A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was\ud
examined serologically and bacteriologically to detennine whether any relationship existed which would help in\ud
the control of this infection in the field. An attempt was also made to determine whether vertical transmission\ud
occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the\ud
udder, supramammary lymphnodes and uterus. No significant relationship could be shown between the complement\ud
fixation test and bacterial isolation. The absence of any relationship between serological and bacteriological\ud
results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either\ud
serological reactions or were bacteriologically positive, thus no vertical transmission could be shown

Gummow, B.

Herr, S.

Pefanis, S.M.

Pieterson, P.M.

Venter, Catharina G.

Williamson, Catherine C.

A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was

examined serologically and bacteriologically to detennine whether any relationship existed which would help in

the control of this infection in the field. An attempt was also made to determine whether vertical transmission

occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the

udder, supramammary lymphnodes and uterus. No significant relationship could be shown between the complement

fixation test and bacterial isolation. The absence of any relationship between serological and bacteriological

results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either

serological reactions or were bacteriologically positive, thus no vertical transmission could be shown

ResearchOnline at James Cook University

The isolation and serology of the "FSA" Brucella melitensis Rev. 1 mutant in a flock of sheep

The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. 
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. 1, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of this infection in the field. An attempt was also made to determine whether vertical transmission occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the udder, supramammary lymphnodes and uterus. No significant relationship could be shown between the complement fixation test and bacterial isolation. The absence of any relationship between serological and bacteriological results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either serological reactions or were bacteriologically positive, thus no vertical transmission could be shown.lmchunu2014mn201

