Polymerase chain reaction detection of rabbit DNA in food and animal feed

[EN] A polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was developed for the specific identification of rabbit DNA (Oryctolagus cuniculus) in food and feedstuffs. The specificity of the primers was verified by PCR analysis of DNA from 32 non-target species including mammals, birds, fish, and plant species. Analysis of experimental mixtures demonstrated the presence of rabbit-derived materials in the range of 0.1-100%. Prolonged heat treatment (up to 133ºC for 20 min at 300 kPa) applied to rabbit muscle/oats binary mixtures did not affect the performance of the method, which could therefore be said to be very useful for the accurate identification of rabbit materials in products submitted to denaturing technologies when other methods are not suitable.This study was supported by Grant Nº. AGL 2007-60077 from the Spanish Ministerio de Educación y Ciencia and the Programa de Vigilancia Sanitaria S-0505/AGR/000265 of the Comunidad de Madrid (Spain). Irene Martín is the recipient of a fellowship from the Universidad Complutense de Madrid (Spain). Violeta Fajardo and María Rojas are recipients of fellowships from the Ministerio de Educación y Ciencia (Spain).Martín, I.; García, T.; Fajardo, V.; Rojas, M.; Pegels, N.; Hernández, P.; González, I.... (2009). Polymerase chain reaction detection of rabbit DNA in food and animal feed. World Rabbit Science. 17(1):27-34. doi:10.4995/wrs.2009.667273417

Comparing nuclear power trajectories in Germany and the UK: from ‘regimes' to ‘democracies’ in sociotechnical transitions and Discontinuities

This paper focuses on arguably the single most striking contrast in contemporary major energy politics in Europe (and even the developed world as a whole): the starkly differing civil nuclear policies of Germany and the UK. Germany is seeking entirely to phase out nuclear power by 2022. Yet the UK advocates a ‘nuclear renaissance’, promoting the most ambitious new nuclear construction programme in Western Europe.Here,this paper poses a simple yet quite fundamental question: what are the particular divergent conditions most strongly implicated in the contrasting developments in these two countries. With nuclear playing such an iconic role in historical discussions over technological continuity and transformation, answering this may assist in wider understandings of sociotechnical incumbency and discontinuity in the burgeoning field of‘sustainability transitions’. To this end, an ‘abductive’ approach is taken: deploying nine potentially relevant criteria for understanding the different directions pursued in Germany and the UK. Together constituted by 30 parameters spanning literatures related to socio-technical regimes in general as well as nuclear technology in particular, the criteria are divided into those that are ‘internal’ and ‘external’ to the ‘focal regime configuration’ of nuclear power and associated ‘challenger technologies’ like renewables. It is ‘internal’ criteria that are emphasised in conventional sociotechnical regime theory, with ‘external’ criteria relatively less well explored. Asking under each criterion whether attempted discontinuation of nuclear power would be more likely in Germany or the UK, a clear picture emerges. ‘Internal’ criteria suggest attempted nuclear discontinuation should be more likely in the UK than in Germany– the reverse of what is occurring. ‘External’ criteria are more aligned with observed dynamics –especially those relating to military nuclear commitments and broader ‘qualities of democracy’. Despite many differences of framing concerning exactly what constitutes ‘democracy’, a rich political science literature on this point is unanimous in characterising Germany more positively than the UK. Although based only on a single case,a potentially important question is nonetheless raised as to whether sociotechnical regime theory might usefully give greater attention to the general importance of various aspects of democracy in constituting conditions for significant technological discontinuities and transformations. If so, the policy implications are significant. A number of important areas are identified for future research, including the roles of diverse understandings and specific aspects of democracy and the particular relevance of military nuclear commitments– whose under-discussion in civil nuclear policy literatures raises its own questions of democratic accountability

Exploring the role of instrument design and instrument interaction for eco-innovation: a survey-based analysis of renewable energy innovation in Germany

Empirical research on eco-innovation has produced a substantive body of literature on the relevance of regulation for stimulating such innovation. Much of this work on the role of policy for eco-innovation relies on econometric analyses of company survey data. In this regard, the eco-innovation module introduced in 2008/9 in the Community Innova-tion Survey serves as an important data source that has helped improve our under-standing of the role of environmental and innovation policy for eco-innovation in the Eu-ropean Union (EU). However, so far, this data source has provided only limited oppor-tunities to generate insights into the role of instrument design and instrument interaction for eco-innovation. In this chapter, we present a first attempt to measure such aspects in a company innovation survey based on the example of renewable energy innovation in Germany. In particular, we explore to what extent the design of the German Renewa-ble Energy Sources Act (and the interaction of its feed-in tariffs with the EU emissions trading system) correlates with innovation in renewable power generation technologies. We find instrument design features but not instrument type to be related to eco-innovation. In addition, our exploratory study provides evidence for an interaction effect between climate policy and renewables support policy. Based on these findings, we discuss implications for future research on the role of policy in eco-innovation

Splenic B cell lymphoma with lymphocyte clusters in peripheral blood smears

EDTA induced clumping of lymphoid cells, both benign and malignant, in peripheral blood samples has been reported only rarely. Such clustering presents the laboratory and pathologist with unique difficulties in the accurate diagnosis of these disorders. A case of low grade B cell splenic lymphoma is presented with lymphocyte clumping in smears made from EDTA anticoagulated peripheral blood, the fourth case described in which neoplastic lymphoid cells form clusters in vitro in peripheral blood. The association with splenic lymphomas (three of the four cases) is intriguing but its significance remains uncertain. Key Words: EDTA • leucoagglutination • lymphocytes • lymphom