New CagL amino acid polymorphism patterns of helicobacter pylori in peptic ulcer and non-ulcer dyspepsia

Background and Objectives: Helicobacter pylori infection is associated with chronic gastritis, ulcers, and gastric cancer. The H. pylori Type 4 secretion system (T4SS) translocates the CagA protein into host cells and plays an essential role in initiating gastric carcinogenesis. The CagL protein is a component of the T4SS. CagL amino acid polymorphisms are correlated with clinical outcomes. We aimed to study the association between CagL amino acid polymorphisms and peptic ulcer disease (PUD) and non-ulcer dyspepsia (NUD). Materials and Methods: A total of 99 patients (PUD, 46; NUD, 53) were enrolled and screened for H. pylori by qPCR from antrum biopsy samples. The amino acid polymorphisms of CagL were analyzed using DNA sequencing, followed by the MAFFT sequence alignment program to match the amino acid sequences. Results: Antrum biopsy samples from 70 out of 99 (70.7%) patients were found to be H. pylori DNA-positive. A positive band for cagL was detected in 42 out of 70 samples (PUD, 23; NUD, 19), and following this, these 42 samples were sequenced. In total, 27 different polymorphisms were determined. We determined three CagL amino acid polymorphism combinations, which were determined to be associated with PUD and NUD. Pattern 1 (K35/N122/V134/T175/R194/E210) was only detected in PUD patient samples and was related to a 1.35-fold risk (p = 0.02). Patterns 2 (V41/I134) and 3 (V41/K122/A171/I174) were found only in NUD patient samples and were linked to a 1.26-fold increased risk (p = 0.03). Conclusions: We observed three new patterns associated with PUD and NUD. Pattern 1 is related to PUD, and the other two patterns (Patterns 2 and 3) are related to NUD. The patterns that we identified include the remote polymorphisms of the CagL protein, which is a new approach. These patterns may help to understand the course of H. pylori infection.Istanbul Aydin University Scientific Research Projects Uni

Kolorektal kanserlerde HLA-G'nin doğal öldürücü İnhibitör Reseptörler ile etkileşiminin incelenmesi

Amaç: Malign tümörlerin gelişimi yalnızca neoplastik transformasyonun değil aynı zamanda anormal hücreleri elimine etmede konakçı direncinin başarısızlığının da bir sonucudur. Kolorektal kanserinde (KRK), HLA-G’nin hücre yüzeyinde artışı ve doğal öldürücü (NK) hücre işlevlerinde bozulması gibi bireyin immün sisteminde çok sayıda değişiklikler görülmektedir. Çalışmamızda, sHLA-G seviyelerinin belirlenmesi ve tümörlü dokuda HLA-G ifadesinin kayıp yada yokluğu ve NK hücrelerinin öldürücü inhibitör reseptör (KIR) yüzey belirteçlerinin tümör dokusuna infiltrasyonun incelenmesi amaçlanmıştır. Gereç-Yöntem: Bakırköy Dr. Sadi Konuk Eğitim Araştırma Hastanesi Genel Cerrahi Kliniği tarafından kolorektal kanseri tanısı konmuş ve opere edilmiş 36 hasta (24E/12K) 40 sağlıklı kontrol (22K/18E) çalışmaya dahil edildi. Hastaların yaş ortalaması 62,33±11,49 yıl (yaş aralığı: 33-78) iken kontrol grubunun 50,03±11,30 yıl (yaş aralığı: 22-71) olarak bulunmuştur. Hasta ve kontrollerde ELISA yöntemi ile sHLA-G seviyeleri ve tümörlü dokuda immünohistokimya (IHK) yöntemi aracılığıyla HLA-G, NK hücrelerinin öldürücü inhibitör reseptör (KIR) yüzey belirteçleri incelendi. Bulgular: IHK çalışmasının sonuçlarına göre hastaların %86,1’inde KIR pozitifliği, %16,7’sinde HLA-G pozitifliği gözlenmiştir. HLA-G pozitifliği hasta grubunda, kontrol grubuna oranla yüksek saptanmıştır (p=0.009). Sağlıklı kontrollerle kıyaslandığında KRK’li hasta dokularındaki KIR immün pozitifliğinde önemli ölçüde artış gözlenmiştir (p<0.0001). Tümör kesitlerinde, boyanma yoğunluğunun aynı tümör içindeki bir alandan diğerine değiştiği belirlenmiştir. Hasta ve kontrol serumlarındaki sHLA-G seviyeleri arasında bir farklılık gözlenmemiştir (p=0.871). Sonuç: KRK’li hastalardaki HLA-G ve KIR belirteçleri ifadesinin artış gösterdiği gözlenmiş ve dolayısıyla KRK’de güçlü, bağımsız ve faydalı bir prognostik belirteç olabileceği düşünülmüştür. Sonuçlarımız HLA-G ve KIR belirteçlerinin gelecekteki immünterapötik yaklaşımlar için umut vaad edici bir hedef olabileceğini düşündürmektedir