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Onderstepoort J. vet. Res., 55, 181-183 (1988) THE ISOLATION AND SEROLOGY OF THE "FSA" BRUCELLA MELITENSIS REV. 1 MUTANT IN A FLOCK OF SHEEP S.M. PEFANIS, B. GUMMOW, P.M. PIETERSON, CATHERINE C. WILLIAMSON, CATHARINA G. VENTER and S. HERR, Veterinary Research Institute, Onderstepoort 0 11 0 ABSTRACT PEFANIS, S. M., GUMMOW, B., PIETERSON, P. M., WILLIAMSON, CATHERINE C., VENTER, CATHARINA G. & HERR, S., 1988. The isolation and serology of the "FSA" Brucella melitensis Rev l mutant in a flock of sheep. Onderstepoort Jour!Ull ofVeterinary Research, 55, 181-183 ( 1988) A flock of sheep, known to be infected with the "FSA" mutant of Brucella melitensis Rev. I, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of this infection in the field. An attempt was also made to determine whether vertical transmission occurred. Twenty-one out of 62 sheep were bacteriologically positive. The best organs for isolation were the udder, supra mammary lymphnodes and uterus. No significant relationship could be shown between the comple-ment fixation test and bacterial isolation. The absence of any relationship between serological and bacterio-logical results agrees with a short-lived infection. None of the 24 lambs sacrificed at 5 months showed either serological reactions or were bacteriologically positive, thus no vertical transmission could be shown. INTRODUCTION The Rev. I strain of Brucella melitensis was first de-scribed. in 1955 (Herzberg & Elberg), and its biological properties by Elberg & Meyer in 1958 as cited by Alton & Elberg (1967). The use of this vaccine in sheep was first reporte~ by Renoux in 1958 (Alton & Elberg, 19?7), an~ It _was used for the prevention of Brucella ovzs mfection m ewes and rams in South Africa for the first time in 1960 (Van Drimmelen). The Rev. I vaccine is still currently in use in the Republic of South Africa to control B. avis inf~cti<;m i~ rams. The immunity resulting from Rev. ~ vaccma!Ion IS long-lasting and persistence of ~ev. _I. m sheep IS less than 6 months although this vanes With the age, pregnancy status at vaccination and the br~ed (Alton, _I 98?a). Ac~o~ding to Alton (19S5a), followmg the vaccmat10n of v1rgm kids and Iambs it has never been possible to recover Rev. I from the udder or the vagina afte~ p~rturition. The vaccination of pregnant s.heep or goats IS hkely to cause abortion with the excre-tiOn of Rev. 1, although this excretion does not last as long as that which follows an abortion with a field strain of B. melitensis (Alton, 1985a). Neeman, cited by Alton (1985a) was unable to produce changes in the virulence of Rev. I by passage through pregnant sheep. He also demonstrated that sheep excreting Rev. I at abortion were unable _to transmit infections to susceptible preg-nant s~eep m clos~ co~tact. According to Bosseray ( 1985) It appears as If strams of Rev. I in use in different part.s . of the world, differ considerably in immuno-gemcity but no lateral or vertical spread of Rev. I has been reported. The first isolate of the "FSA" mutant form of B melitensis. Rev. I was received at this laboratory fro~ Natal dunng March 1984. Subsequent to this, isolates from. sheep, goats, 2 humans and 2 batches of "Rev. 1" vaccme w7re received from the Cape Province and Transvaal m 1985 and 1986. The characteristics of this variant strain of Rev. I are described by Pieterson Gum-mow, Pefanis, Venter&Herr(1988). The "FSA'~ strain of B ~ melitensis resembles Rev. I in its reactions to penicillin and streptomycin but reacts closer to a field strain o~ B. n:elitensis as regards dye sensitivity and colony siZe (P1eterson et al., 1988). The current investi-gati?n d~als with the serological and bacteriological findmgs m a flock of sheep in which abortions had occurred and this variant strain of Rev. I had been Received 30 May 1988-Editor 181 isolated from the abortion material. The flock of sheep was acquired with a view to attempting to correlate the serological results with isolation successes in order to simplify control of this infection in the field. Whether vertical transmission to the progeny occurred was a fur-ther question which required elucidation. MATERIALS AND METHODS Animals The flock of sheep, with no history of any Brucella vaccination, consisted of 60 ewes, 2 rams and 24 suck-ling lams. Ten of the 60 ewes were still pregnant. Eleven of the ewes had already aborted at the time the flock was purchased. Serum was collected twice, with a 5 day interval, from all the sheep just prior to slaughter. The adults were euthanased with intravenous sodium pento-barbitone• and a post mortem examination was per-formed on each animal . Samples of lung, liver, spleen, left and right udder, left and