4 research outputs found
Quantitative Statistical Analysis of Standard and Human Blood Proteins from Liquid Chromatography, Electrospray Ionization, and Tandem Mass Spectrometry
It will be important to determine if the parent and fragment
ion
intensity results of liquid chromatography, electrospray ionization
and tandem mass spectrometry (LC–ESI–MS/MS) experiments
have been randomly and independently sampled from a normal population
for the purpose of statistical analysis by general linear models and
ANOVA. The tryptic parent peptide and fragment ion <i>m</i>/<i>z</i> and intensity data in the mascot generic files
from LC–ESI–MS/MS of purified standard proteins, and
human blood protein fractionated by partition chromatography, were
parsed into a Structured Query Language (SQL) database and were matched
with protein and peptide sequences provided by the X!TANDEM algorithm.
The many parent and/or fragment ion intensity values were log transformed,
tested for normality, and analyzed using the generic Statistical Analysis
System (SAS). Transformation of both parent and fragment intensity
values by logarithmic functions yielded intensity distributions that
closely approximate the log-normal distribution. ANOVA models of the
transformed parent and fragment intensity values showed significant
effects of treatments, proteins, and peptides, as well as parent versus
fragment ion types, with a low probability of false positive results.
Transformed parent and fragment intensity values were compared over
all sample treatments, proteins or peptides by the Tukey-Kramer Honestly
Significant Difference (HSD) test. The approach provided a complete
and quantitative statistical analysis of LC–ESI–MS/MS
data from human blood
Quantitative Statistical Analysis of Standard and Human Blood Proteins from Liquid Chromatography, Electrospray Ionization, and Tandem Mass Spectrometry
It will be important to determine if the parent and fragment
ion
intensity results of liquid chromatography, electrospray ionization
and tandem mass spectrometry (LC–ESI–MS/MS) experiments
have been randomly and independently sampled from a normal population
for the purpose of statistical analysis by general linear models and
ANOVA. The tryptic parent peptide and fragment ion <i>m</i>/<i>z</i> and intensity data in the mascot generic files
from LC–ESI–MS/MS of purified standard proteins, and
human blood protein fractionated by partition chromatography, were
parsed into a Structured Query Language (SQL) database and were matched
with protein and peptide sequences provided by the X!TANDEM algorithm.
The many parent and/or fragment ion intensity values were log transformed,
tested for normality, and analyzed using the generic Statistical Analysis
System (SAS). Transformation of both parent and fragment intensity
values by logarithmic functions yielded intensity distributions that
closely approximate the log-normal distribution. ANOVA models of the
transformed parent and fragment intensity values showed significant
effects of treatments, proteins, and peptides, as well as parent versus
fragment ion types, with a low probability of false positive results.
Transformed parent and fragment intensity values were compared over
all sample treatments, proteins or peptides by the Tukey-Kramer Honestly
Significant Difference (HSD) test. The approach provided a complete
and quantitative statistical analysis of LC–ESI–MS/MS
data from human blood
Quantitative Statistical Analysis of Standard and Human Blood Proteins from Liquid Chromatography, Electrospray Ionization, and Tandem Mass Spectrometry
It will be important to determine if the parent and fragment
ion
intensity results of liquid chromatography, electrospray ionization
and tandem mass spectrometry (LC–ESI–MS/MS) experiments
have been randomly and independently sampled from a normal population
for the purpose of statistical analysis by general linear models and
ANOVA. The tryptic parent peptide and fragment ion <i>m</i>/<i>z</i> and intensity data in the mascot generic files
from LC–ESI–MS/MS of purified standard proteins, and
human blood protein fractionated by partition chromatography, were
parsed into a Structured Query Language (SQL) database and were matched
with protein and peptide sequences provided by the X!TANDEM algorithm.
The many parent and/or fragment ion intensity values were log transformed,
tested for normality, and analyzed using the generic Statistical Analysis
System (SAS). Transformation of both parent and fragment intensity
values by logarithmic functions yielded intensity distributions that
closely approximate the log-normal distribution. ANOVA models of the
transformed parent and fragment intensity values showed significant
effects of treatments, proteins, and peptides, as well as parent versus
fragment ion types, with a low probability of false positive results.
Transformed parent and fragment intensity values were compared over
all sample treatments, proteins or peptides by the Tukey-Kramer Honestly
Significant Difference (HSD) test. The approach provided a complete
and quantitative statistical analysis of LC–ESI–MS/MS
data from human blood
Quantitative Statistical Analysis of Standard and Human Blood Proteins from Liquid Chromatography, Electrospray Ionization, and Tandem Mass Spectrometry
It will be important to determine if the parent and fragment
ion
intensity results of liquid chromatography, electrospray ionization
and tandem mass spectrometry (LC–ESI–MS/MS) experiments
have been randomly and independently sampled from a normal population
for the purpose of statistical analysis by general linear models and
ANOVA. The tryptic parent peptide and fragment ion <i>m</i>/<i>z</i> and intensity data in the mascot generic files
from LC–ESI–MS/MS of purified standard proteins, and
human blood protein fractionated by partition chromatography, were
parsed into a Structured Query Language (SQL) database and were matched
with protein and peptide sequences provided by the X!TANDEM algorithm.
The many parent and/or fragment ion intensity values were log transformed,
tested for normality, and analyzed using the generic Statistical Analysis
System (SAS). Transformation of both parent and fragment intensity
values by logarithmic functions yielded intensity distributions that
closely approximate the log-normal distribution. ANOVA models of the
transformed parent and fragment intensity values showed significant
effects of treatments, proteins, and peptides, as well as parent versus
fragment ion types, with a low probability of false positive results.
Transformed parent and fragment intensity values were compared over
all sample treatments, proteins or peptides by the Tukey-Kramer Honestly
Significant Difference (HSD) test. The approach provided a complete
and quantitative statistical analysis of LC–ESI–MS/MS
data from human blood