In vitro effects of rabeprazole on human pylorus tone

WOS: 000352750400009PubMed ID: 25843074Background/Aims It has been reported that proton pump inhibitors induce relaxation in different types of smooth muscles. The aim of this study is to investigate in vitro effects of proton pump inhibitors on human pylorus muscle. Methods Pyloric sphincters were studied in 10 patients who were operated for stomach cancer. In isolated organ bath, control and response to rabeprazole were recorded following contraction with carbachol. During the treatment experiment, while distilled water was applied during the control experiment in every 5 minutes, rabeprazole was administered in every 5 minutes at doses of 10(-6), 10(-6), 10(-4), and 10(-3) M respectively. Contraction frequencies, maximum contraction values and muscle tones were measured. Results The contraction frequencies in the control group were greater than the rabeprazole group in the second, third and fourth intervals while the maximum contraction values in the rabeprazole group were lower in the fourth interval. Even though muscles tones were not different in both groups during all intervals, it was remarkable that the muscle tone was significantly decreased in the rabeprazole group during the fourth interval compared to the first and second intervals. Conclusions In the present study, high doses of rabeprazole reduced contraction frequencies, maximum contraction values, and muscle tone of human pylorus.Yeditepe UniversityThe present study was supported by Yeditepe University. The authors are thankful to Ece Genc, Prof. Dr. for her invaluable mentorship

Increased interleukin 1 and tumor necrosis factor alpha levels after thyroid surgery

OBJECTIVE: The aim of this study was to consider levels of the proinflammatory cytokines IL-1 and TNFα after thyroid surgery. MATERIAL AND METHODS: A total of 200 patients who underwent total thyroidectomy enrolled in this study. Drain fluid samples were taken. IL-1 and TNFα results and their relationship with other factors were analyzed. RESULTS: There was a positive correlation between IL-1 and hyperthyroidism (r(s)=0.614, p<0.001), operative time (r(s)=0.770, p<0.001), and excised thyroid volume (r(s)=0.829, p<0.001). Moreover, there was a positive correlation between TNFα and hyperthyroidism (r(s)=0.430, p<0.001), operative time (r(s)=0.392, p<0.001), and excised thyroid volume (r(s)=0.398, p<0.001). CONCLUSION: The results of this study showed us that the parameters related to increased proinflammatory cytokine levels after thyroid surgery were hyperthyroidism, operative time, and excised thyroid volume, but this increase did not show us any clinical outcomes related to these parameters

Increased interleukin 1 and tumor necrosis factor alpha levels after thyroid surgery

OBJECTIVE: The aim of this study was to consider levels of the proinflammatory cytokines IL-1 and TNFα after thyroid surgery. MATERIAL AND METHODS: A total of 200 patients who underwent total thyroidectomy enrolled in this study. Drain fluid samples were taken. IL-1 and TNFα results and their relationship with other factors were analyzed. RESULTS: There was a positive correlation between IL-1 and hyperthyroidism (r(s)=0.614, p<0.001), operative time (r(s)=0.770, p<0.001), and excised thyroid volume (r(s)=0.829, p<0.001). Moreover, there was a positive correlation between TNFα and hyperthyroidism (r(s)=0.430, p<0.001), operative time (r(s)=0.392, p<0.001), and excised thyroid volume (r(s)=0.398, p<0.001). CONCLUSION: The results of this study showed us that the parameters related to increased proinflammatory cytokine levels after thyroid surgery were hyperthyroidism, operative time, and excised thyroid volume, but this increase did not show us any clinical outcomes related to these parameters