right supramammary lymphnodes, iliac lymphnodes, internal inguinal lymph-nodes, ovaries or testes, uterus or epididymis, brain, heart and muscle from the pillars of the diaphragm were collected aseptically. Organs were also collected from the foetuses removed from the pregnant ewes. Samples collected from foetuses were: lung, liver, spleen, kid-ney, stomach contents, abomasum, rumen, brain and placenta. Serum was also collected from the 24 Iambs at 5 months of age. These lambs were then slaughtered and the same set of organs as for the adult sheep were col-lected. Bacterial isolation and culture media A bacteriological loop was used to plate out material taken from the freshly-cut surface of each organ. Five different media were used throughout the study, as described by Herr & Roux (1981), and each specimen was plated out on all 5 media. Plates were incubated at 37 oc in air and air plus 10 % C02• Plates were examined macroscopically and microscopically for the presence of typical Brucella colonies after 48, 96 and 114 h. Typing of isolates All the isolates were typed as described by Corbel & Hendry (1983), except for a few modifications as described by Pieterson et al. (1988). 1 Eutha-naze, Centaur Labs (Pty) Ltd, 36 Durban St., Johannesburg 2001 ISOLATION ANDSEROLOGYOFTHE "FSA" BRUCEUAMEUTENSISREV.i MUTANT TABLE I Organs from which B. melitensis Rev. I FSA were isolated • Animal No. ...; i I ...; ...: ... c:: ... ... 00 ~ -8 :8 c:: .. 3 .:: 0. -o ...J ~ ~ "' <f) <f) I + + + 6 + + + 7 12 + + 14 + + 21 + + + + 22 23 26 + 27 28 + + + 34 41 49 + 51 + + + + 53 + + + + 54 55 + 57 + 58 + + 59 + Total 3 5 5 6 7 Serology The complement fixation test (CFf) technique was as used for bovine brucellosis at the Veterinary Research Institute, Onderstepoort. The method was similar to that used by the Central Veterinary Laboratory, Weybridge (Morgan, Mackinnon, Gill, Gower & Norris, 1978), but adapted to microtitration by Herr, Bishop, Bolton & Van der Merwe (1979). The results are reported in Interna-tional Units per millilitre (IU/mf) (Herr, Williamson, Prigge & VanWyk, 1986). REsULTS 6 All the isolates were typed as the mutant "FSA" strain of B. melitensis Rev. 1 as described by Pieterson et al. (1988). Nineteen of the 62 sheep were positive bacte-riologically for Brucella. A further 2 sheep were classi-fied as positive for Brucella after the organism was isolated from the foetuses of these 2 ewes. Only 2 out of the 10 foetuses examined were positive for "FSA" on culture. None of the 24 lambs at 5 months showed any serological reaction or proved positive on isolation. Table 1 shows from which organs Brucella was isolated in each of the 21 sheep. Table 2 shows the relationship between the CFT and the isolation of Brucella from the adult sheep. A titre of less than 60 IU/mf was designated a low titre, 60--196 IU/mf a medium titre and more than 196 IU/mf a high titre. Ten of the sheep from which Brucella was isolated had titres of less than 60 IU/mf in the CFT test. The dams of the 2 positive foetuses were bacterio-logically negative, although the dams showed CFT titres 182 ...: I .... + ...... ~ -; "' "' .!l "' .9 -~ ~ -~ ~ ~ B ~ 6'o ~ ~ "' > ..9 0 ~ co <f) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 9 4 2 4 9 2 3 3 2 TABLE 2 Relationship between CFT titre and results of bacterial iiD-lation Serology Bacterial isolation CFTtitre (IU/mt') Positive Negative 196 10 13 60-196 I 5 60 10 23 Total 21 41 TABLE 3 The relationship between rising, falling and consistent CFT titres and bacterial isolation Serology Bacterial isolation CFT titres Positive Negative 2-4 x rising titre 8 8 2-4 X falling titre 2 13 Consistent titre ll 20 Total 21 41 of 480 and 784 IU/mf respectively. Both the rams had CFT titres of less than 60 IU/mf and both were negative on bacterial isolation. Table 3 shows the relationship between a changing CFT titre and bacterial isolation. A rising titre or con-sistent titre should theoretically indicate an active ?n~oing i!ifection whereas a falling titre could possibly mdicate pnor exposure. S.M. PEFANIS,B. GUMMOW,P. M. PIETERSON,C.C. WILLIAMSON, C. G. VENTER&S. HERR No further isolations of FSA have been reported from the field to date (January 1988). DISCUSSION Although no history of inoculation with any Brucella vaccine was reported the authors cannot guarantee that vaccination did not occur. However, if Brucella vaccina-tion had not occurred then the "FSA" mutant strain is capable of being laterally transmitted. The fact that none of the lambs, slaughtered at 5-6 months old, showed any serological reaction or were positive on bacteriological tests, would indicate that there was no vertical transmission of this mutant form of B. melitensis Rev. 1 (FSA). The most common