Service-Profit Chain Analysis in Healthcare Services

Focus on service-profit chain by organizations in the service sector has been found to be of crucial importance. Companies in varied sectors like banking, airlines, restaurants and healthcare have become industry leaders by focusing on aspects of service-profit chain. This paper presents an analysis of service-profit chain in the healthcare sector. Taking two examples of hospitals from India and one from abroad this paper brings out the importance of focusing on the service-profit chain in this sector. An analysis of the practices in these hospitals, with a major focus on Aravind Eye Hospital, will give a perspective of how these hospitals have focused on the service-profit chain and made them efficient and effective and have enhanced their customers’ satisfaction. Service-profit chain analysis can help healthcare organizations to be customer focused. It can motivate organizations to develop attractive value propositions for customers. It can also provide a warning to organizations that are neglecting the interests of employees. Overall, the service-profit chain provides a useful framework for healthcare organizations in developing their strategy as well as implementing day-to-day operations

Evaluating Quality of Matrimonial Websites: Balancing Emotions with Economics

There are a plethora of studies evaluating the quality of websites on functional and design-related aspects such as usability and visual parameters. The majority of these studies are related to e-commerce websites where individuals make decision largely relying on economic parameters. However, matrimonial websites are unique, as the decisions involve both economic and non-economic parameters. Therefore, this study aims to propose a framework to evaluate quality of matrimonial websites by incorporating contextual factors and examine differences among different groups of users. This study proffers a website evaluating framework considering non-economic and emotion based factors from the information systems (IS) success model and the search match interaction (SMI) framework. The study proposes a hybrid model of multi-criteria decision-making techniques—namely Fuzzy-AHP and ranking models such as evaluation based on distance from average solution (EDAS), technique for order of preference by similarity to ideal solution (TOPSIS), and complex proportional assessment (COPRAS). The results indicate that the context-specific factors related to search and matchmaking options are the most preferred parameters for evaluation. Males and females have been found to differ in their preferences related to service quality and price. Next, the study compares the performance of three ranking models, namely EDAS, TOPSIS, and COPRAS. The first and second models provide similar results, while the rankings obtained through COPRAS differ slightly. The study contributes towards website evaluation literature by highlighting the importance of contextual factors while evaluating the matrimonial websites and the differences among preferences of the users

Cellular oxido-reductive proteins of Chlamydomonas reinhardtii control the biosynthesis of silver nanoparticles

<p>Abstract</p> <p>Background</p> <p>Elucidation of molecular mechanism of silver nanoparticles (SNPs) biosynthesis is important to control its size, shape and monodispersity. The evaluation of molecular mechanism of biosynthesis of SNPs is of prime importance for the commercialization and methodology development for controlling the shape and size (uniform distribution) of SNPs. The unicellular algae <it>Chlamydomonas reinhardtii </it>was exploited as a model system to elucidate the role of cellular proteins in SNPs biosynthesis.</p> <p>Results</p> <p>The <it>C. reinhardtii </it>cell free extract (<it>in vitro</it>) and <it>in vivo </it>cells mediated synthesis of silver nanoparticles reveals SNPs of size range 5 ± 1 to 15 ± 2 nm and 5 ± 1 to 35 ± 5 nm respectively. <it>In vivo </it>biosynthesized SNPs were localized in the peripheral cytoplasm and at one side of flagella root, the site of pathway of ATP transport and its synthesis related enzymes. This provides an evidence for the involvement of oxidoreductive proteins in biosynthesis and stabilization of SNPs. Alteration in size distribution and decrease of synthesis rate of SNPs in protein-depleted fractions confirmed the involvement of cellular proteins in SNPs biosynthesis. Spectroscopic and SDS-PAGE analysis indicate the association of various proteins on <it>C. reinhardtii </it>mediated <it>in vivo </it>and <it>in vitro </it>biosynthesized SNPs. We have identified various cellular proteins associated with biosynthesized (<it>in vivo </it>and <it>in vitro) </it>SNPs by using MALDI-MS-MS, like ATP synthase, superoxide dismutase, carbonic anhydrase, ferredoxin-NADP⁺reductase, histone etc. However, these proteins were not associated on the incubation of pre-synthesized silver nanoparticles <it>in vitro</it>.</p> <p>Conclusion</p> <p>Present study provides the indication of involvement of molecular machinery and various cellular proteins in the biosynthesis of silver nanoparticles. In this report, the study is mainly focused towards understanding the role of diverse cellular protein in the synthesis and capping of silver nanoparticles using <it>C. reinhardtii </it>as a model system.</p

Evaluating Quality of Matrimonial Websites: Balancing Emotions with Economics

There are a plethora of studies evaluating the quality of websites on functional and design-related aspects such as usability and visual parameters. The majority of these studies are related to e-commerce websites where individuals make decision largely relying on economic parameters. However, matrimonial websites are unique, as the decisions involve both economic and non-economic parameters. Therefore, this study aims to propose a framework to evaluate quality of matrimonial websites by incorporating contextual factors and examine differences among different groups of users. This study proffers a website evaluating framework considering non-economic and emotion based factors from the information systems (IS) success model and the search match interaction (SMI) framework. The study proposes a hybrid model of multi-criteria decision-making techniques—namely Fuzzy-AHP and ranking models such as evaluation based on distance from average solution (EDAS), technique for order of preference by similarity to ideal solution (TOPSIS), and complex proportional assessment (COPRAS). The results indicate that the context-specific factors related to search and matchmaking options are the most preferred parameters for evaluation. Males and females have been found to differ in their preferences related to service quality and price. Next, the study compares the performance of three ranking models, namely EDAS, TOPSIS, and COPRAS. The first and second models provide similar results, while the rankings obtained through COPRAS differ slightly. The study contributes towards website evaluation literature by highlighting the importance of contextual factors while evaluating the matrimonial websites and the differences among preferences of the users

Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae

Abstract Objective The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Results Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants

MOESM1 of Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae

Additional file 1. Recombinant CsR expressed in E. coli were probed with anti-Penta His Ab and anti-CsR Ab

STUDENTS’ FEEDBACK ON YOGA TRAINING AS PART OF THE FOUNDATION COURSE IN THE MBBS CURRICULUM.

