The use of cephalothin and triphenyltetrazolium chloride impregnated filter paper strips in the identification of Campylobacter species

Filter paper impregnated strips using cephalothin at 30 and 60 µg/ml and triphenyltetrazolium chloride at 20 mg/ml were prepared and used in the typing of catalase-positive Campylobacter species. There was no difference in the sensitivity of campylobacters to cephalothin at 30 µg/ml and 60 µg/ml. Results were as reported by other workers except for a C. jejuni strain which was resistant to the triphenyltetrazolium. The technique is nevertheless inexpensive and the results are consistent and easy to interpret.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

The use of sensitivity discs in the identification of Campylobacter species

Filter paper discs were impregnated with a solution containing 20 mg of triphenyltetrazolium chloride per millilitre, and used in the typing of catalase-positive Campylobacter species. Also used were filter paper discs impregnated with cephalothin at 30µg/ml, 60µg/ml and 3 mg/ml and nalidixic acid at the same concentrations, as well as commercially available discs containing 30µg of, respectively, cephalothin and nalidixic acid. Results obtained proved the technique to be reliable and easier to interpret than previously used methods, and laboratory prepared filter paper discs compared favourably with commercial discs.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

The characteristics of a variant strain of Brucella melitensis Rev I

Circumstantial evidence is presented for the occurrence of a variant of a vaccine strain of B. melitensis Rev 1, designated "FSA"(foreign South African). FSA resembles Rev 1 in its reactions to penicillin and streptomycin but reacts closer to a field strain of B. melitensis as regards dye (thionine and basic fuchsin) sensitivity and colony size. Although colonies of Rev 1 were consistently smaller than other B. melitensis strains, their size was 0,75 mm as opposed to the 1-2 mm reported in the literature, while B. melitensis 16M colonies were 1,25-1,5 mm as opposed to the 3-4 mm previously reported. Rev 1 was found to be urease positive, unless a test of low sensitivity was applied.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

The isolation and serology of the "FSA" Brucella melitensis Rev.1 mutant in a flock of sheep

A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of this infection in the field. An attempt was also made to determine whether vertical transmission occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the udder, supramammary lymphnodes and uterus. No significant relationship could be shown between the complement fixation test and bacterial isolation. The absence of any relationship between serological and bacteriological results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either serological reactions or were bacteriologically positive, thus no vertical transmission could be shown.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

The isolation and serology of the "FSA" Brucella melitensis Rev. 1 mutant in a flock of sheep

A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was\ud examined serologically and bacteriologically to detennine whether any relationship existed which would help in\ud the control of this infection in the field. An attempt was also made to determine whether vertical transmission\ud occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the\ud udder, supramammary lymphnodes and uterus. No significant relationship could be shown between the complement\ud fixation test and bacterial isolation. The absence of any relationship between serological and bacteriological\ud results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either\ud serological reactions or were bacteriologically positive, thus no vertical transmission could be shown