfizminin hastalığa yatkınlık riskini artırdığı, rs5888 polimorfizminin ise etkisinin olmadığı görülmüştür. Hasta grubu rs4238001 genotiplere göre gruplandığında; kilo, vki, bel çevresi, HDL ve trigliserid ölçütlerinin arasında herhangi bir ilişki belirlenememiştir (pkilo=0,952; pVKİ=0,659; pbel çevresi= 0,303; pHDL= 0,622; ptrigliserid= 0,661) (Tablo 4). TARTIŞMA: Popülasyonumuzda rs4238001 ve HDL ya da diğer klinik veriler arasında bir ilişkiye rastlanmamıştır

Large-scale proteomic analysis of patients with type 2 diabetes mellitus and atherosclerosis using a label-free LC-MS/MS approach

Objective: Type 2 diabetes mellitus (T2D) is a metabolic disease whose molecular events have not yet been fully clarified. However, next-generation powerful molecular approaches such as mass spectrometry (MS)-based proteomics holds promise. In this study, we aimed to reveal the protein profile of serum samples obtained from patients with T2D and atherosclerotic cardiovascular disease using the high-resolution liquid chromatography (LC)-MS/MS system. Materials and Methods: Immune depletion was performed for the top 12 abundant proteins in 10 μl serum samples taken from individuals. Then, tryptic peptides were obtained from total proteins by applying a digestion protocol. Accordingly, reduction, alkylation, and digestion with trypsin enzyme were carried out, respectively. Tryptic peptides were analyzed in an ultra-high-pressure LC-MS/MS system with a label-free proteomic approach. The raw data were processed using the software program. Results: LC-MS/MS analyses revealed 120 proteins with significant expression changes. Some of these proteins were associated with inflammation, lipid transport, and oxidative stress, which are known to play an important role in T2D and its complications. Conclusion: As a result, LC-MS/MS analyses highlighted the proteins that will provide predictions in the treatment and course of T2D. We believe that validation of these proteins with targeted proteomic approaches in a larger sample in further studies will contribute to the development of clinically usable panels

Effects of Different Crystalloid Fluids on Renal Tissue in an Experimental Model of Hemorrhagic Shock

Objective:The type of fluid that should be used in uncontrollable hemorrhages remains an area of research. This study was designed to compare the effects of resuscitation with Ringer’s lactate (RL) solution versus a normal saline (NS) solution on hemodynamics, renal tissue histopathology, coagulation, and apoptosis in a rat model of hemorrhagic shock.Methods:The study employed groups designated as the control, hemorrhage, NS, and RL groups. Heart rate, mean arterial pressure, and respiratory rate were monitored. Annexin A5 values were assayed, rotational thromboelastometry analysis was performed, and excised kidney tissue samples were histopathologically analyzed.Results:Blood pressure levels were found to be significantly higher in the control group than those measured in the other groups. While the clotting time (CT) and clot formation time (CFT) in the hemorrhage group were significantly longer than those in the control and RL groups, the CT and CFT measured in the control group were significantly shorter compared to the RL group. The mean Annexin A5 level was in the hemorrhage group, which was significantly higher compared to the other groups. In the renal histopathological evaluation, the scores of proximal tubular injury, distal renal tubular injury, and interstitial renal tubular injury were found to be significantly lower in the control group compared to the other groups.Conclusion:This study demonstrated that NS or RL can be used safely to improve the hemodynamic symptoms resulting from hemorrhagic shock as a means to reduce apoptosis, and to decrease findings in favor of coagulopathy in bedside coagulation tests during the early stages of hemorrhagic shock until the time of starting a blood transfusion