sj-docx-1-pac-10.1177_18344909241226761 - Supplemental material for Sleep spindles consolidate declarative memory with tags: A meta-analysis of adult data

Supplemental material, sj-docx-1-pac-10.1177_18344909241226761 for Sleep spindles consolidate declarative memory with tags: A meta-analysis of adult data by Peiyao Chen, Chao Hao and Ning Ma in Journal of Pacific Rim Psychology</p

sj-docx-2-pac-10.1177_18344909241226761 - Supplemental material for Sleep spindles consolidate declarative memory with tags: A meta-analysis of adult data

Supplemental material, sj-docx-2-pac-10.1177_18344909241226761 for Sleep spindles consolidate declarative memory with tags: A meta-analysis of adult data by Peiyao Chen, Chao Hao and Ning Ma in Journal of Pacific Rim Psychology</p

Cell Environment-Differentiated Self-Assembly of Nanofibers

Employing cellular environment for the self-assembly of supramolecular nanofibers for biological applications has been widely explored. But using one precursor to differentiate the extra- and intracellular environments to self-assemble into two different nanofibers remains challenging. With the knowledge that the extracellualr environment of some cancer cells contains large amounts of alkaline phosphatase (ALP) while their intracellular environment is glutathione (GSH)-abundant in mind, we rationally designed a precursor Cys­(SEt)-Glu-Tyr­(H₂PO₃)-Phe-Phe-Gly-CBT (<b>1</b>) that can efficiently yield amphiphilic <b>2</b> and <b>2-D</b> to self-assemble into two different nanofibers in hydrogels under the sequential treatment of ALP and GSH. We envision that, by employing a click condensation reaction, this work offers a platform for facilely postmodulation of supramolecular nanofibers, and the versatile precursor <b>1</b> could be used to kill two birds with one stone

Using “On/Off” ¹⁹F NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates

Tyrosine kinase and phosphatase are two important, antagonistic enzymes in organisms. Development of noninvasive approach for sensing their activity with high spatial and temporal resolution remains challenging. Herein, we rationally designed a hydrogelator Nap-Phe-Phe­(CF₃)-Glu-Tyr-Ile-OH (<b>1a</b>) whose supramolecular hydrogel (i.e., Gel <b>1a</b>) can be subjected to tyrosine kinase-directed disassembly, and its phosphate precursor Nap-Phe-Phe­(CF₃)-Glu-Tyr­(H₂PO₃)-Ile-OH (<b>1b</b>), which can be subjected to alkaline phosphatase (ALP)-instructed self-assembly to form supramolecular hydrogel Gel <b>1b</b>, respectively. Mechanic properties and internal fibrous networks of the hydrogels were characterized with rheology and cryo transmission electron microscopy (cryo-TEM). Disassembly/self-assembly of their corresponding supramolecular hydrogels conferring respective “On/Off” ¹⁹F NMR/MRI signals were employed to sense the activity of these two important enzymes <i>in vitro</i> and in cell lysates for the first time. We anticipate that our new ¹⁹F NMR/magnetic resonance imaging (MRI) method would facilitate pharmaceutical researchers to screen new inhibitors for these two enzymes without steric hindrance

Nanocomputed Tomography Imaging of Bacterial Alkaline Phosphatase Activity with an Iodinated Hydrogelator

Alkaline phosphatase (ALP) is an important enzyme, but direct imaging of ALP activity with high spatiotemporal resolution remains challenging. In this work, we rationally designed an iodinated hydrogelator precursor Nap-Phe-Phe­(I)-Tyr­(H₂PO₃)-OH (<b>1P</b>) which self-assembles into nanofibers to form hydrogel under the catalysis of ALP. With this property of concentrating iodine atoms at the locations of ALP, <b>1P</b> was successfully applied for direct nanocomputed tomography (nano-CT) imaging of ALP activity in bacteria for the first time. We envision that, on the basis of this pioneering work, new hydrogelators containing more iodine atoms (e.g., five iodine atoms in <b>1P</b>) will be designed for better nano-CT imaging of ALP activity with higher CT contrast in the near future

Nanocomputed Tomography Imaging of Bacterial Alkaline Phosphatase Activity with an Iodinated Hydrogelator

Alkaline phosphatase (ALP) is an important enzyme, but direct imaging of ALP activity with high spatiotemporal resolution remains challenging. In this work, we rationally designed an iodinated hydrogelator precursor Nap-Phe-Phe­(I)-Tyr­(H₂PO₃)-OH (<b>1P</b>) which self-assembles into nanofibers to form hydrogel under the catalysis of ALP. With this property of concentrating iodine atoms at the locations of ALP, <b>1P</b> was successfully applied for direct nanocomputed tomography (nano-CT) imaging of ALP activity in bacteria for the first time. We envision that, on the basis of this pioneering work, new hydrogelators containing more iodine atoms (e.g., five iodine atoms in <b>1P</b>) will be designed for better nano-CT imaging of ALP activity with higher CT contrast in the near future