Data_Sheet_1_Comparative assessment of Cucurbita moschata seed polypeptides toward the protection of human skin cells against oxidative stress-induced aging.docx

Skin aging has attracted much attention among the current aging population of society. The seeds of Cucurbita moschata possess a variety of potential biological activities as a healthy diet. However, limited information is available on the skin-antiaging properties of C. moschata seed protein and its hydrolysate. Herein, we developed a novel strategy for protecting human skin cells against oxidative stress-induced aging by C. moschata seed polypeptides. C. moschata seed polypeptides (CSPs) with different molecular weight distributions were successfully prepared by controlling the protease hydrolysis time. The proportions of 2O2-induced aging of HSF cells. To explain this phenomenon, cell lifespan, intracellular ROS level, superoxide dismutase (SOD) activity, and glutathione (GSH) content were investigated to reveal the interactions between P-3 and antiaging. With the increase in P-3 concentration, the ROS level significantly decreased, and the SOD activity and GSH content significantly increased in H2O2-induced HSF cells. These findings indicated that CSPs have the potential to inhibit skin aging, which could be advantageous in the health industry for providing personal care.</p

ICMJE Disclosures

<p>Supplemental material, FAI753160-ICMJE for Strain Distribution in the Anterior Inferior Tibiofibular Ligament, Posterior Inferior Tibiofibular Ligament, and Interosseous Membrane Using Digital Image Correlation by Daorong Xu, Yibei Wang, Chunyu Jiang, Maoqing Fu, Shiqi Li, Lei Qian, Peidong Sun and Jun Ouyang in Foot & Ankle International</p

Linear parameters and angular measurements for vertebral characterization (Mean±SD).

<p> Compared with l/rTPA, l/rSPA, l/rDSIP, l/rDTIP between males and females in C3, P<0.05;</p><p> Statistically significant differences in males of l/rTPA (C3, C4), and l/rDSIP (C5), P<0.05,</p><p>^& Statistically significant differences in females of l/rTPA(C7) and l/rDSIP (C7) P<0.05.</p

Linear parameters measured of the aVBH, mVBD and mVBW (Mean± SD, mm).

<p>Linear parameters measured of the aVBH, mVBD and mVBW (Mean± SD, mm).</p

The process of part of measuring parameters using Mimics 14.11 software.

<p>A: The target vertebra was chosen in Mimics 14.11 software. Three lines (AP-UP, ME-TE, PP-UP) were drawn and the cutting plane was adopted with the midpoint of the three lines. 1, AP-UP: The line between left and right anterior part of unicinate process. 2, ME-TF: The line between left and right medial edge of transverse foramen. 3, PP-UP: The line between left and right posterior part of unicinate process. B: Profile of the cutting plane can be observed by rotation. C: The cutting vertebral body was colored. D: The aVBH was measured.</p

Linear parameters for pedicle characterization (Mean±SD, mm).

<p>^# Compared with rPAL between males and females in C6; P>0.05,</p><p> Compared with l/rOPW and l/rPAL between males and females in C7, P>0.05,</p><p> Statistically significant differences in males of l/rPAL (C7); P<0.05,</p><p>^& Statistically significant differences in females of l/rPAL (C7) P<0.05.</p