organs from which isolates were made were the udder, supramammary lymphnodes and uterus which is in accordance with other authors (Alton, 1985b; Herr & Roux, 1981). This could be of zoonotic importance in the abattoir industry as the strain has already shown itself to be pathogenic in man (Pieterson et al., 1988). The isolation of B. melitensis from the ovaries in 4 out of 19 of the sheep was noteworthy as was the isolation from the heart muscle in 3 out of 19 cases, because these organs are not ~enerally referred to in the literature as suitable for isolation of Brucella. The isola-tion of Brucella from the lung tissue only in animal number 59 may be indicative of a very early infection and point to inhalation infection. Serological testing does not af~ar to be a very reliable method of indicating "FSA infected animals in the flock. The specificity of the CFf test calculated according to Cutler (1979) was 56% and the sensitivity was 52 %. The predictive value of the CFI' was calcu-lated at 58 %. The aim of Table 3 was to examine the possibility that a rising or consistent titre would indicate active ongoing infection whereas a falling titre could indicate prior exposure to Brucella with subsequent elimination of the infection. The difference between twofold rise and twofold decrease in titre as an indicator of infection is also of no significance as the results were equivocal. The absence of any significant relationship between serological and bacteriological results is diffi-cult to explain except in terms of a short-lived bacterial infection which is reported for Rev. 1 (Alton, 1985a). This also agrees with the apparent failure of the '' FSA'' strain to maintain infection in the field, as witnessed by the absence of further recoveries of '' FSA'' to date (Jan-uary 1988). 183 ACKNOWLEDGEMENTS The assistance of Dr J. G. Myburgh, Miss R. Knoetze and Mrs S. Marais is gratefully acknowledged. REFERENCES ALTON, G. G. & ELBERG, S. S., 1967. Rev. 1 Brucella melitensis vaccine. A review of ten years of study. Veterinary Bulletin, 37, 793--800. ALTON, G. G., 1985a. Rev. I and H38 Brucella melitensis vaccines. In: VERGER, J. M. & PLoMMET, M. (ed.). Brucella melitensis. Dordrecbt, Boston & Lancaster: Martinus Nijboff Publishers. ALTON, G. G., 1985b. The epidemiology of Brucella melitensis infec-tion in sheep and goats. In: YERGER, J. M. & PLoMMET, M. (ed.). Brucella melitensis. Dordrecbt, Boston & Lancaster: Martinus Nij-boff Publishers. BossERA Y, N., 1985. Quality control of four Rev. 1 antibrucella vac-cines. In: VERGER, J. M. & PLoMMET, M. (ed.). Brucella meliten-sis. Dordrecbt, Boston & Lancaster: Martinus NijboffPublishers. CORBEL, M. J. & HENDRY, D. McL.F.D., 1983. Methods for the identification of Brucella. Central Veterinary Laboratory, New Haw, Weybridge, Surrey, England. CUTLER, P., 1979. Problem solving in clinical medicine. From data to diagnosis. London: Williams and Wilkins Co. HERR, S., BISHOP, G., BOLTON, T. F. & VANDERMERWE,D., 1979. Onderstepoort brucellosis serology laboratory manual. Veterinary Research Institute, Onderstepoort, RSA. HERR, S. & Roux, D., 1981. The efficacy of bacteriological pro-cedures for the isolation of Brucella abortus from abattoir material. Onderstepoort Veterinary Journal, 48,7-12. HERR, S., WILLIAMSON, CATHERINE, C., PRIGGE, ROMY E. & VAN WYK, ANTONETIE, 1986. The relationship between the microtitra-tion serum agglutination and complement fixation tests in bovine brucellosis serology. Onderstepoort Journal of Veterinary Research, 53, 199-200. HERzBERG, M. & ELBERG, S. S., 1955. Immunization against Bru-cella infection. ill. Response of mice and guinea pigs to injection of viable and nonviable suspensions of a streptomycin-dependant mutant of Brucella melitensis. Journal of Bacteriology, 69, 432-435. MORGAN, W. J. B., MACKINNON, D. J., GD..L, K. P. W., GoWER, S. G. M. & NORRIS, P. I. W., 1978. Brucellosis diagnosis standard laboratory techniques. Ministry of Agriculture, Fisheries and Food, 2nd ed. Central Veterinary laboratory, New Haw, Weybridge, Sur-rey, England. PiETERSoN, P. M., GUMMOW, B., PEFANlS, S. , VENTER, CA'fHB. RINA, G. & HERR, S., 1988. The characteristics of a variant strain of Brucella melitensis Rev 1. Onderstepoort Journal of Veterinary Research, 55, 15--17. VAN DRIMMELEN, G. C. , 1960. Control of bruceUosis in sheep and goats by means of vaccination. Journal of the South African Vete-rinary Medical Association, 31, 129--138. 

The isolation and serology of the "FSA" Brucella melitensis Rev.1 mutant in a flock of sheep

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The isolation and serology of the "FSA" Brucella melitensis Rev. 1 mutant in a flock of sheep

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