Objective: The objective of this study is to take responses in the form of feedback by the students with regards yoga training during the foundation course. Methods: It was a cross-sectional study. After taking consent from the students, the data was collected using self-administered, semi-structured questionnaires. The feedback form with the foundation course was distributed to all the newly joined&nbsp; first&nbsp; year MBBS students&nbsp; of MGM Medical College, Jamshedpur, Jharkhand. India. Results: Majority (48.84%) of first year MBBS students liked Dhyana. All the participants agreed that yoga benefitted them. Conclusion: Yoga helped students reduce stress and anxiety improve overall physical health. It should be continued in MBBS foundation curriculum. &nbsp; &nbsp

Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems

Light-gated ion channel and ion pump rhodopsins are widely used as optogenetic tools and these can control the electrically excitable cells as (1) they are a single-component system i.e., their light sensing and ion-conducting functions are encoded by the 7-transmembrane domains and, (2) they show fast kinetics with small dark-thermal recovery time. In cellular signaling, a signal receptor, modulator, and the effector components are involved in attaining synchronous regulation of signaling. Optical modulation of the multicomponent network requires either receptor to effector encoded in a single ORF or direct modulation of the effector domain through bypassing all upstream players. Recently discovered modular rhodopsins like rhodopsin guanylate cyclase (RhoGC) and rhodopsin phosphodiesterase (RhoPDE) paves the way to establish a proof of concept for utilization of complex rhodopsin (modular rhodopsin) for optogenetic applications. Light sensor coupled modular system could be expressed in any cell type and hence holds great potential in the advancement of optogenetics 2.0 which would enable manipulating the entire relevant cell signaling system. Here, we had identified 50 novel modular rhodopsins with variant domains and their diverse cognate signaling cascades encoded in a single ORF, which are associated with specialized functions in the cells. These novel modular algal rhodopsins have been characterized based on their sequence and structural homology with previously reported rhodopsins. The presented novel modular rhodopsins with various effector domains leverage the potential to expand the optogenetic tool kit to regulate various cellular signaling pathways across the diverse biological model systems.https://doi.org/10.3390/life1011025

Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems

Light-gated ion channel and ion pump rhodopsins are widely used as optogenetic tools and these can control the electrically excitable cells as (1) they are a single-component system i.e., their light sensing and ion-conducting functions are encoded by the 7-transmembrane domains and, (2) they show fast kinetics with small dark-thermal recovery time. In cellular signaling, a signal receptor, modulator, and the effector components are involved in attaining synchronous regulation of signaling. Optical modulation of the multicomponent network requires either receptor to effector encoded in a single ORF or direct modulation of the effector domain through bypassing all upstream players. Recently discovered modular rhodopsins like rhodopsin guanylate cyclase (RhoGC) and rhodopsin phosphodiesterase (RhoPDE) paves the way to establish a proof of concept for utilization of complex rhodopsin (modular rhodopsin) for optogenetic applications. Light sensor coupled modular system could be expressed in any cell type and hence holds great potential in the advancement of optogenetics 2.0 which would enable manipulating the entire relevant cell signaling system. Here, we had identified 50 novel modular rhodopsins with variant domains and their diverse cognate signaling cascades encoded in a single ORF, which are associated with specialized functions in the cells. These novel modular algal rhodopsins have been characterized based on their sequence and structural homology with previously reported rhodopsins. The presented novel modular rhodopsins with various effector domains leverage the potential to expand the optogenetic tool kit to regulate various cellular signaling pathways across the diverse biological model systems

The Sialoside-Binding Pocket of SARS-CoV-2 Spike Glycoprotein Structurally Resembles MERS-CoV

COVID-19 novel coronavirus (CoV) disease caused by severe acquired respiratory syndrome (SARS)-CoV-2 manifests severe lethal respiratory illness in humans and has recently developed into a worldwide pandemic. The lack of effective treatment strategy and vaccines against the SARS-CoV-2 poses a threat to human health. An extremely high infection rate and multi-organ secondary infection within a short period of time makes this virus more deadly and challenging for therapeutic interventions. Despite high sequence similarity and utilization of common host-cell receptor, human angiotensin-converting enzyme-2 (ACE2) for virus entry, SARS-CoV-2 is much more infectious than SARS-CoV. Structure-based sequence comparison of the N-terminal domain (NTD) of the spike protein of Middle East respiratory syndrome (MERS)-CoV, SARS-CoV, and SARS-CoV-2 illustrate three divergent loop regions in SARS-CoV-2, which is reminiscent of MERS-CoV sialoside binding pockets. Comparative binding analysis with host sialosides revealed conformational flexibility of SARS-CoV-2 divergent loop regions to accommodate diverse glycan-rich sialosides. These key differences with SARS-CoV and similarity with MERS-CoV suggest an evolutionary adaptation of SARS-CoV-2 spike glycoprotein reciprocal interaction with host surface sialosides to infect host cells with wide tissue tropism.https://doi.org/10.3390/v1209090